LOCUS       V025494                10292 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025494
VERSION     V025494
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4335
                     /label="Prkcb(NM_008855)"
                     /note="Prkcb(NM_008855)"
                     /gene="Prkcb"
     CDS             4342..4407
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4451..4662
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4675..4943
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4972..4981
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5002..5664
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5734..5787
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5788..6387
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6388..6975
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7049..7282
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7354..7475
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7494..7629
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7667..7683)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7691..7707
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7715..7745)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7760..7781
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8069..8657)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8828..9688)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9689..9793)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10267..10283
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgacccggc tgcggggccg
     2341 ccgccgagcg agggcgagga gagcacagtg cgcttcgccc gcaaaggcgc cctccggcag
     2401 aagaacgtgc acgaggtgaa gaaccacaaa ttcaccgccc gcttcttcaa gcagcccacc
     2461 ttctgcagcc actgcaccga cttcatctgg ggcttcggga agcagggatt ccagtgtcaa
     2521 gtctgctgct ttgttgtaca caagcgctgc catgagttcg tcacgttctc ctgccccggt
     2581 gcggacaagg gcccggcctc tgatgacccc cggagcaaac acaagtttaa gatccacacg
     2641 tactccagtc ctaccttctg tgaccactgt ggatcgctgc tgtatggact tattcaccag
     2701 gggatgaaat gcgacacctg tatgatgaac gtgcacaagc gctgcgtgat gaacgtcccc
     2761 agcctctgcg gcactgacca cacggaacgc cgcggccgca tctatatcca ggcccacatc
     2821 gacagagagg ttctcattgt tgttgtaaga gatgctaaaa atctggtacc tatggacccc
     2881 aatggcttgt cagatcccta cgttaaactg aaactgatcc ctgatcccaa aagtgagagc
     2941 aaacagaaga ccaagaccat caagtgttcc ctcaatccag agtggaatga aaccttcaga
     3001 tttcagctga aggaatcaga caaagacaga agactgtctg tagagatctg ggattgggac
     3061 ctgaccagca ggaatgactt catgggatct ctgtcatttg ggatttcaga actacagaaa
     3121 gccggtgtgg atggctggtt caagttacta agccaggaag agggtgaata cttcaatgtg
     3181 cccgtgccgc ccgaaggaag cgagggcaat gaagagctgc ggcagaagtt tgagagagcc
     3241 aagattggcc aaggtaccaa ggctccagaa gaaaagacag ctaacactat ctccaaattt
     3301 gacaacaatg gcaacaggga ccggatgaaa ctgaccgatt ttaacttcct gatggtgctg
     3361 gggaaaggca gctttggcaa ggtcatgctc tcagagcgga agggtacaga tgaactctat
     3421 gccgtgaaga tcctgaagaa ggacgtggtg atccaagatg acgatgtgga gtgcaccatg
     3481 gtggagaagc gggtgctggc cctgcctggg aagcccccgt tcctgactca gctccattcc
     3541 tgcttccaga ccatggaccg cctgtacttt gtgatggagt atgtgaacgg gggtgacctc
     3601 atgtaccaca tccaacaagt tggccgtttc aaggagcccc atgctgtatt ttacgccgca
     3661 gagattgcca tcggtctgtt cttcttgcag agcaagggca ttatttaccg cgacctgaaa
     3721 cttgacaacg tgatgctgga ttctgagggg cacatcaaaa tcgctgactt tggcatgtgt
     3781 aaggagaaca tctgggatgg ggtgacaacc aagacattct gtggcactcc ggactacatt
     3841 gctccagaga tcattgctta tcagccctac gggaagtctg tggactggtg ggcatttgga
     3901 gtcctgctgt atgaaatgtt ggctggccag gcaccgtttg aaggggagga tgaggatgaa
     3961 ctcttccagt caatcatgga acacaatgtg gcgtatccca agtccatgtc caaggaagcc
     4021 gtggcaatct gcaaagggct aatgaccaaa cacccaggca agcgcctggg ttgtgggcct
     4081 gaaggggaac gagacattaa ggagcatgcg tttttccggt atattgactg ggagaaactc
     4141 gaacgcaagg agattcagcc accttataaa ccaaaagctt gtgggcgaaa cgctgaaaac
     4201 ttcgaccggt ttttcacccg ccatccacca gtcctaacac ctcctgacca ggaagtcatc
     4261 aggaatattg accaatcaga attcgaagga ttttcctttg ttaactctga atttttaaaa
     4321 cctgaagtca agagcggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     4381 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     4441 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4501 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4561 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4621 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     4681 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     4741 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     4801 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     4861 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     4921 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     4981 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     5041 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     5101 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     5161 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     5221 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     5281 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     5341 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     5401 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     5461 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     5521 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     5581 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     5641 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     5701 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     5761 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     5821 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5881 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5941 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6001 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6061 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6121 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6181 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6241 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6301 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6361 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     6421 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6481 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6541 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6601 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6661 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6721 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6781 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6841 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6901 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt
     6961 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7021 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     7081 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7141 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7201 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7261 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7321 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7501 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7561 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7621 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     7681 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7741 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7801 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8701 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8761 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8821 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8881 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8941 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9001 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9061 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9121 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9421 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9841 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9901 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9961 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    10021 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    10081 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    10141 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    10201 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    10261 gacgttgtaa aacgacggcc agtgccaagc tg
//
