LOCUS       V045154                10120 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045154
VERSION     V045154
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4146
                     /label="SLC5A8(NM_145913)"
                     /note="SLC5A8(NM_145913)"
                     /gene="SLC5A8"
     CDS             4153..4218
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4262..4473
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4486..4754
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4783..4792
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4813..5475
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5545..5598
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5599..6198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6199..6787
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6861..7094
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7166..7287
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7327..7462
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7495..7511)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7519..7535
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7543..7573)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7588..7609
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7897..8485)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8656..9516)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9517..9621)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10095..10111
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg acacgccacg gggcatcggc
     2341 accttcgtgg tgtgggacta cgtggtgttc gcgggcatgc tggtcatctc ggccgccatc
     2401 ggcatctact acgccttcgc tgggggcggc cagcagacct ccaaggactt cctgatgggc
     2461 ggccgcagaa tgaccgcagt gcccgtggcg ctgtccctca ccgctagctt catgtcagcc
     2521 gtcactgtcc tgggcacccc ctccgaggtc taccgttttg gggccatttt tagcatcttt
     2581 gccttcacct acttctttgt ggtggtcatc agcgcggagg tcttcctccc ggtgttctac
     2641 aaactgggaa ttaccagcac ctacgagtat ttagaacttc gatttaacaa atgtgttcgt
     2701 ctctgtggaa cagtcctctt cattgttcaa acaattctgt atactggaat tgttatttat
     2761 gcccctgccc tggctttgaa tcaagtcaca ggatttgatc tgtggggcgc ggtagtggca
     2821 acgggggtgg tctgcacatt ctactgcaca ctgggtggtc ttaaagcagt tatctggaca
     2881 gatgtttttc aagttgggat catggtggct ggatttgcat ccgtgattat acaggctgtg
     2941 gtgatgcaag gtggaatcag cactatttta aatgatgcct atgatggtgg aagattaaat
     3001 ttctggaatt ttaatcctaa ccctttgcaa agacacacct tctggacaat tattatagga
     3061 gggaccttca catggaccag catctacggt gtcaaccaat cccaggtgca gagatatatt
     3121 tcttgtaaaa gcagattcca ggcaaaactg tctctctaca tcaatcttgt gggactctgg
     3181 gcaatcctca catgctcagt gttttgtggg ctcgccctat attccaggta ccatgactgt
     3241 gatccttgga cagccaagaa agtgtctgca ccagaccagc tcatgcctta tttggtactg
     3301 gacattctgc aagattatcc aggacttcct ggactttttg tggcctgtgc ttacagtggg
     3361 acattaagca cagtgtcctc cagtattaat gccttagcag cagtaactgt ggaagatcta
     3421 atcaaacctt acttcagatc gctctcagaa aggtctctgt cttggatttc ccaaggaatg
     3481 agtgtggtgt atggagccct gtgtattgga atggctgcgc tggcgtcact tatgggagct
     3541 ttgttgcagg cagcactcag cgtatttggt atggttggtg gaccacttat gggcctgttc
     3601 gctttgggca ttttggttcc ctttgccaac tcaattggag cacttgttgg tctgatggct
     3661 ggatttgcca tttctctatg ggttggaatt ggagctcaaa tatatcctcc acttcctgag
     3721 agaacattgc cattgcacct tgatatccaa ggctgtaaca gcacctacaa tgagacaaat
     3781 ttgatgacaa ccacagaaat gccatttact actagtgttt ttcaaatata caatgttcaa
     3841 aggactccac tgatggataa ctggtattct ttatcatatc tgtacttcag cactgttgga
     3901 actttggtaa cattattagt ggggatactt gtcagtttat caacaggagg aagaaaacag
     3961 aacttagacc ccagatacat actaaccaaa gaggactttt tatccaattt tgatattttt
     4021 aagaaaaaga agcatgtttt gagctataaa tcacatccag tggaagatgg tggaactgat
     4081 aatcctgctt tcaaccacat tgaattgaac tcagatcaga gtggcaagag caatgggact
     4141 cgtttgggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4201 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     4261 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4321 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4381 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4441 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4501 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     4561 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     4621 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     4681 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     4741 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     4801 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     4861 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     4921 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     4981 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5041 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5101 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5161 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     5221 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     5281 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     5341 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     5401 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     5461 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     5521 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     5581 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5641 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5701 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5761 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5821 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5881 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5941 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6001 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6061 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6121 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6181 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6601 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6781 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6841 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     6901 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6961 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7021 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7081 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7141 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7201 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7261 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7321 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7381 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7441 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     7501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7561 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7681 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7741 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7801 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7861 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7921 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7981 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8041 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8101 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8161 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8221 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8281 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8341 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8401 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8461 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8521 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8581 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9661 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     9721 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9781 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9841 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9901 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     9961 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10021 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10081 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
