LOCUS       V030813                10045 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030813
VERSION     V030813
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4087
                     /label="Ptpn11(NM_011202)"
                     /note="Ptpn11(NM_011202)"
                     /gene="Ptpn11"
     CDS             4094..4159
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4204..4415
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4428..4696
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4725..4734
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4755..5417
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5487..5540
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5541..6140
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6141..6728
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6802..7035
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7107..7228
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7247..7382
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7420..7436)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7444..7460
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7468..7498)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7513..7534
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7822..8410)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8581..9441)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9442..9546)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10020..10036
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga catcgcggag atggtttcac cccaacatca ctggtgtgga
     2341 ggcagagaat ctcctgctga ccagaggagt cgatggcagt tttttagcaa ggcccagtaa
     2401 gagtaaccct ggagacttca ctctgtctgt tagaagaaat ggagctgtta cccacatcaa
     2461 gattcagaac actggggact actatgacct ctatggtggg gagaagtttg ccactttggc
     2521 tgaactggtt cagtattaca tggaacacca tgggcagctg aaagagaaga atggagatgt
     2581 tatcgagctc aagtacccgc tgaactgtgc agaccctacc tctgaaaggt ggttccatgg
     2641 tcacttgtct ggaaaagaag cagagaagct gctgacggag aagggcaagc atggcagctt
     2701 cctcgttcga gagagccaga gccaccccgg agacttcgtt ctctccgtgc gcactggtga
     2761 cgacaaaggg gagagcaacg acggcaagtc caaagtgacc cacgtcatga tccgctgtca
     2821 ggagctgaaa tacgacgttg gtgggggaga gcgctttgac tctctgacag acctggtgga
     2881 gcattacaag aagaacccca tggtggagac gctgggcaca gtcctgcagc tcaagcagcc
     2941 cctcaacaca actcgtatca atgctgctga aattgaaagc cgggttcgag agttaagcaa
     3001 gctggctgag accacagata aagtcaagca gggcttttgg gaagagtttg agacgctcca
     3061 gcaacaggaa tgcaaacttc tctatagccg aaaagaagga cagagacaag aaaataaaaa
     3121 caaaaacaga tacaaaaaca tcctgccctt tgatcatacc agggtcgttc tgcatgatgg
     3181 ggatcccaat gagcctgttt ctgattacat taatgcaaac atcatcatgc ctgagtttga
     3241 gaccaagtgc aacaattcca aacccaaaaa gagttacatt gccactcaag gctgcctgca
     3301 gaacacggtg aatgacttct ggcggatggt gttccaggag aactctcgag tcattgtcat
     3361 gaccacaaag gaagtggaga gagggaagag caaatgtgtc aagtactggc ctgatgagta
     3421 tgcgctcaaa gaatacgggg tcatgcgtgt taggaacgtc aaagaaagtg ccgcccatga
     3481 ctacacttta cgagagctca aactctctaa ggtcggacaa gctctactcc agggaaacac
     3541 agagagaacc gtctggcagt accactttcg gacctggcca gaccatggcg tgcctagtga
     3601 ccctggaggt gtgctggact tcctggagga ggtccaccac aagcaggaga gcatcgtgga
     3661 tgcaggccct gtcgtggttc actgcagcgc tgggattggc cggacaggaa ccttcattgt
     3721 gattgacatc cttattgaca tcattcgaga gaaaggtgtg gactgtgaca tcgacgttcc
     3781 taaaaccatt cagatggtgc ggtcccagag gtcggggatg gtccagacag aagcacagta
     3841 ccggtttatc tacatggctg tccagcacta catagagacg ctgcagcgcc ggatcgagga
     3901 ggagcagaaa agcaaaagaa aaggacatga atataccaat attaagtatt ccttggtgga
     3961 ccagacaagt ggtgatcaga gtcccctgcc accctgcacc ccaacgccac cctgtgcaga
     4021 aatgagggag gacagcgccc gagtctatga gaacgtgggc ctcatgcagc agcagaggag
     4081 tttcagagga tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     4141 caaggatgac gatgacaagt gagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc
     4201 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     4261 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     4321 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     4381 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     4441 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     4501 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     4561 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     4621 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     4681 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg
     4741 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg
     4801 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca
     4861 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg
     4921 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg
     4981 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc
     5041 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg
     5101 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg
     5161 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca
     5221 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca
     5281 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg
     5341 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga
     5401 cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg
     5461 ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg
     5521 tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5581 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5641 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5701 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5761 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5821 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5881 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5941 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6001 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6061 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6121 cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6541 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc
     6721 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6781 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     6841 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7021 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7081 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7201 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7261 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7321 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7381 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     7441 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7501 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7561 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7621 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7681 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7741 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7801 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7861 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7921 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7981 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8041 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8101 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8161 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8221 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8281 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8341 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8401 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8461 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8521 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8581 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8641 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8701 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8761 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8821 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8881 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8941 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9001 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9061 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9121 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9181 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9241 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9301 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9361 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9421 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9481 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9541 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9601 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     9721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     9781 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9841 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     9901 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     9961 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10021 taaaacgacg gccagtgcca agctg
//
