LOCUS       V025262                10151 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025262
VERSION     V025262
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4194
                     /label="SAMHD1(NM_015474)"
                     /note="SAMHD1(NM_015474)"
                     /gene="SAMHD1"
     CDS             4201..4266
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4310..4521
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4534..4802
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4831..4840
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4861..5523
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5593..5646
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5647..6246
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6247..6834
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6908..7141
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7213..7334
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7353..7488
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7526..7542)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7550..7566
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7574..7604)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7619..7640
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7928..8516)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8687..9547)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9548..9652)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10126..10142
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agcgagccga ttccgagcag
     2341 ccctccaagc gtccccgttg cgatgacagc ccgagaaccc cctcaaacac cccttccgca
     2401 gaggcagact ggtccccggg cctggaactc catcccgact acaagacatg gggtccggag
     2461 caggtgtgct ccttcctcag gcgcggtggc tttgaagagc cggtgctgct gaagaacatc
     2521 cgagaaaatg aaatcacagg cgcattactg ccttgtcttg atgagtctcg ttttgaaaat
     2581 cttggagtaa gttccttggg ggagaggaag aagctgctta gttatatcca gcgattggtt
     2641 caaatccacg ttgatacaat gaaggtaatt aatgatccta tccatggcca cattgagctc
     2701 caccctctcc tcgtccgaat cattgataca cctcaatttc aacgtcttcg atacatcaaa
     2761 cagctgggag gtggttacta tgtttttcca ggagcttcac acaatcgatt tgagcatagt
     2821 ctaggggtgg ggtatctagc aggatgtcta gttcacgcac tgggtgaaaa acaaccagag
     2881 ctgcagataa gtgaacgaga tgttctctgt gttcagattg ctggactttg tcatgatctc
     2941 ggtcatgggc cattttctca catgtttgat ggacgattta ttccacttgc tcgcccggag
     3001 gtgaaatgga cgcatgaaca aggctcagtt atgatgtttg agcaccttat taattctaat
     3061 ggaattaagc ctgtcatgga acaatatggt ctcatccctg aagaagatat ttgctttata
     3121 aaggaacaaa ttgtaggacc acttgaatca cctgtcgaag attcattgtg gccatataaa
     3181 gggcgtcctg aaaacaaaag cttcctttat gagatagtat ctaataaaag aaatggcatt
     3241 gatgtggaca aatgggatta ttttgccagg gactgccatc atcttggaat ccaaaataat
     3301 tttgattaca agcgctttat taagtttgcc cgtgtctgtg aagtagacaa tgagttgcgt
     3361 atttgtgcta gagataagga agttggaaat ctgtatgaca tgttccacac tcgcaactct
     3421 ttacaccgta gagcttatca acacaaagtt ggcaacatta ttgatacaat gattacagat
     3481 gctttcctca aagcagatga ctacatagag attacaggtg ctggaggaaa aaagtatcgc
     3541 atttctacag caattgacga catggaagcc tatactaagc tgacagataa catttttctg
     3601 gagattttat actctactga tcccaaattg aaagacgcac gagagatttt aaaacaaatt
     3661 gaataccgta atctattcaa gtatgtgggt gagacgcagc caacaggaca aataaagatt
     3721 aaaagggagg actatgaatc tcttccaaaa gaggttgcca gtgctaaacc caaagtattg
     3781 ctagacgtga aactgaaggc tgaagatttt atagtggatg ttatcaacat ggattatgga
     3841 atgcaagaaa agaatccaat tgatcatgtt agcttctatt gtaagactgc ccccaacaga
     3901 gcaatcagga ttactaaaaa ccaggtttca caacttctgc cagagaaatt tgcagagcag
     3961 ctgattcgag tatattgtaa gaaggtggac agaaagagtt tgtatgccgc aagacaatat
     4021 tttgttcagt ggtgtgcaga cagaaatttc accaagccgc aggatggcga tgttatagcc
     4081 ccactcataa cacctcaaaa aaaggaatgg aacgacagta cttcagtcca aaatccaact
     4141 cgcctccgag aagcatccaa aagcagagtc cagcttttta aagatgaccc aatgggatcc
     4201 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     4261 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4321 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4381 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4441 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4501 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4561 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4621 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4681 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4741 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     4801 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     4861 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     4921 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     4981 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5041 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5101 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5161 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5221 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5281 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5341 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5401 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5461 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5521 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5581 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     5641 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5701 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5761 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5821 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5881 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5941 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6001 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6061 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6121 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6181 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6241 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6781 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta
     6841 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6901 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     6961 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7021 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7081 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7141 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7201 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7261 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7321 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7381 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7441 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     7501 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7561 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7621 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7681 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7741 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7801 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7861 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7921 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7981 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8041 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8101 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8161 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8221 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8281 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8341 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8401 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8461 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8521 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8581 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8641 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8701 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8761 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8821 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8881 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8941 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9001 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9061 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9121 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9181 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9241 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9301 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9361 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9421 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9481 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9541 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9601 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9661 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9721 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9781 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9841 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9901 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9961 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10021 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10081 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10141 gtgccaagct g
//
