LOCUS       V031786                 9626 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031786
VERSION     V031786
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4458
                     /label="SLC44A5(NM_152697)"
                     /note="SLC44A5(NM_152697)"
                     /gene="SLC44A5"
     CDS             4465..4491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4495..4521
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4525..4551
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4596..4807
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4820..5088
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5115..5714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5721..6309
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6383..6616
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6688..6809
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6828..6963
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7001..7017)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7025..7041
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7049..7079)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7094..7115
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7403..7991)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8162..9022)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9023..9127)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9601..9617
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaatgac acagaaaaac cagcagatac tccctctgag
     2341 gaagaggact ttggtgatcc aaggacatat gacccagatt tcaaggggcc tgttgccaac
     2401 aggagttgta cagatgttct gtgctgtatg atcttcctac tgtgtattat tggctacatt
     2461 gttttaggac ttgtggcctg ggtacatggg gaccccagaa gagcagccta tcctacagac
     2521 agccagggcc acttttgtgg ccagaagggc actcccaatg agaacaagac cattttgttt
     2581 tactttaacc tgttacgctg taccagtccc tccgtgttgc taaacctaca gtgccctacc
     2641 acacagatct gtgtctccaa gtgcccagaa aaatttttaa cctatgtgga aatgcaactt
     2701 ttgtacacaa aagacaaaag ctactgggaa gactaccgtc agttctgtaa gaccactgct
     2761 aagcctgtga agtctctcac acagctttta ctggatgatg attgtccaac agcgattttt
     2821 cccagcaaac cttttctcca gagatgtttc cctgacttct ctaccaaaaa tggcacttta
     2881 acaataggaa gtaagatgat gtttcaagat ggaaatggag ggacaagaag tgttgtagaa
     2941 ctcgggattg ctgcaaatgg tatcaataaa cttcttgatg caaagtcact tggattgaaa
     3001 gtgtttgaag actatgcaag aacttggtat tggattctca ttggcctgac gattgccatg
     3061 gtccttagtt ggatattttt gatacttctg aggttcatag ctggatgcct cttctgggtc
     3121 ttcatgattg gtgtgattgg aattataggt tatggaatat ggcactgtta ccagcagtac
     3181 accaatcttc aggaacgccc aagttctgta ttaactatct atgacatcgg gattcagact
     3241 aacataagca tgtactttga actgcaacaa acatggttca catttatgat aatactctgc
     3301 atcattgaag tgattgtcat cctcatgctg atcttcctca ggaatcgaat ccgagtcgcc
     3361 attatcctgc tgaaggaagg aagcaaagcc attggatatg ttcctagtac attagtctat
     3421 ccagctttaa ctttcatttt gctctcaatc tgcatttgct actgggtcgt gacagcagtt
     3481 ttcttggcga catcgggggt acctgtatac aaagtcatag ctccaggggg gcattgtata
     3541 catgaaaatc aaacctgtga cccagagatt tttaatacaa ctgaaattgc caaagcttgc
     3601 cctggggctc tgtgtaactt tgctttctat ggtggaaaga gcttgtacca tcagtacatc
     3661 cctaccttcc atgtatacaa cttatttgtc tttctctggc ttataaactt cgtcattgca
     3721 ttaggtcagt gcgcccttgc tggtgcattc gctacttatt actgggccat gaaaaaacct
     3781 gatgacatcc cacgatatcc actttttact gcatttggac gagccatacg atatcacaca
     3841 ggatccctag catttggatc tttaattatt gcattaattc aaatgtttaa aattgtacta
     3901 gaatacttgg accaccgtct taaacgtacc cagaacacat tgtctaaatt cctacaatgc
     3961 tgcctgagat gctgcttctg gtgtttggaa aatgcaataa agtttttaaa cagaaatgcc
     4021 tatattatga ttgcaatata tggcagaaac ttctgcaggt cagcaaaaga tgctttcaat
     4081 ctgctgatga gaaatgtttt gaaagttgca gttacagatg aagttacata ctttgtatta
     4141 ttcctgggga aacttctagt tgctggaagt ataggtgttc tggccttcct attcttcaca
     4201 caaagactgc cagtgattgc acaaggacca gcatctttaa attactactg ggtacctttg
     4261 ctgacagtca tttttgggtc ttacctgatt gcacatgggt tcttcagcgt ctatgcaatg
     4321 tgtgttgaaa caattttcat ctgcttctgt gaagatctgg aaagaaatga tggatctaca
     4381 gaaaaaccct acttcgtaac ccctaacctg catggaattc tgatcaagaa gcaactagtt
     4441 ccccagaagc agaaagaggg atcctaccca tacgacgtac ctgattatgc aacttatcct
     4501 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc ataggcggcc
     4561 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4621 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     4681 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4741 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4801 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     4861 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     4921 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     4981 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     5041 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     5101 ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     5161 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     5221 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5281 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5341 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5401 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5461 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5521 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5581 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5641 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5701 agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5761 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5821 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5881 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5941 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6001 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6061 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6121 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6181 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6241 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6301 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6361 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     6421 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6481 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6541 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6601 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6661 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6781 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6841 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6901 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6961 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     7021 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7081 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7141 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7201 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7801 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9121 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9181 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9241 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9301 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9361 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9421 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     9481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     9541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     9601 gtaaaacgac ggccagtgcc aagctg
//
