LOCUS       V027824                 9694 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027824
VERSION     V027824
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4527
                     /label="SLC4A1AP(NM_018158)"
                     /note="SLC4A1AP(NM_018158)"
                     /gene="SLC4A1AP"
     CDS             4534..4560
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4564..4590
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4594..4620
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4664..4875
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4888..5156
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5183..5782
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5789..6377
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6451..6684
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6756..6877
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6896..7031
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7069..7085)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7093..7109
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7117..7147)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7162..7183
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7471..8059)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8230..9090)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9091..9195)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9669..9685
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggctgac attctctctc agtcagagac cctggcgtcg
     2341 caagacctca gtggggactt caagaagcca gctctgccgg tgtccccagc ggcgcggagt
     2401 aaggccccgg ccagcagttc ttcaaaccct gaggaggtac agaaggaagg gcccactgcg
     2461 ttgcaggact ccaattctgg ggagcccgac atccctcctc ctcagccgga ctgcggtgat
     2521 tttaggagtc tacaggagga gcagtcgcgc cccccgacag cggtttcttc ccctggcggt
     2581 ccagcccggg ctccccccta ccaagagcct ccatggggtg gccctgccac agccccctac
     2641 agcttagaga ccctgaaggg cggcactatc cttggcaccc gtagcttgaa agggacgagt
     2701 tactgccttt tcgggaggct gtctggctgc gacgtgtgcc tggagcaccc ttcggtgtct
     2761 cggtaccacg cagtgctgca gcacagggcg tccggccctg acggagaatg cgacagcaac
     2821 gggccgggct tctacctcta cgatctggga agcacccatg gcacttttct caacaaaact
     2881 cgcatcccac ctcgcaccta ctgtcgagtc cacgttgggc atgttgttcg ctttggaggc
     2941 agcacccggc tctttatcct gcagggacca gaggaagacc gagaggcaga atccgagtta
     3001 acagtaacac agttgaagga attgcgcaag cagcagcaaa tattgttgga gaagaagatg
     3061 ctaggagaag actcagatga agaagaggaa atggatacct ctgaaaggaa gataaatgct
     3121 ggtagccaag atgatgagat gggttgcacc tggggaatgg gagaagatgc agtagaggat
     3181 gatgctgaag agaaccctat tgtcttagag tttcagcagg aaagggaggc cttttatata
     3241 aaggatccca aaaaggctct ccaaggcttt tttgaccgag aaggagaaga attagaatat
     3301 gaatttgatg aacagggaca tagcacttgg ctctgcaggg tgagattacc tgtggacgat
     3361 tcaactggaa aacaactggt ggctgaggcc attcactcag gaaagaaaaa agaagcaatg
     3421 atccagtgct cattggaagc ttgtcggatt cttgacactt tgggattgct tcggcaggaa
     3481 gcagtatctc ggaaaaggaa agccaagaac tgggaagatg aagactttta tgatagtgat
     3541 gatgacacat ttcttgatag gactggcctg attgagaaga agcgtctgaa cagaatgaag
     3601 aaggctggca agattgatga gaagccagag acctttgaat cattggttgc aaaattaaat
     3661 gatgctgaaa gggaactttc tgaaatttct gagagattga aagcctcaag ccaagttcta
     3721 tcagagtctc catctcagga ttctttagat gcgttcatgt cagaaatgaa atcaggcagt
     3781 acattagatg gtgtgtcccg gaagaaactt cacctgagaa cttttgaact gaggaaagaa
     3841 caacagagac ttaaagggtt aataaaaatt gtaaagccag cagagattcc agaactaaaa
     3901 aagactgaaa ctcagactac aggtgcagaa aacaaagcta aaaagcttac attgcctcta
     3961 tttggtgcca tgaaaggagg aagcaaattc aaattaaaaa ctggaacagt agggaagtta
     4021 ccccccaagc gtccagaact ccctccaact ctaatgagaa tgaaagatga gcctgaagta
     4081 gaagaggagg aggaagagga agaggaagaa gagaaagaaa aggaggagca tgaaaagaaa
     4141 aaactggagg atggaagcct cagtaggcca cagccagaga tagagccaga agcagcagtg
     4201 caggaaatga ggcctcccac agatctcaca cattttaaag aaacccaaac ccatgaaaac
     4261 atgtctcaac ttagcgagga agaacagaat aaagattatc aagactgtag caaaaccact
     4321 tcattgtgcg caggaccctc agcatcaaag aatgaatatg agaaaagcag aggtgaattg
     4381 aagaaaaaga aaacacctgg tccaggcaaa cttccaccaa cactttcttc caaatatcct
     4441 gaagatgacc cagactactg tgtgtgggtc ccacctgaag gtcaaagtgg agatggcaga
     4501 acccatctta atgacaagta tggctatgga tcctacccat acgacgtacc tgattatgca
     4561 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     4621 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4681 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4741 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4801 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4861 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4921 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4981 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5041 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5101 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5161 ctgtgaccgg cgcctacgct agatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5221 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5281 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5341 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5401 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5461 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5521 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5581 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5641 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5701 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5761 gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5821 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5881 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5941 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6001 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6061 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6121 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6181 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6241 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6301 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6361 ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6421 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat
     6481 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6541 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6601 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6661 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6721 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6781 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6841 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6961 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7021 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt
     7081 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7141 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7201 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8221 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8281 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8341 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8401 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8461 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8521 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8581 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8641 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8701 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8761 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8821 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8881 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8941 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9001 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9061 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9241 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     9301 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     9361 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     9421 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9481 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     9541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     9601 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     9661 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
