LOCUS       V031979                10480 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031979
VERSION     V031979
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4506
                     /label="SLC6A15(NM_182767)"
                     /note="SLC6A15(NM_182767)"
                     /gene="SLC6A15"
     CDS             4513..4578
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4622..4833
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4846..5114
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5143..5152
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5173..5835
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5905..5958
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5959..6558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6559..7147
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7221..7454
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7526..7647
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7687..7822
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7855..7871)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7879..7895
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7903..7933)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7948..7969
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8257..8845)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9016..9876)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9877..9981)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10455..10471
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ccaaaaatag caaggtggta
     2341 aaaagagaat tagatgatga tgttactgag tctgtcaaag accttctttc caatgaagac
     2401 gcagctgatg atgcttttaa gacaagtgaa ctaattgttg atggccagga agagaaagat
     2461 acagatgttg aagaaggatc tgaagtcgaa gatgaaagac cagcttggaa cagtaaacta
     2521 caatacatcc tggcccaagt tggattttct gtaggtttag gaaatgtgtg gcgatttcca
     2581 tacctatgtc agaagaatgg gggcggtgca tatcttttac catatttaat actacttatg
     2641 gtaataggta ttcccctttt tttcttggaa ctctctgtgg gtcaaagaat tcggcgaggc
     2701 agcattggtg tatggaatta cataagccct aaactgggcg ggattggatt tgcaagttgt
     2761 gtagtgtgct attttgtagc tctctactac aacgtcatca ttggctggag tttgttttat
     2821 ttttctcagt cttttcagca acccctgcct tgggatcagt gtcctttggt gaaaaatgct
     2881 tcacacactt ttgtagaacc agaatgtgaa caaagttctg ccaccaccta ttactggtac
     2941 agggaagcac tgaatatttc aagttccatt tctgaaagtg ggggcttaaa ctggaagatg
     3001 accatctgct tgttggctgc ctgggtcatg gtttgcttgg ctatgatcaa aggcattcag
     3061 tcttctggaa aaatcatata ttttagttct ctgtttccat atgtggtact tatttgcttc
     3121 ctcatcagag cattcctttt aaatggttca attgatggca ttcgccacat gtttacccct
     3181 aagcttgaaa taatgctgga gcccaaggtc tggagagaag ctgctactca agtgttcttt
     3241 gccttaggtc tgggatttgg tggtgtcatt gccttttcaa gctacaacaa gagagacaac
     3301 aactgccact ttgatgctgt cctggtgtcc ttcatcaatt ttttcacttc tgtcctggca
     3361 acattggtgg tgtttgcagt tctgggcttc aaagcaaatg tcataaatga gaaatgcatt
     3421 acacaaaatt cagagacgat catgaaattt ttgaaaatgg ggaacattag tcaggatatt
     3481 attccccatc atatcaacct ttcaactgtt actgcagaag attatcattt agtttatgac
     3541 atcattcaaa aagtgaaaga agaagagttt cctgctcttc atctcaattc ctgtaaaatt
     3601 gaagaagagc taaataaagc tgttcagggg accggcttag cttttattgc ctttacagaa
     3661 gcgatgacac attttcctgc atctcccttc tggtcagtga tgtttttcct catgctggtc
     3721 aatctaggcc ttggcagtat gtttggaacc attgaaggga ttgtcacgcc tattgtggac
     3781 actttcaaag tgaggaaaga aattcttact gttatctgtt gtcttctggc attttgtatt
     3841 ggcctgatat ttgtgcaacg ctctggaaat tactttgtta caatgtttga tgattattct
     3901 gctacactgc ctctgctaat tgtagtcatt ttggagaata ttgctgtatg ctttgtttat
     3961 ggcatagata agtttatgga agacctaaaa gatatgctgg gctttgctcc cagcagatat
     4021 tactactata tgtggaaata tatttctcct ctaatgctat tatcattgct aatagctagt
     4081 gttgtgaata tgggattaag tcctcctggc tataacgcat ggattgaaga taaggcatct
     4141 gaagaatttc tgagctatcc aacatgggga ctggttgttt gtgtctctct ggttgtcttt
     4201 gcaatactcc cagtccctgt agttttcatt gttcgtcgct tcaaccttat agatgatagt
     4261 tctggtaatt tagcatctgt gacctataag agaggaaggg tcctgaaaga gcctgtgaac
     4321 ttagagggcg atgatacaag cctcattcac ggaaaaatac cgagcgagat gccatctcca
     4381 aattttggta aaaatattta tcgaaaacag agtggatccc caactctgga tactgctccc
     4441 aatggacggt atggaatagg gtacttgatg gcagatatta tgccagatat gccagaatct
     4501 gatttgggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4561 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     4621 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4681 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4741 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4801 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4861 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     4921 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     4981 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5041 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5101 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5161 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5221 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5281 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5341 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5401 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5461 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5521 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     5581 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     5641 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     5701 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     5761 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     5821 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     5881 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     5941 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6001 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6061 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6121 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6181 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6241 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6301 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6361 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6421 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6481 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6541 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6781 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6841 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6961 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7021 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7081 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7141 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7201 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     7261 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7321 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7381 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7441 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7501 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7621 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7681 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7741 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7801 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     7861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10021 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10081 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10141 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10201 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10261 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10321 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10381 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10441 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
