LOCUS       V025037                10910 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025037
VERSION     V025037
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4953
                     /label="Rapgef5(NM_001373989)"
                     /note="Rapgef5(NM_001373989)"
                     /gene="Rapgef5"
     CDS             4960..5025
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5069..5280
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5293..5561
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5590..5599
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5620..6282
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6352..6405
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6406..7005
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7006..7593
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7667..7900
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7972..8093
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8112..8247
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8285..8301)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8309..8325
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8333..8363)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8378..8399
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8687..9275)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9446..10306)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10307..10411)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10885..10901
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggtgggctc tgtcaagatg
     2341 cagccgcctt gcgagagtcc ggccctggcc tcggcggccc cggccgatgg ccctctgcgc
     2401 cgcagcccga gcgcccggga gccggagcgc gagccgcccg ccgcgtctct gcggccgcgc
     2461 ctcagggacc tgcctgcgct gctgcggagc gggctcacgc tgcggaggaa gcggagcgcc
     2521 gccggcgccc ggactctctc aagaagaatt tctaatccat accttgaaca tagtccttct
     2581 cagatttatg gagagaattc ttcctgtgca ggaagagcat taaggaatat tattatcctt
     2641 caagcagctg acctggtaaa ggacagagtg aatctcaagg ggttttacag gagaagctgt
     2701 gtggggtcag agctggtcga ctggctcctg gaacactgtc cttttgtcca gtgtagatct
     2761 atggccattg gagtctggca gctcctactg gatatgggga ttatgtcatc cgtggaccaa
     2821 catctatact ttcaagataa ttatgttttc taccagtttt cttcggatga gtgcagctac
     2881 ttgtactgcg aatttgaaag agaggaagaa tggcagaagg gagtgaagct attgctggag
     2941 cttgtgcatc tcattcctgc aagagctggc atctgtgacc tgtctcacca gaaaacggaa
     3001 gactcggagg aaagcagtga tgagattctt gctcggctaa catctgcggt gcagagagag
     3061 ctggcggctg ttattgcttt gaaagcaaga aagtctgcaa ttgagcaaga tgatgaaaac
     3121 gctgacaagc atgtaaccgt gacggaggct aacaacggtc ccgatcctca ggctggggtg
     3181 atgtgcaagc ttcaagagag agatgatatc ggacgtattg agctggtcca caagctggca
     3241 agagaaaact gtcagttttt acagacagaa aaaaaagaat cggagaagtt ggaacagcaa
     3301 gatgatgaag tgacgatggt tcaggttaaa gagcaaggcc agagtgtgct ggtgctgaag
     3361 aaagtagcaa gctgtggccc agcccccacc tcagggagtg cggagaacga cgcgagatat
     3421 gtggtggtgt ctggtacccc ggagaagatc ttggagcacc ttttgaatga cttgcacctg
     3481 gcagaggttc agcacaaaga aacagaaaca ctccttgatg acttcctcct cacgtacacc
     3541 gtattcatga caactgatga cttgtgccag gcgctgctaa gacactattc tgccaagaag
     3601 tatcaagggg aagaagaaaa ctcagatgtc ccttgtcgga agcgcaaggt cttacatctg
     3661 gtttcccagt ggatttctct gtacaaggac tggctacatg aagatgagca ttcaaaaatg
     3721 tttctaaaga ccatatacag gaacgtgctg gatgatgtct atgagtaccc gattcttgag
     3781 aaagagctga aagagtttca gaagatactt ggcgtgtacc gccgtcacac tgttgatgaa
     3841 tactcaccac agaagaagaa taaagccctt ttccaccaat tcagtcttaa ggagaactgg
     3901 ctccagcaca gaggaactgt ggcagaaaca gaggaaattt tttgccacgt gtatataacg
     3961 gagcactcct atatcagtgt gaaggcaaaa gtgtcgagta cagcccagga gatcctgaag
     4021 gtcgtggccg agaagctgca gcgtgccgaa gaggatctgg ctctggtagc cattacattc
     4081 tctggagaaa agcatgaatt tcagccaaat gacttggcca tttccaaatc cctggaggca
     4141 tctggtcgga tatatgtcta ccgaaaagat ctggctgaca ctttgaatcc tttggcagaa
     4201 aatgaggaat cccagcaaag gtcaatgagg attttgggga tgaacacgtg ggaccttgct
     4261 ctggaattaa tgagttttga ttggagtctg ttcaattcaa tccacgagca agagctgatc
     4321 tacttcacat tcagcagaca ggggaacgga gagaatactg tgaacctcag ccttctgctc
     4381 cagagatgta acgaggtcca gctctgggta gccacagaga ttctgctctg cagccagcta
     4441 ggcaagcgcg tgcagctggt gaaaaaattc atcaagattg cagctcactg caaagcccaa
     4501 cagaacctaa actctttctt tgccatcgtg atgggtctca acaccgcctc tgtcagccgg
     4561 ctatcacaga cctgggagaa aatccctggg aagtttaaga agcttttctc tgaacttgag
     4621 agtttaacgg atccttccct aaaccacaaa gcctacaggg atgcgttcaa aaagatgaag
     4681 ccaccaaaaa tccctttcat gcccttactg cttaaagatg taacgtttat tcatgaagga
     4741 aataaaacat tcttggataa tctcgtcaat tttgaaaagc tgcacatgat tgcagacact
     4801 gtccgaaccc tgagacactg caggactaac cagtttggaa gtgacgtgtc tccaaaggag
     4861 cagcaagagc tgaagtccta tgtcaaccac ctgtatgtca tcgacagcca gcaagccctg
     4921 ttcgagctct ctcacaggct ggagcctcga gccggatccg actacaaaga ccatgacggt
     4981 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5041 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5101 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5161 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5221 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5281 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5341 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5401 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5461 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5521 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5581 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5641 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5701 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5761 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5821 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5881 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5941 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6001 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6061 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6121 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6181 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6241 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6301 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6361 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6421 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6481 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6541 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6601 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6661 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6721 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6781 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6841 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6901 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6961 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7021 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7081 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7141 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7201 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7261 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7321 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7381 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7441 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7501 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag
     7561 acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     7621 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7681 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7741 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7801 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7861 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7921 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8101 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8161 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8221 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     8281 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8341 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8401 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8461 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9121 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10441 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10501 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    10561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10621 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10681 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10741 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10801 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10861 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
