LOCUS V039269 9122 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039269 VERSION V039269 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3954 /label="RELA(NM_021975)" /note="RELA(NM_021975)" /gene="RELA" CDS 3961..3987 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3991..4017 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4021..4047 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4092..4303 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4316..4584 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4611..5210 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5217..5805 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5879..6112 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6184..6305 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6324..6459 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6497..6513) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6521..6537 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6545..6575) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6590..6611 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6899..7487) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7658..8518) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8519..8623) /gene="bla" /label="AmpR promoter" primer_bind 9097..9113 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggacgaa ctgttccccc tcatcttccc ggcagagcca 2341 gcccaggcct ctggccccta tgtggagatc attgagcagc ccaagcagcg gggcatgcgc 2401 ttccgctaca agtgcgaggg gcgctccgcg ggcagcatcc caggcgagag gagcacagat 2461 accaccaaga cccaccccac catcaagatc aatggctaca caggaccagg gacagtgcgc 2521 atctccctgg tcaccaagga ccctcctcac cggcctcacc cccacgagct tgtaggaaag 2581 gactgccggg atggcttcta tgaggctgag ctctgcccgg accgctgcat ccacagtttc 2641 cagaacctgg gaatccagtg tgtgaagaag cgggacctgg agcaggctat cagtcagcgc 2701 atccagacca acaacaaccc cttccaagtt cctatagaag agcagcgtgg ggactacgac 2761 ctgaatgctg tgcggctctg cttccaggtg acagtgcggg acccatcagg caggcccctc 2821 cgcctgccgc ctgtcctttc tcatcccatc tttgacaatc gtgcccccaa cactgccgag 2881 ctcaagatct gccgagtgaa ccgaaactct ggcagctgcc tcggtgggga tgagatcttc 2941 ctactgtgtg acaaggtgca gaaagaggac attgaggtgt atttcacggg accaggctgg 3001 gaggcccgag gctccttttc gcaagctgat gtgcaccgac aagtggccat tgtgttccgg 3061 acccctccct acgcagaccc cagcctgcag gctcctgtgc gtgtctccat gcagctgcgg 3121 cggccttccg accgggagct cagtgagccc atggaattcc agtacctgcc agatacagac 3181 gatcgtcacc ggattgagga gaaacgtaaa aggacatatg agaccttcaa gagcatcatg 3241 aagaagagtc ctttcagcgg acccaccgac ccccggcctc cacctcgacg cattgctgtg 3301 ccttcccgca gctcagcttc tgtccccaag ccagcacccc agccctatcc ctttacgtca 3361 tccctgagca ccatcaacta tgatgagttt cccaccatgg tgtttccttc tgggcagatc 3421 agccaggcct cggccttggc cccggcccct ccccaagtcc tgccccaggc tccagcccct 3481 gcccctgctc cagccatggt atcagctctg gcccaggccc cagcccctgt cccagtccta 3541 gccccaggcc ctcctcaggc tgtggcccca cctgccccca agcccaccca ggctggggaa 3601 ggaacgctgt cagaggccct gctgcagctg cagtttgatg atgaagacct gggggccttg 3661 cttggcaaca gcacagaccc agctgtgttc acagacctgg catccgtcga caactccgag 3721 tttcagcagc tgctgaacca gggcatacct gtggcccccc acacaactga gcccatgctg 3781 atggagtacc ctgaggctat aactcgccta gtgacagggg cccagaggcc ccccgaccca 3841 gctcctgctc cactgggggc cccggggctc cccaatggcc tcctttcagg agatgaagac 3901 ttctcctcca ttgcggacat ggacttctca gccctgctga gtcagatcag ctccggatcc 3961 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc 4021 tatccttatg atgtcccaga ctacgcatag gcggccgcga aggatctgcg atcgctccgg 4081 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 4141 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 4201 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 4261 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 4321 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 4381 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 4441 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 4501 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 4561 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt 4621 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg 4681 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg 4741 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4801 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4861 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 4921 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4981 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 5041 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 5101 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 5161 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc 5221 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5281 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5341 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5401 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5461 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5521 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5581 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 5641 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5701 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5761 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 5821 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 5881 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 5941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 6121 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6181 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6301 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 6361 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 6421 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 6481 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6541 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6601 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6661 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7321 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7621 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7681 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7741 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7801 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7861 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7921 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7981 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8041 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8101 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8161 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8221 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8281 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8341 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8401 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8461 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8521 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8581 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8641 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 8701 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8761 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8821 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 8881 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 8941 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 9001 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 9061 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 9121 tg //