LOCUS       V026146                10096 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026146
VERSION     V026146
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4122
                     /label="SIGLEC11(NM_001135163)"
                     /note="SIGLEC11(NM_001135163)"
                     /gene="SIGLEC11"
     CDS             4129..4194
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4238..4449
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4462..4730
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4759..4768
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4789..5451
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5521..5574
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5575..6174
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6175..6763
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6837..7070
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7142..7263
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7303..7438
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7471..7487)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7495..7511
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7519..7549)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7564..7585
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7873..8461)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8632..9492)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9493..9597)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10071..10087
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tcccgggaca ggcccagccc
     2341 cagagcccag agatgctgct gctgcccctg ctgctgcccg tgctgggggc ggggtccctg
     2401 aacaaggatc ccagttacag tcttcaagtg cagaggcagg tgccggtgcc ggagggcctg
     2461 tgtgtcatcg tgtcttgcaa cctctcctac ccccgggatg gctgggacga gtctactgct
     2521 gcttatggct actggttcaa aggacggacc agcccaaaga cgggtgctcc tgtggccact
     2581 aacaaccaga gtcgagaggt ggaaatgagc acccgggacc gattccagct cactggggat
     2641 cccggcaaag ggagctgctc cttggtgatc agagacgcgc agagggagga tgaggcatgg
     2701 tacttctttc gggtggagag aggaagccgt gtgagacata gtttcctgag caatgcgttc
     2761 tttctaaaag taacagccct gactaagaag cctgatgtct acatccccga gaccctggag
     2821 cccgggcagc cggtgacggt catctgtgtg tttaactggg ctttcaagaa atgtccagcc
     2881 ccttctttct cctggacggg ggctgccctc tcccctagaa gaaccagacc aagcacctcc
     2941 cacttctcag tgctcagctt cacgcccagc ccccaggacc acgacaccga cctcacctgc
     3001 catgtggact tctccagaaa gggtgtgagc gcacagagga ccgtccgact ccgtgtggcc
     3061 tatgccccca aagaccttat tatcagcatt tcacatgaca acacgtcagc cctggaactc
     3121 cagggaaacg tcatatatct ggaagttcag aaaggccagt tcctgcggct cctctgtgct
     3181 gctgacagcc agccccctgc cacgctgagc tgggtcctgc aggacagagt cctctcctcg
     3241 tcccacccct ggggccccag aaccctgggg ctggagctgc gtggggtaag ggccggggat
     3301 tcagggcgct acacctgccg agcggagaac aggcttggct cccagcagca agccctggac
     3361 ctctctgtgc agtatcctcc agagaacctg agagtgatgg tttcccaagc aaacaggaca
     3421 gtcctggaaa acctcgggaa cggcacatcc ctcccggtcc tggagggcca aagcctgcgc
     3481 ctggtctgtg tcacccacag cagcccccca gccaggctga gctggacccg gtggggacag
     3541 accgtgggcc cctcccagcc ctcagacccc ggggtcctgg agctgccacc cattcaaatg
     3601 gagcacgaag gagagttcac ctgccacgct cagcaccctc tgggctccca gcacgtctct
     3661 ctcagcctct ccgtgcactg gaagctggag catgggggag gacttggcct gggggctgcc
     3721 ctgggagctg gcgtcgctgc cctgctcgct ttctgttcct gccttgtcgt cttcagggtg
     3781 aagatctgca ggaaggaagc tcgcaagagg gcagcagctg agcaggacgt gccctccacc
     3841 ctgggaccca tctcccaggg tcaccagcat gaatgctcgg caggcagctc ccaagaccac
     3901 ccgcccccag gtgcagccac ctacaccccg gggaaggggg aagagcagga gctccactat
     3961 gcctccctca gcttccaggg cctgaggctc tgggagcctg cggaccagga ggcccccagc
     4021 accaccgagt actcggagat caagatccac acaggacagc ccctgagggg cccaggcttt
     4081 gggcttcaat tggagaggga gatgtcaggg atggttccaa agggatccga ctacaaagac
     4141 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4201 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4261 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4321 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4381 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4441 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4501 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4561 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4621 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     4681 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     4741 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     4801 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     4861 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     4921 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     4981 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5041 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5101 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5161 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5221 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5281 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5341 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5401 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5461 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5521 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5581 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5641 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5701 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5761 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5821 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5881 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5941 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6001 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6061 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6121 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6181 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6241 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6301 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6361 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6421 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6481 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6541 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6601 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6661 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     6721 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     6781 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6841 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     6901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7081 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7141 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7261 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7321 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7381 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7441 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     7501 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7561 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7621 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7681 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8281 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8821 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8881 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8941 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9001 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9061 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9121 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9181 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9241 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9301 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9361 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9421 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9481 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9541 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9601 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9661 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9721 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9781 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     9841 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     9901 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     9961 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10021 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10081 ggccagtgcc aagctg
//
