LOCUS       V021387                11009 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021387
VERSION     V021387
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2832..5336
                     /label="Tlr4(NM_021297)"
                     /note="Tlr4(NM_021297)"
                     /gene="Tlr4"
     CDS             5343..5399
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5400..6110
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        6141..6470
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6480..7079
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7146..7734
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7821..8054
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8126..8247
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8287..8422
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8443..8461)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8471..8487)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8629..9084
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9110..9214
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9215..10075
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10246..10834
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagacgg
     2821 gatccgccac catgatgcct ccctggctcc tggctaggac tctgatcatg gcactgttct
     2881 tctcctgcct gacaccagga agcttgaatc cctgcataga ggtagttcct aatattacct
     2941 accaatgcat ggatcagaaa ctcagcaaag tccctgatga cattccttct tcaaccaaga
     3001 acatagatct gagcttcaac cccttgaaga tcttaaaaag ctatagcttc tccaattttt
     3061 cagaacttca gtggctggat ttatccaggt gtgaaattga aacaattgaa gacaaggcat
     3121 ggcatggctt acaccacctc tcaaacttga tactgacagg aaaccctatc cagagttttt
     3181 ccccaggaag tttctctgga ctaacaagtt tagagaatct ggtggctgtg gagacaaaat
     3241 tggcctctct agaaagcttc cctattggac agcttataac cttaaagaaa ctcaatgtgg
     3301 ctcacaattt tatacattcc tgtaagttac ctgcatattt ttccaatctg acgaacctag
     3361 tacatgtgga tctttcttat aactatattc aaactattac tgtcaacgac ttacagtttc
     3421 tacgtgaaaa tccacaagtc aatctctctt tagacatgtc tttgaaccca attgacttca
     3481 ttcaagacca agcctttcag ggaattaagc tccatgaact gactctaaga ggtaatttta
     3541 atagctcaaa tataatgaaa acttgccttc aaaacctggc tggtttacac gtccatcggt
     3601 tgatcttggg agaatttaaa gatgaaagga atctggaaat ttttgaaccc tctatcatgg
     3661 aaggactatg tgatgtgacc attgatgagt tcaggttaac atatacaaat gatttttcag
     3721 atgatattgt taagttccat tgcttggcga atgtttctgc aatgtctctg gcaggtgtat
     3781 ctataaaata tctagaagat gttcctaaac atttcaaatg gcaatcctta tcaatcatta
     3841 gatgtcaact taagcagttt ccaactctgg atctaccctt tcttaaaagt ttgactttaa
     3901 ctatgaacaa agggtctatc agttttaaaa aagtggccct accaagtctc agctatctag
     3961 atcttagtag aaatgcactg agctttagtg gttgctgttc ttattctgat ttgggaacaa
     4021 acagcctgag acacttagac ctcagcttca atggtgccat cattatgagt gccaatttca
     4081 tgggtctaga agagctgcag cacctggatt ttcagcactc tactttaaaa agggtcacag
     4141 aattctcagc gttcttatcc cttgaaaagc tactttacct tgacatctct tatactaaca
     4201 ccaaaattga cttcgatggt atatttcttg gcttgaccag tctcaacaca ttaaaaatgg
     4261 ctggcaattc tttcaaagac aacacccttt caaatgtctt tgcaaacaca acaaacttga
     4321 cattcctgga tctttctaaa tgtcaattgg aacaaatatc ttggggggta tttgacaccc
     4381 tccatagact tcaattatta aatatgagtc acaacaatct attgtttttg gattcatccc
     4441 attataacca gctgtattcc ctcagcactc ttgattgcag tttcaatcgc atagagacat
     4501 ctaaaggaat actgcaacat tttccaaaga gtctagcctt cttcaatctt actaacaatt
     4561 ctgttgcttg tatatgtgaa catcagaaat tcctgcagtg ggtcaaggaa cagaagcagt
     4621 tcttggtgaa tgttgaacaa atgacatgtg caacacctgt agagatgaat acctccttag
     4681 tgttggattt taataattct acctgttata tgtacaagac aatcatcagt gtgtcagtgg
     4741 tcagtgtgat tgtggtatcc actgtagcat ttctgatata ccacttctat tttcacctga
     4801 tacttattgc tggctgtaaa aagtacagca gaggagaaag catctatgat gcatttgtga
     4861 tctactcgag tcagaatgag gactgggtga gaaatgagct ggtaaagaat ttagaagaag
     4921 gagtgccccg ctttcacctc tgccttcact acagagactt tattcctggt gtagccattg
     4981 ctgccaacat catccaggaa ggcttccaca agagccggaa ggttattgtg gtagtgtcta
     5041 gacactttat tcagagccgt tggtgtatct ttgaatatga gattgctcaa acatggcagt
     5101 ttctgagcag ccgctctggc atcatcttca ttgtccttga gaaggttgag aagtccctgc
     5161 tgaggcagca ggtggaattg tatcgccttc ttagcagaaa cacctacctg gaatgggagg
     5221 acaatcctct ggggaggcac atcttctgga gaagacttaa aaatgcccta ttggatggaa
     5281 aagcctcgaa tcctgagcaa acagcagagg aagaacaaga aacggcaact tggaccctcg
     5341 aggctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta
     5401 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     5461 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     5521 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     5581 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     5641 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     5701 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     5761 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     5821 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     5881 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     5941 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     6001 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     6061 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa cgcgtcgacc
     6121 agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca
     6481 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6541 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6601 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6661 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6721 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6781 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6841 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6901 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6961 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7021 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     7081 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgccag
     7141 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7201 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7261 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7321 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7381 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7441 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7501 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7561 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7621 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7681 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7741 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7801 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7861 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7921 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7981 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8041 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8101 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8161 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8221 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8281 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8341 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8401 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     8461 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8521 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8581 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     8641 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     8701 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     8761 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     8821 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     8881 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8941 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9001 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     9061 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9181 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9241 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9301 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9361 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9421 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     9481 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9541 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9601 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9661 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9721 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9781 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9841 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9901 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9961 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10021 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10081 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    10321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10381 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    10441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    10501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10981 cgagcgcagc gagtcagtga gcgaggaag
//
