LOCUS       V045277                11107 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045277
VERSION     V045277
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2824..5433
                     /label="EPAS1(NM_001430)"
                     /note="EPAS1(NM_001430)"
                     /gene="EPAS1"
     CDS             5470..6189
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        6239..6568
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6578..7177
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7244..7832
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7919..8152
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8224..8345
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8385..8520
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8541..8559)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8569..8585)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8727..9182
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9208..9312
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9313..10173
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10344..10932
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagcc
     2821 accatgacag ctgacaagga gaagaaaagg agtagctcgg agaggaggaa ggagaagtcc
     2881 cgggatgctg cgcggtgccg gcggagcaag gagacggagg tgttctatga gctggcccat
     2941 gagctgcctc tgccccacag tgtgagctcc catctggaca aggcctccat catgcgactg
     3001 gcaatcagct tcctgcgaac acacaagctc ctctcctcag tttgctctga aaacgagtcc
     3061 gaagccgaag ctgaccagca gatggacaac ttgtacctga aagccttgga gggtttcatt
     3121 gccgtggtga cccaagatgg cgacatgatc tttctgtcag aaaacatcag caagttcatg
     3181 ggacttacac aggtggagct aacaggacat agtatctttg acttcactca tccctgcgac
     3241 catgaggaga ttcgtgagaa cctgagtctc aaaaatggct ctggttttgg gaaaaaaagc
     3301 aaagacatgt ccacagagcg ggacttcttc atgaggatga agtgcacggt caccaacaga
     3361 ggccgtactg tcaacctcaa gtcagccacc tggaaggtct tgcactgcac gggccaggtg
     3421 aaagtctaca acaactgccc tcctcacaat agtctgtgtg gctacaagga gcccctgctg
     3481 tcctgcctca tcatcatgtg tgaaccaatc cagcacccat cccacatgga catccccctg
     3541 gatagcaaga ccttcctgag ccgccacagc atggacatga agttcaccta ctgtgatgac
     3601 agaatcacag aactgattgg ttaccaccct gaggagctgc ttggccgctc agcctatgaa
     3661 ttctaccatg cgctagactc cgagaacatg accaagagtc accagaactt gtgcaccaag
     3721 ggtcaggtag taagtggcca gtaccggatg ctcgcaaagc atgggggcta cgtgtggctg
     3781 gagacccagg ggacggtcat ctacaaccct cgcaacctgc agccccagtg catcatgtgt
     3841 gtcaactacg tcctgagtga gattgagaag aatgacgtgg tgttctccat ggaccagact
     3901 gaatccctgt tcaagcccca cctgatggcc atgaacagca tctttgatag cagtggcaag
     3961 ggggctgtgt ctgagaagag taacttccta ttcaccaagc taaaggagga gcccgaggag
     4021 ctggcccagc tggctcccac cccaggagac gccatcatct ctctggattt cgggaatcag
     4081 aacttcgagg agtcctcagc ctatggcaag gccatcctgc ccccgagcca gccatgggcc
     4141 acggagttga ggagccacag cacccagagc gaggctggga gcctgcctgc cttcaccgtg
     4201 ccccaggcag ctgccccggg cagcaccacc cccagtgcca ccagcagcag cagcagctgc
     4261 tccacgccca atagccctga agactattac acatctttgg ataacgacct gaagattgaa
     4321 gtgattgaga agctcttcgc catggacaca gaggccaagg accaatgcag tacccagacg
     4381 gatttcaatg agctggactt ggagacactg gcaccctata tccccatgga cggggaagac
     4441 ttccagctaa gccccatctg ccccgaggag cggctcttgg cggagaaccc acagtccacc
     4501 ccccagcact gcttcagtgc catgacaaac atcttccagc cactggcccc tgtagccccg
     4561 cacagtccct tcctcctgga caagtttcag cagcagctgg agagcaagaa gacagagccc
     4621 gagcaccggc ccatgtcctc catcttcttt gatgccggaa gcaaagcatc cctgccaccg
     4681 tgctgtggcc aggccagcac ccctctctct tccatggggg gcagatccaa tacccagtgg
     4741 cccccagatc caccattaca ttttgggccc acaaagtggg ccgtcgggga tcagcgcaca
     4801 gagttcttgg gagcagcgcc gttggggccc cctgtctctc caccccatgt ctccaccttc
     4861 aagacaaggt ctgcaaaggg ttttggggct cgaggcccag acgtgctgag tccggccatg
     4921 gtagccctct ccaacaagct gaagctgaag cgacagctgg agtatgaaga gcaagccttc
     4981 caggacctga gcggggggga cccacctggt ggcagcacct cacatttgat gtggaaacgg
     5041 atgaagaacc tcaggggtgg gagctgccct ttgatgccgg acaagccact gagcgcaaat
     5101 gtacccaatg ataagttcac ccaaaacccc atgaggggcc tgggccatcc cctgagacat
     5161 ctgccgctgc cacagcctcc atctgccatc agtcccgggg agaacagcaa gagcaggttc
     5221 cccccacagt gctacgccac ccagtaccag gactacagcc tgtcgtcagc ccacaaggtg
     5281 tcaggcatgg caagccggct gctcgggccc tcatttgagt cctacctgct gcccgaactg
     5341 accagatatg actgtgaggt gaacgtgccc gtgctgggaa gctccacgct cctgcaagga
     5401 ggggacctcc tcagagccct ggaccaggcc accctcgagg gcggtggagg cagtggtgga
     5461 ggcggtagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5521 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5581 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     5641 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     5701 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5761 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5821 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     5881 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     5941 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6001 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6061 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6121 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6181 tacaagtaac gggatccgga ctagttaacg cgtcgaccag tggtcgaccc tgtggaatgt
     6241 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6301 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6361 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6541 cttttttgga ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt
     6601 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6661 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6721 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6781 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6841 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6901 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6961 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7021 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7081 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7141 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     7201 cccgaccgaa aggagcgcac gaccccatgc atcgccagtc gacaatcaac ctctggatta
     7261 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7321 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7381 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7441 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7501 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7561 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7621 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7681 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7741 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7801 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga
     7861 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7921 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7981 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8041 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8101 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8161 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8221 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8281 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8341 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     8401 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8461 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8521 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8581 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8641 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8701 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     8761 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8821 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8881 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8941 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9001 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9061 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9121 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9181 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9241 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9301 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9361 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9421 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9481 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9541 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9601 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9661 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9721 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9781 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9841 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9901 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9961 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10021 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10081 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10141 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10201 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10261 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10321 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10381 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10441 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10501 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10561 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10621 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10681 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10741 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10801 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10861 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10921 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10981 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11041 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11101 gaggaag
//
