LOCUS       V034064                12722 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034064
VERSION     V034064
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2288..2297
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2297..2362
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2369..7630
                     /label="Rreb1(NM_001177869)"
                     /note="Rreb1(NM_001177869)"
                     /gene="Rreb1"
     promoter        7692..7903
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7916..8184
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8211..8810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8817..9405
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9479..9712
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9784..9905
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9924..10059
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10097..10113)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10121..10137
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10145..10175)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10190..10211
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10499..11087)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11258..12118)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12119..12223)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12697..12713
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatggact acaaagacca tgacggtgat tataaagatc atgacatcga
     2341 ctacaaggat gacgatgaca aggaattcat gacgtcgaat tcgcccattg gtttagaagg
     2401 ctcagacctg tcttccatca acaccatgat gtcagcagta atgagcgtag cgagtgtcac
     2461 agagaatggt gggagccccc agggcatcaa gtcccccatg aaacctccag gaccaaatcg
     2521 gattggcaga aggaaccagg aaacgaaaga ggagaagtct tcctacaact gtcccctatg
     2581 tgaaaagatt tgcaccaccc agcaccagct gaccatgcac atccgtcagc acaacacaga
     2641 cacgggagga gctgaccacg catgcagtat ctgtgggaag tcgctgagct cggccagctc
     2701 cctggatcgt cacatgctgg tgcactctgg cgagaggcct tacaagtgta cagtgtgtgg
     2761 ccagtctttc accaccaatg ggaacatgca cagacatatg aagattcatg agaaggatac
     2821 caacagtact acagctgcag cccctccatc ccctctgaag cgcaggcggt tgtcctccaa
     2881 aaggaagctg agtcacgatg ccgagtcaga agacccagga ccagctaaaa agatggtaga
     2941 agacgggcag tcaggcgatt tggacaagat gagcgatgaa atctttcact gcccagtgtg
     3001 tttcaaggag tttgtttgca agtatgaact ggagacccac atggagaccc actcagataa
     3061 cccactaaga tgtgacattt gctgcgtcac cttccgcaca catcgaggac tgctgcgcca
     3121 caatgcactt gtccacaagc agcttcccag agatgccatg ggaagacctt ttatccagaa
     3181 caacccttcg attcctgctg gcttccatga tttagggttt actgacttct cctgtaggaa
     3241 gtttcctcga atctctcagg cctggtgtga gacaaaccta cggcggtgca tcagcgagca
     3301 gcaccggttt gtgtgtgaca cctgcgacaa ggcgttcccc atgctgtcgt cactcatcct
     3361 gcacaggcag agccacatcc ctgccgatca gggacgggag aagctccaga ccaagaccct
     3421 ggctgccgag tccttggaac agaaggcctt cctggccttg ctgggcctgc agcacaccaa
     3481 agacgtcaag cctgccccgg cggaggagct cctgccggac gacaaccaag caatacagct
     3541 ccagacactt aagtaccagc tacctcagga gcctggctgc cccaccgtgc tgagtgtgtc
     3601 tcctcttgac gctgcttctt taggtggctc tctgacagtc ctcccagcta ccaaggagaa
     3661 tatgaagcat ctgtccctgc agcccttcca gaagggcttc atcatccagc cagacagtag
     3721 cattgtggtt aagcctattt caggagagtc ggccattgag ctggcagata tccaacagat
     3781 tctaaagatg gcagcttccg ctcctccgca gatcagtctt ccgccacttt ccaaggcccc
     3841 tgccaccccg ctgcaggcga ttttcaagca catgcctcct ttgaagccaa agcccttggt
     3901 cacaccccgg acagtggtag ccgcctccac gcccccacct ctcatcaacg cccagcaggc
     3961 atctcccggt tgtatcagcc ccagccttcc tccacagtcc ctgaagttcc tcaaggggtc
     4021 ggtggaggca gtgtccaatg ttcatctgct tcagtccaag tctgggatcc agccaagcac
     4081 caccacacag ctcttcctgc agcaagctgg agtggagttg ccgggccagc ccgagatgaa
     4141 gacacagctg gaacaagaga gtatcattga ggccctgctg cccctcaaca tggaggcgaa
     4201 gatcaagcag gagataacag aaggtgacct caaggccatc atgacaggcc ccagtggcaa
     4261 gaagaccccc gccatgcgca aggtgctcta cccctgccgc ttttgcaacc aggtgtttgc
     4321 tttctctgga gtcctgcgag cccacgttcg ctcccacctg ggcatctcac cctaccaatg
     4381 caacatctgt gactatattg ctgcagacaa agccgccctg atccgtcaca tccgcacaca
     4441 cagcggggaa cggccttaca tctgcaagat ctgccactac ccattcacgg tcaaagccaa
     4501 ctgtgagcgg cacctgcgca agaagcacct caaggccacc aggaaggaca ttgagaagaa
     4561 catagagtac gtgagcagcc ccaccgcaga gctggtggac gccttctgcg ccccagagac
     4621 cgtgtgcaga ctgtgcgggg aggatctgaa gcactaccga gctctacgca tccacatgcg
     4681 cacacactgc agccggggcc tgggcggctg ccacaaaggc cggaaaccct tcgagtgcaa
     4741 ggaatgcaac gctcccttcg tggccaagcg caactgcatc catcacatcc tcaagcagca
     4801 cctgcacgtg cccgagaagg acatcgagag ctacgtgctt gccaccaaca gtggcctcgg
     4861 ccccgcggac acgcccacgg atgccgcttc cagaggagaa gagggcagct gtgtcacttt
     4921 cgcggagtgc aagcccctcg ccactttcct ggagccccaa aatggctttc ttcactcgag
     4981 ccccacccag cccctgcctt cccacatctc tgtcaagctg gagccagcca gcagctttgc
     5041 catggacttc aacgaacccc ttgacttttc gcagaaaggc ctggcactgg tccaagtgaa
     5101 gcaggaaaat gtttcctcct tgctgacgtc ttcctcctcc tctgccctct atgactgctc
     5161 catggagccc attgacctgt ccatccccaa gagcgtcaag aaaggagaca aggacacagt
     5221 tgttcccagt gacgccaaga aaccggaacc ggaagctggg caagccgagc cgctctcacc
     5281 ccgcccacca ccctgcccta ccttgtcagt gactgtggag cccaagggga gcctggaaac
     5341 ccccacaggc acggtggtgg ccgtcaccac agctgccaag ctagagcccc acactcagcc
     5401 cctccagggc tccgtgcagc tggctgtccc catctactcc cccgcgctcg tcagcaatac
     5461 tcccctcttg ggcaattctg ctgccctctt gaacaaccca gccttgcttc ggcccttacg
     5521 gccaaagccc cccctcctct tgccaaagcc ctcgatgaca gaggagctgc cgccactggc
     5581 ctccatcgcc cagatcattt cttccgtgtc ctcggcccct actctgctga aaacgaaggt
     5641 agctgaccct ggaccatcga tcaccagcag taacactgtg gccacagaca gcccaggaag
     5701 ctccatcccc aaagctgccg ccacccccac tgacaccaca agctctaaag aatccagtga
     5761 gccaccccct gcagccagca gccctgagga agccttgcct actgaacaag ggccggctgc
     5821 cacttcctcg tcgaggaaga ggggcaggaa aaggggactg agaaaccggc ccctccccaa
     5881 cagcagtgct gtggacctgg actccagtgg ggagtttgct agcatcgaga agatgctggc
     5941 caccacagat accaacaagt tcagtccctt tctgcagact gcagaggatg acactcagga
     6001 agaggtggct ggagcccctg ccgaccagca tgggcccgct gatgaggagc aaggtagccc
     6061 cgcagaagac aggctgctga gagcaaagcg gaactcctat gccaactgcc tgcaaaagat
     6121 caactgtccc cactgtcccc gggtcttccc ttgggccagc tccctgcagc ggcacatgct
     6181 tacacacact ggtcagaaac ccttcccttg tcaaaaatgc gatgccttct tttctaccaa
     6241 atctaactgt gagcggcacc agttgcgcaa acacggagtt accacctgct ccctgagaag
     6301 aaacgggctt attcccccaa aagagagtga tgttggatcc catgatagca cagacagcca
     6361 gtcagacaca gacacgttga ccaccccagg tgaggtgctg gatctcacag cacaggctaa
     6421 agagcagccg ccagcagagg gagccagtga gatcagtccg gcttcacagg accttgctat
     6481 caaagaggcc aaagcagcag cagctccctc tgaggaggag gaagaaaagg agacagagga
     6541 gaacccagag ccagaggaag agtgtcgtgt ggaagagagc actggggctg cagatgcccc
     6601 cgaggaagac acagccagca accagagcct ggatcttgac ttcgccacca agctgatgga
     6661 cttcaagctg gcagagagtg aggcaggctc tgtggacagc caaggcccag cccagcagga
     6721 gccaaagcat gcctgcgata cctgtggaaa gaacttcaag ttcctgggca ccctgagtcg
     6781 ccacaagaag gcacacagct gccaggagcc gaaggaggag gaagcagcgg caccttcgct
     6841 ggagaatgag ggcgttggca gagcggttga ggggccctcg ccttcccctg agcctgaaga
     6901 gaaaccagcc gaatccctag caatcgatcc aacaccagga accagggagg cctcagtggc
     6961 gaaacagaat gaagaaacag agggtcccac tgatggggaa ggtacggctg agaagagggg
     7021 tgacggtgac aaaagaccaa agacagactc ccccaaaagc atggccagta aggcagacaa
     7081 gaggaagaag gtttgcagtg tatgcaacaa gcggttctgg tccctgcagg acctcacccg
     7141 gcacatgagg tcacacacag gagaaaggcc atacaagtgt cagacctgtg agcgaacctt
     7201 caccttaaag cacagcctgg ttcgacacca gaggatccac cagaaagcca ggcacagcaa
     7261 gcaccacggg aaagacagcg acaaggatga gcgggctgag gaggacagtg aggacgagtc
     7321 cacccacagt gctaccaacc ctgcctctga gaatgaggcc gagtcagctc ccagcaccag
     7381 caaccacgta gctgtcacca ggagccggaa agagagcttg tccacttctg ggaaggaatg
     7441 tagcccagag gagagagccg cagctgagca ggcagctgag cccagtgccc ccaaagagca
     7501 ggcgtctcca ggtgaaacag acccccagag cccggcagcc atcgtgcaag acttgctgga
     7561 gctgtgcggc aagaggcctg ccccaatcct ggcggccacc gacggggcct cacagctcct
     7621 gggcatggag tgactcgaga gatcagtgta gcggccgcga aggatctgcg atcgctccgg
     7681 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     7741 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     7801 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     7861 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     7921 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     7981 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     8041 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     8101 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     8161 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt
     8221 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     8281 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     8341 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8401 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8461 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8521 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8581 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8641 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8701 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8761 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc
     8821 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8881 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8941 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     9001 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9061 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9121 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9181 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     9241 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9301 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9361 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     9421 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9481 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     9541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9721 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9781 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9841 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9901 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     9961 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10021 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
    10081 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10141 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10921 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    11221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    11281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    11341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    12001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    12061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    12121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    12181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    12241 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    12301 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    12361 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    12421 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    12481 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    12541 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    12601 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    12661 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    12721 tg
//
