LOCUS V038986 7685 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038986 VERSION V038986 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..2604 /label="S100a4(NM_011311)" /note="S100a4(NM_011311)" /gene="S100a4" promoter 2655..2866 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 2879..3147 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3174..3773 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 3780..4368 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4442..4675 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 4747..4868 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 4887..5022 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5060..5076) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5084..5100 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5108..5138) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5153..5174 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5462..6050) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6221..7081) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7082..7186) /gene="bla" /label="AmpR promoter" primer_bind 7660..7676 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcaaga cccttggagg aggccctgga tgtaattgtg 2341 tccaccttcc acaaatactc aggcaaagag ggtgacaagt tcaagctgaa caagacagag 2401 ctcaaggagc tactgaccag ggagctgcct agcttcctgg ggaaaaggac agatgaagct 2461 gcattccaga aggtgatgag caacttggac agcaacaggg acaatgaagt tgacttccag 2521 gagtactgtg tcttcctgtc ctgcattgcc atgatgtgca atgaattctt tgagggctgc 2581 ccagataagg agccccggaa gaagtgagga tccgcggccg cgaaggatct gcgatcgctc 2641 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 2701 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 2761 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 2821 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 2881 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 2941 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 3001 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 3061 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 3121 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg 3181 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc 3241 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca 3301 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 3361 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 3421 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 3481 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 3541 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 3601 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 3661 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 3721 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca 3781 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 3841 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 3901 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 3961 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4021 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4081 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4141 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 4201 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 4261 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 4321 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 4381 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 4441 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 4501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 4561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 4621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 4681 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 4741 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 4801 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 4861 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 4921 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 4981 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 5041 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 5101 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 5161 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 5221 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 5281 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 5341 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 5401 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 5461 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 5521 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 5581 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 5641 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 5701 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 5761 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 5821 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 5881 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 5941 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 6001 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 6061 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 6121 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 6181 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 6241 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 6301 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 6361 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 6421 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 6481 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 6541 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 6601 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 6661 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 6721 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 6781 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 6841 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 6901 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 6961 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 7021 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 7081 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 7141 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 7201 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 7261 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 7321 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 7381 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 7441 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 7501 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 7561 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 7621 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 7681 agctg //