LOCUS       V035602                11463 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035602
VERSION     V035602
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5650
                     /label="SLC4A10(NM_001178015)"
                     /note="SLC4A10(NM_001178015)"
                     /gene="SLC4A10"
     CDS             5672..6391
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        6433..6644
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6657..6925
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6952..7551
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7558..8146
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8220..8453
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8525..8646
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8665..8800
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8838..8854)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8862..8878
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8886..8916)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8931..8952
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9240..9828)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9999..10859)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10860..10964)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11438..11454
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg agattaaaga ccagggagcc caaatggagc cgctgctgcc
     2341 tacgagaaat gatgaagaag cagttgtgga tagaggtgga actcgttcta ttctcaaaac
     2401 acactttgag aaagaagatt tagaaggtca tcgaacacta tttattggag tacatgtgcc
     2461 cttgggagga agaaaaagcc atcgacgtca caggcatcgt ggtcataaac acagaaagag
     2521 agacagagaa agagattcag gattagagga tggaagggag tcaccttctt ttgacacccc
     2581 atcacagagg gtacagttta ttcttggaac cgaggatgat gacgaggaac acattcctca
     2641 tgaccttttc acagaactgg atgagatttg ttggcgtgaa ggtgaggacg ctgagtggcg
     2701 agaaacagcc aggtggttga agtttgaaga agatgtggaa gatggaggag aaaggtggag
     2761 caagccttat gtggctactc tttcattgca cagcttgttt gaattgagaa gttgtattct
     2821 gaatggaact gtgttgctgg acatgcatgc caacacttta gaagaaattg cagatatggt
     2881 tcttgaccaa caagtgagct caggtcagct gaatgaagat gtacgccata gggtccatga
     2941 ggcattgatg aaacagcatc atcatcagaa tcagaaaaaa ctcaccaaca ggattcccat
     3001 tgttcgttcc tttgctgata ttggcaagaa acagtcagaa ccaaattcca tggacaaaaa
     3061 tgcaggtcag gttgtttctc ctcagtctgc tccagcctgt gttgaaaata aaaatgatgt
     3121 tagcagagaa aacagcactg ttgactttag caagggactg ggaggccaac aaaaggggca
     3181 tactagtcca tgtgggatga aacaaaggca tgaaaaagga cctccacacc agcaagagag
     3241 agaggttgat ctgcatttta tgaaaaagat tcctccaggt gctgaagcat cgaacatctt
     3301 agtgggagaa ctggagttct tggatcgaac agtagttgcg tttgtcaggt tgtctccagc
     3361 tgtattgctt caaggactgg ctgaagtccc aatcccaacc agatttttgt tcattcttct
     3421 gggacccctg ggaaagggtc aacagtacca tgagattggc agatcaattg caaccctaat
     3481 gacagatgag gtatttcatg atgttgccta taaagctaaa gatcgtaatg acttggtatc
     3541 aggaattgat gagtttctgg atcaggttac tgttctccct cctggagaat gggatccaag
     3601 cattcgaata gagcctccca aaaatgttcc ttcccaggag aagaggaaga ttcctgctgt
     3661 accaaatgga acagcagctc atggggaagc agagccccac ggaggacata gtggacctga
     3721 actccagcga actggaagga tttttggggg acttatttta gatatcaaaa gaaaagctcc
     3781 atacttctgg agtgacttca gagatgcttt cagcctgcag tgcttagcat cttttctatt
     3841 tctctactgc gcgtgtatgt ctcctgtcat cacgtttgga ggactgctgg gagaagcaac
     3901 tgaagggcgt ataagtgcaa ttgaatctct ctttggagca tccatgaccg ggatagccta
     3961 ttctctcttt ggtggacagc ctcttaccat attaggcagt acaggaccag ttttggtgtt
     4021 tgaaaagatt ttgtttaaat tttgcaaaga atatgggctg tcatacctat ctttaagagc
     4081 tagcattgga ctttggactg caactctatg tatcatactt gtggccacag atgctagttc
     4141 ccttgtctgc tacatcactc ggtttactga agaagctttt gcttccctga tttgcatcat
     4201 tttcatttat gaggccctgg agaagttgtt tgaactcagt gaagcatatc caatcaacat
     4261 gcataatgat ctggaactgc tgacacaata ctcgtgtaac tgtgtggaac cgcataatcc
     4321 cagcaatggc acattgaagg aatggaggga atccaatatt tctgcctctg acataatttg
     4381 ggagaaccta actgtgtcag aatgcaaatc attgcatgga gagtatgttg gacgggcctg
     4441 tggccatgat cacccatatg ttccagatgt tctattttgg tctgtgatcc tgttcttttc
     4501 cacagttact ctgtcagcca ccctgaagca gttcaagact agcagatatt ttccaaccaa
     4561 ggttcgatcc atagtgagtg actttgctgt ctttcttaca attctgtgta tggttttaat
     4621 tgactatgcc attgggatcc catctccaaa actacaagta ccaagtgttt tcaagcccac
     4681 tagagatgat cgtggctggt ttgttacgcc tttaggtcca aacccatggt ggacagtaat
     4741 agctgctata attccagctc tgctttgtac tattctaatt tttatggacc aacagattac
     4801 agctgtcatc atcaacagga aagagcataa gctaaagaaa ggttgtgggt accatctgga
     4861 cctattaatg gtggctgtca tgctcggtgt atgctccatc atgggcctgc catggtttgt
     4921 ggctgccaca gtcctctcca tcactcatgt caatagccta aaactggaat cagaatgctc
     4981 agctccagga gaacaaccca aatttctcgg cattcgggag caaagggtta ctgggcttat
     5041 gatttttatt cttatgggtt catcagtctt tatgaccagt attctgaagt ttattcccat
     5101 gccagtgcta tatggagtgt ttctttatat gggtgcttca tctctaaagg gaattcagtt
     5161 ctttgatagg ataaagctct tctggatgcc ggcaaaacat caaccagatt ttatatacct
     5221 aaggcacgta ccgcttcgaa aagtgcatct cttcacaatt attcagatga gttgccttgg
     5281 ccttttgtgg ataataaaag tttcaagagc tgctattgtc tttcccatga tggtgttagc
     5341 cctggtattt gtaagaaagt tgatggactt gttgttcacg aagcgggaac tcagctggtt
     5401 ggatgatttg atgcccgaga gtaagaaaaa gaaactggaa gatgctgaaa aagaagaaga
     5461 acaaagtatg ctagctatgg aagatgaggg cacagtacaa ctcccattgg aagggcacta
     5521 tagagatgat ccatctgtga tcaatatatc tgatgaaatg tcaaagactg ccttgtggag
     5581 gaaccttctg attactgccg ataactcaaa agataaggag tcaagctttc cttccaaaag
     5641 ctccccttcc ggatccggag gagggggaag catggtgagc aagggcgagg agctgttcac
     5701 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5761 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5821 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     5881 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5941 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     6001 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     6061 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6121 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     6181 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6241 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6301 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6361 cactctcggc atggacgagc tgtacaagta agcggccgcg aaggatctgc gatcgctccg
     6421 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     6481 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     6541 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     6601 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     6661 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     6721 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     6781 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     6841 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     6901 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag
     6961 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
     7021 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
     7081 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7141 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7201 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7261 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7321 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7381 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7441 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7501 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat
     7561 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7621 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7681 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7741 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7801 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7861 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7921 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7981 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8041 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8101 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8221 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct
     8281 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8341 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8401 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8461 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8521 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8581 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8641 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
     8701 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8761 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc
     8821 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8881 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8941 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9001 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9661 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9901 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9961 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10021 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10081 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10141 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10201 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10261 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10321 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10381 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10441 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10501 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10561 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10621 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10681 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10741 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10801 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10861 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10921 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10981 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    11041 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    11101 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    11161 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    11221 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    11281 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    11341 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    11401 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    11461 ctg
//
