LOCUS       V046816                 9940 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046816
VERSION     V046816
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4035
                     /label="SLC43A2(NM_001284498)"
                     /note="SLC43A2(NM_001284498)"
                     /gene="SLC43A2"
     promoter        4099..4310
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4323..4591
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4620..4629
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4650..5312
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5382..5435
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5436..6035
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6036..6623
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6697..6930
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7002..7123
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7142..7277
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7315..7331)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7339..7355
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7363..7393)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7408..7429
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7717..8305)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8476..9336)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9337..9441)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9915..9931
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgcccaccct ggccactgcc
     2341 catcggcgcc gctggtggat ggcctgcacg gccgtgctgg agaacctcct cttctcggca
     2401 gtcctcctgg gctggggctc gctgctcatc atgctcaagt cagagggctt ttactcctac
     2461 ctgtgtaccg agccagagaa tgtcaccaat ggcacagtgg gcggcacagc agagccgggg
     2521 cacgaggagg tgagctggat gaacggctgg ctcagctgcc aggcccagga cgagatgcta
     2581 aatttggcct tcactgtggg ctcctttctg ctcagtgcca tcaccctgcc cctgggtatc
     2641 gtcatggaca agtatggccc gaggaagctc aggctgctgg gcagcgcctg cttcgcggtt
     2701 tcctgcttgc tgattgcgta cggagcaagt aaaccaaacg ctctctccgt gctcatcttc
     2761 atcgccctgg ctctgaatgg ctttggtggg atgtgtatga ccttcacctc attaacactg
     2821 cccaacatgt tcggcgacct tcggtccacg tttattgcct tgatgattgg gtcctacgcc
     2881 tcctcggcag tcacctttcc aggaatcaag ctcatctatg atgctggtgt ctccttcatc
     2941 gtcgtcctcg tggtctgggc cggctgctcc gggctggttt tcctcaactg cttctttaac
     3001 tggccccttg agcccttccc ggggccggag gacatggact actcggtgaa gatcaagttc
     3061 agctggctgg gctttgacca caagatcaca gggaagcagt tctacaagca ggtgaccacg
     3121 gtgggccggc gcctgagtgt gggcagctcc atgaggagtg ccaaggagca ggtggcgctg
     3181 caggagggcc acaagctgtg cctgtccacc gtcgacctgg aggtgaagtg ccagccggat
     3241 gccgcagtgg ccccctcctt catgcacagc gtgttcagcc ccatcctgct gctcagcctg
     3301 gtcaccatgt gcgtcacgca gctgcggctc atcttctaca tgggggctat gaacaacatc
     3361 ctcaagttcc tggtcagcgg cgaccagaag acagtgatgg ccacggttgg cctctacacc
     3421 tccatcttcg gcgtgctcca gctgctgtgc ctgctgacgg cccccgtcat tggctacatc
     3481 atggactgga ggctgaagga gtgtgaagac gcctccgagg agcccgagga gaaagacgcc
     3541 aaccaaggcg agaagaaaaa gaagaagcgg gaccggcaga tccagaagat cactaatgcc
     3601 atgcgggcct tcgccttcac caacctgctg ctcgtgggct ttggggtgac ctgcctcatt
     3661 cccaacctgc ctctccagat cctctccttc atcctgcaca caatcgtgcg aggattcatc
     3721 cactccgctg tcgggggcct gtacgctgcc gtgtacccct ccacccagtt cggcagcctc
     3781 acgggactgc agtctctgat cagcgcgctc ttcgcccttc tgcagcagcc gctgtttctg
     3841 gccatgatgg gtcctctcca gggagaccct ctgtgggtga acgtggggct gctccttctc
     3901 agcctgctgg gcttctgcct cccgctctac ctgatctgct accggcgcca gctggagcgg
     3961 cagctgcagc agaggcagga ggatgacaaa ctcttcctca aaatcaacgg ctcgtccaac
     4021 caggaggcct tcgtgtagga tccctgcgat atccagcgcg gccgcgaagg atctgcgatc
     4081 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4141 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4201 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4261 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     4321 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     4381 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     4441 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     4501 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     4561 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg
     4621 ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg
     4681 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg
     4741 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc
     4801 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga
     4861 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg
     4921 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg
     4981 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga
     5041 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg
     5101 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca
     5161 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt
     5221 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc
     5281 agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg
     5341 ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc
     5401 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc
     5461 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     5521 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     5581 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5641 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5701 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5761 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5821 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     5881 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5941 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6001 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt
     6061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6421 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6541 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga
     6601 tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     6661 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6721 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6901 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6961 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7021 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7081 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7141 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7201 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7261 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     7321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9481 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     9541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9661 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9721 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     9781 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9841 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9901 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
