LOCUS       V034599                11420 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034599
VERSION     V034599
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5514
                     /label="Rnf31(NM_194346)"
                     /note="Rnf31(NM_194346)"
                     /gene="Rnf31"
     promoter        5579..5790
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5803..6071
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6100..6109
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6130..6792
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6862..6915
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6916..7515
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7516..8103
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8177..8410
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8482..8603
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8622..8757
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8795..8811)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8819..8835
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8843..8873)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8888..8909
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9197..9785)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9956..10816)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10817..10921)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11395..11411
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cgggagacga ggagcgaggc
     2341 ttcctggcgg cccgcgagga gctggcgagc gccctgaggt gggattctgc gcaggttttt
     2401 cccctggagc agctcatgcc gcttctggcc acctctctgc caccagccgc ccgctacctg
     2461 cagctggacg ccggacgctt ggtccgctgc aacgctcatg gggagcctcg aaactacctc
     2521 aacaccctat ccacggccct gaacatcctg gaaaaatatg gtcgcaacct cctcagcccg
     2581 cagcggcccc ggtattggcg ctcagtgaag tttaataacc ccgtctttcg cagcacggtg
     2641 gatgctgtgc agggtggccg ggatgtacta cggttgtatg gctatactga ggagcgccca
     2701 gatggattga gtttccccga agggcaggag gaaccagatg aataccaggt tgctgttgtc
     2761 acactagaag tactgctgct tcgcaccgag ctcagtttgc tgttgcagaa tactcatccc
     2821 agacagaatg cactggacca gctgctaaga gagagcgttg aagatggtat gctgcagctt
     2881 tcagagtttc acccccttct gagggagatt gttcctggcc cccgcccctc tgcccaaggc
     2941 tccactcctg gtccctgttt cctctgtggt tctgccccag gcacactgca ctgtccagcc
     3001 tgtaaccaag tctcgtgccc agcttgtgac attttgttcc atgggcatcc gtcccgtgca
     3061 catcaccttc gccaagccct gcctgggtcc caccagactg ccagcctgag ctctagttta
     3121 cctgcctcgt cccaaccacg gcccccctcc tcctccttgg ccctgggaga tagctctctt
     3181 tcttcccctg accctgcaaa tgcctgtctg ccctggcatt gtcttacctg tgccacacta
     3241 aatgagcctt gggcagtgtt ctgtgcagtc tgtagtcagc ccaaaggctg caaggtgccg
     3301 ggaatagagg gttcccatgg aaccgggggc ctagaacctg agcctgcacg ggatcaatgg
     3361 gcctgccaga gctgtacctt tgagaatgag gcagcagctg tgctatgcgc catatgtgag
     3421 cgacctcggc tggcccagcc tcccagcttg gtggtggatt cccatgatgc tggtgtttgc
     3481 caacagtccc ttaagcagga ggatcctttg ctcaccgctg cccagcctca ggtgtggtac
     3541 tgtgaccatt gtaccttctg caattcaggc cctgtctggg tgtgtgccat gtgcaaccga
     3601 acccgagacc ccatccctac acagcctgcc ctccagtcct atcccagctc tttggaaaag
     3661 ggacgcccaa agccagggtc ctcacaacac cttggttcct ccctgcctgc ttcctgtgga
     3721 gacccagaga aacaacgcca agataagatg cggaaggaag gtctccagct cgtgagcatg
     3781 atccaggaag gagaaactgc gggtgccagt ccagaagagg tcttctcagc tctccaatac
     3841 tcaggcacag aggtgcccct ccagtggttg cgttcagagc tgtcctacgt cctggagatg
     3901 gtggctgagc ttgctggaca acaggatcca gagctggggg ccttttcctg tcaggaagcc
     3961 cggaaagcct ggcttgatcg ccatggcaac ctggatgaag ctgtagagga gtgtgtgagg
     4021 gccaggagga ggaaggtgca cgagctgcag tccctgggct ttgggcctaa ggaagggtca
     4081 ctacaggcat tgttccagca tgggggtgac gtggctcggg ccctgactga gttacagcgc
     4141 cagcgcctgg agcccttcca tcagcgccta tgggacagag accctgaacc cactccctgc
     4201 tgggatgggc tggacagaca gagcttggtc agacgccttc tggccgtcta cacactcccc
     4261 agctggggcc gagcagagct ggcgctggcg ctgctgcagg agacacccag gaactatgag
     4321 ttgttggacg tggtggaggc tgtgaggcac agccaggacc gggcctttct gcgtcgactg
     4381 cttgcccagg aatgtgctgt gtgcgggtgg gcccttcccc gcaaccggat gcaggccctg
     4441 atctcctgtg agtgcaccat atgtcccgaa tgcttccgcc aacacttcac cattgccctg
     4501 aaggagaagc acatcacaga catggtgtgc cctgcctgtg gccgccctga cctcactgat
     4561 gacgctcagt tactcagcta cttctccacc cttgacatcc agctcagaga gagcctagac
     4621 cccgatgcat atgccctgtt tcacaagaag ctgaccgagg ctgtgcttat gcgagacccc
     4681 aagttcttgt ggtgcgccca gtgttccttt ggcttcatct atgaacgcga acagctggag
     4741 gcgacgtgtc cccagtgtca ccagaccttc tgtgtgcgct gcaagcgcca gtgggaggag
     4801 cagcacagag gacggagttg tgaggatttc cagaactgga aacgcaccaa tgacccagag
     4861 taccaggctc aaggcttggc catgtacctt caggaaaacg gcattgactg tccgaaatgc
     4921 aagttctcgt acgcactggc ccggggaggc tgcatgcact tccactgcac gcagtgtcga
     4981 caccagttct gcagtggctg ctacaacgcc ttttacgcca agaataaatg tccagaccct
     5041 aactgcaagg tgaaaaagtc cctgcatggc caccaccctc gagactgcct cttctaccta
     5101 cgggactgga ctgctgcccg cctccagaaa ctgttgcagg acaataatgt catgtttaat
     5161 acagagcctc cagctgggac acgggcagtc cctggagggg gctgcagagt gatggagcag
     5221 aaggaggtcc atagtgggtt cagggatgaa gcttgcggca aggaaactcc acctggctat
     5281 gccggcctat gtcaggcaca ctacaaagag tatctcgtga gcctcatcaa tgcccattca
     5341 ctggacccag ctaccttgta tgaagtggag gagctggaga cagccactat tcgctaccta
     5401 catttagctc ctcagcccgc ggatggagag gatctgcctg cttaccaggc ccggctatta
     5461 cagaagctga gagaagaggt acccttggga cagagtattg cccgcagaag aaagtaggga
     5521 tccctgcgat atccagcgcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     5581 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5641 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5701 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5761 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5821 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5881 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5941 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     6001 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     6061 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     6121 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     6181 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     6241 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     6301 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     6361 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     6421 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     6481 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     6541 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     6601 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     6661 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     6721 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     6781 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     6841 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6901 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6961 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7021 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7081 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7141 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7201 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7261 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7321 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7381 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7441 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7501 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7561 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7621 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7681 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7741 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7801 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7861 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     7921 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7981 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8041 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc
     8101 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8161 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     8221 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8281 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8341 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8401 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     8461 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     8521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8581 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     8641 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8701 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8761 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     8821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     9661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10921 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    10981 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    11041 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    11101 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    11161 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    11221 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    11281 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    11341 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    11401 cgacggccag tgccaagctg
//
