LOCUS       V022870                10093 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022870
VERSION     V022870
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4986
                     /label="SF3B2(NM_006842)"
                     /note="SF3B2(NM_006842)"
                     /gene="SF3B2"
     CDS             4993..5019
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5063..5274
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5287..5555
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5582..6181
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6188..6776
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6850..7083
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7155..7276
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7295..7430
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7468..7484)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7492..7508
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7516..7546)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7561..7582
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7870..8458)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8629..9489)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9490..9594)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10068..10084
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcgacg gagcatcccg agcctcccaa agcagaattg
     2341 cagctgccgc cgccgccacc tccaggccac tatggcgcct gggctgccca ggagcttcag
     2401 gccaagttgg cagagatcgg agctccgatc cagggtaatc gcgaggagct ggtggagcgg
     2461 ctgcagagct acacccgcca gactggcatc gtgctgaatc ggccggtttt gagaggggaa
     2521 gatggggaca aagccgctcc acctcccatg tcggcacagc tccctggaat tcccatgcca
     2581 ccaccacctt tgggactccc ccctctgcag cctcctccgc cacccccacc acctccacca
     2641 ggccttggcc ttggctttcc tatggcccac ccaccaaatt tggggccccc gcctcctctc
     2701 cgtgtgggtg agccagtggc actgtcagag gaggagcggc tgaagttggc tcagcagcag
     2761 gcggcattgc tgatgcagca ggaggagcgt gccaagcagc agggagatca ttcgctgaag
     2821 gaacatgagc tcttggagca gcagaagcgg gcagctgtgt tactggagca ggaacgacag
     2881 caggagattg ccaagatggg caccccagtc cctcggcccc cacaagacat gggccagatt
     2941 ggtgtgcgca ctcctctggg tcctcgagta gctgctccag tgggcccagt gggccccact
     3001 cctacagttt tgcccatggg agcccctgtt ccccggcctc gtggtccccc accgccccct
     3061 ggagatgaga acagagagat ggatgacccc tctgtgggcc ccaagatccc ccaggctttg
     3121 gagaagatcc tgcagctgaa ggagagccgc caggaagaga tgaattctca gcaggaggaa
     3181 gaggaaatgg aaacagatgc tcgctcgtcc ctgggccagt cagcgtcaga gactgaggag
     3241 gacacagtgt ccgtatctaa aaaggagaaa aaccggaagc gtaggaaccg aaagaagaag
     3301 aaaaagcccc agcgggtgcg aggggtgtcc tctgagagct ctggggaccg ggagaaagac
     3361 tcaacccggt cccgtggctc tgattcccca gcagctgatg ttgagattga gtatgtgact
     3421 gaagaacctg aaatttacga gcccaacttt atcttcttta agaggatctt tgaggctttt
     3481 aagctcactg atgatgtgaa gaaggagaaa gagaaggagc cagagaaact tgacaaactg
     3541 gagaactctg cagcccccaa gaagaaggga tttgaagagg agcacaagga cagtgatgat
     3601 gacagcagtg atgacgagca ggaaaagaag ccagaagccc ccaagctgtc caagaagaag
     3661 ttgcgccgaa tgaaccgctt cactgtggct gaactcaagc agctggtggc tcggcccgat
     3721 gtcgtggaga tgcacgatgt gacagcgcag gaccctaagc tcttggttca cctcaaggcc
     3781 actcggaact ctgtgcctgt gccacgccac tggtgtttta agcgcaaata cctgcagggc
     3841 aaacggggca ttgagaagcc ccccttcgag ctgccagact tcatcaaacg cacaggcatc
     3901 caggagatgc gagaggccct gcaggagaag gaagaacaga agaccatgaa gtcaaaaatg
     3961 cgagagaaag ttcggcctaa gatgggcaaa attgacatcg actaccagaa actgcatgat
     4021 gccttcttca agtggcagac caagccaaag ctgaccatcc atggggacct gtactatgag
     4081 gggaaggagt tcgagacacg actgaaggag aagaagccag gagatctgtc tgatgagcta
     4141 aggatttcct tggggatgcc agtaggacca aatgcccaca aggtccctcc cccatggctg
     4201 attgccatgc agcgatatgg accaccccca tcgtatccca acctgaaaat ccctgggctg
     4261 aactcgccca tccctgagag ctgttccttt gggtaccatg ctggtggctg gggcaaacct
     4321 ccagtggatg agactgggaa accgctctat ggggacgtgt ttggaaccaa tgctgctgaa
     4381 tttcagacca agactgagga agaagagatt gatcggaccc cttgggggga actggaacca
     4441 tctgatgaag aatcctcaga agaagaggaa gaggaagaaa gtgatgaaga caaaccagat
     4501 gagacaggct ttattacccc tgcagacagt ggccttatca ctcctggagg cttttcatca
     4561 gtgcctgctg gaatggagac ccctgaactc attgagctga ggaagaagaa gattgaggag
     4621 gcgatggacg gaagtgagac acctcagctc ttcactgtgt tgccagagaa gagaacagcc
     4681 actgttggag gggccatgat gggatcaacc cacatttatg acatgtccac ggttatgagc
     4741 cggaagggcc cggctcctga gctgcaaggt gtggaagtgg cgctggcgcc tgaagagttg
     4801 gagctggatc ctatggccat gacccagaag tatgaggagc atgtgcggga gcagcaggct
     4861 caagtagaga aggaggactt cagtgacatg gtggctgagc acgctgccaa acagaagcaa
     4921 aaaaaacgga aagctcagcc ccaggacagc cgtgggggca gcaagaaata taaggagttc
     4981 aagtttggat cctacccata cgacgtacca gattacgctt agcggccgcg aaggatctgc
     5041 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5101 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5161 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5221 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5281 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5341 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5401 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5461 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5521 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5581 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     5641 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     5701 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5761 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5821 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5881 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5941 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6001 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6061 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6121 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6181 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6241 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6301 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6361 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6421 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6481 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6541 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6601 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6661 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6721 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6781 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6841 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     6901 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6961 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7021 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7081 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7141 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7201 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7261 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     7321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7381 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     7441 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7501 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7561 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7621 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7681 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7741 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7801 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7861 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7921 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7981 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8041 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8101 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8161 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8221 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8281 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8341 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8401 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8461 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8521 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8581 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8641 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8701 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8761 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8821 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8881 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8941 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9001 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9061 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9121 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9181 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9241 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9301 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9361 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9421 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9481 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9541 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9601 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9661 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9721 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9781 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9841 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9901 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9961 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10021 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10081 cagtgccaag ctg
//
