LOCUS       V025915                10042 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025915
VERSION     V025915
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2818..2827
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2827..2898
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2899..4368
                     /label="TFEB(NM_001167827)"
                     /note="TFEB(NM_001167827)"
                     /gene="TFEB"
     CDS             4405..5124
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5174..5503
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5513..6112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6179..6767
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6854..7087
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7159..7280
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7320..7455
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(7476..7494)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(7504..7520)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      7662..8117
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8143..8247
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8248..9108
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9279..9867
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagcc
     2821 accatggact acaaggatga cgatgacaag gattacaaag acgacgatga taaggactat
     2881 aaggatgatg acgacaaaat gacagcaagc tcaggctggg agccagcgcc ggcagccacc
     2941 atggcgtcac gcatagggtt gcgcatgcag ctcatgcggg agcaggcgca gcaggaggag
     3001 cagcgggagc gcatgcagca acaggctgtc atgcattaca tgcagcagca gcagcagcag
     3061 caacagcagc agctcggagg gccgcccacc ccggccatca atacccccgt ccacttccag
     3121 tcgccaccac ctgtgcctgg ggaggtgttg aaggtgcagt cctacctgga gaatcccaca
     3181 tcctaccatc tgcagcagtc gcagcatcag aaggtgcggg agtacctgtc cgagacctat
     3241 gggaacaagt ttgctgccca catcagccca gcccagggct ctccgaaacc cccaccagcc
     3301 gcctccccag gggtgcgagc tggacacgtg ctgtcctcct ccgctggcaa cagtgctccc
     3361 aatagcccca tggccatgct gcacattggc tccaaccctg agagggagtt ggatgatgtc
     3421 attgacaaca ttatgcgtct ggacgatgtc cttggctaca tcaatcctga aatgcagatg
     3481 cccaacacgc tacccctgtc cagcagccac ctgaatgtgt acagcagcga cccccaggtc
     3541 acagcctccc tggtgggcgt caccagcagc tcctgccctg cggacctgac ccagaagcga
     3601 gagctcacag atgctgagag cagggccctg gccaaggagc ggcagaagaa agacaatcac
     3661 aacttaattg aaaggagacg aaggttcaac atcaatgacc gcatcaagga gttgggaatg
     3721 ctgatcccca aggccaatga cctggacgtg cgctggaaca agggcaccat cctcaaggcc
     3781 tctgtggatt acatccggag gatgcagaag gacctgcaaa agtccaggga gctggagaac
     3841 cactctcgcc gcctggagat gaccaacaag cagctctggc tccgtatcca ggagctggag
     3901 atgcaggctc gagtgcacgg cctccctacc acctccccgt ccggcatgaa catggctgag
     3961 ctggcccagc aggtggtgaa gcaggagctg cctagcgaag agggcccagg ggaggccctg
     4021 atgctggggg ctgaggtccc tgaccctgag ccactgccag ctctgccccc gcaagccccg
     4081 ctgcccctgc ccacccagcc accatcccca ttccatcacc tggacttcag ccacagcctg
     4141 agctttgggg gcagggagga cgagggtccc ccgggctacc ccgaacccct ggcgccgggg
     4201 catggctccc cattccccag cctgtccaag aaggatctgg acctcatgct cctggacgac
     4261 tcactgctac cgctggcctc tgatccactt ctgtccacca tgtcccccga ggcctccaag
     4321 gccagcagcc gccggagcag cttcagcatg gaggagggcg atgtgctgct cgagggcggt
     4381 ggaggcagtg gtggaggcgg tagcatggtg agcaagggcg aggagctgtt caccggggtg
     4441 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4501 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4561 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4621 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4681 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4741 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4801 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4861 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4921 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4981 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5041 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5101 ggcatggacg agctgtacaa gtaacgggat ccggactagt taacgcgtcg accagtggtc
     5161 gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5221 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5281 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga
     5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5821 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5881 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6121 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgc cagtcgacaa
     6181 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6241 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6301 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6361 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6421 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6481 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6541 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6601 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6661 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6721 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc
     6781 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6841 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6901 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6961 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7021 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7081 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7141 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7261 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7441 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     7501 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     7561 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7621 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     7681 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7741 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7801 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7861 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7921 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7981 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8041 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8101 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8161 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8221 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8281 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8341 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8401 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8461 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8521 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8581 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8641 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8701 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8761 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8821 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8881 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8941 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9001 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9061 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9121 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9181 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9421 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10021 agcgagtcag tgagcgagga ag
//
