LOCUS       V029355                11812 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029355
VERSION     V029355
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5838
                     /label="Polr2b(NM_153798)"
                     /note="Polr2b(NM_153798)"
                     /gene="Polr2b"
     CDS             5845..5910
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5954..6165
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6178..6446
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6475..6484
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6505..7167
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7237..7290
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7291..7890
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7891..8479
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8553..8786
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8858..8979
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9019..9154
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9187..9203)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9211..9227
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9235..9265)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9280..9301
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9589..10177)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10348..11208)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11209..11313)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11787..11803
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt atgacgccga cgaggacatg
     2341 caatatgatg aagacgacga tgagatcact ccggatctgt ggcaggaagc atgctggatc
     2401 gtgatcagtt cttactttga tgagaaaggc ttggtcagac aacagctgga ttcctttgat
     2461 gagtttattc agatgtctgt tcaaagaatt gtggaagatg caccaccgat tgacctacag
     2521 gccgaagctc agcacgccag tggagaagtt gaagagccgc caagatattt actgaagttt
     2581 gaacaaattt acctctccaa acctactcat tgggaaagag atggtgcccc ttcgccaatg
     2641 atgccaaatg aagccaggtt aagaaatctc acgtattctg ctccgcttta cgttgacata
     2701 acaaagacag tcattaaaga aggtgaagaa cagctgcaga ctcagcacca gaaaacgttc
     2761 atagggaaaa ttccaattat gctgcgctcc acctactgcc tgttaaatgg cttaacagat
     2821 cgtgacctgt gtgagctgaa tgagtgtcct ttggatcccg gtggctattt tattatcaat
     2881 ggatcagaaa aggttctgat agctcaagag aaaatggcaa caaacacagt ctatgtgttt
     2941 gccaaaaagg attccaagta tgcctataca ggagaatgtc gctcatgcct ggagaattct
     3001 tcccgaccca ccagtactat atgggttagc atgctggcca gaggaggaca gggtgcaaag
     3061 aagagtgcta ttggccagcg catcgtggca acgttaccat acatcaagca agaagttcct
     3121 atcatcattg tgttcagagc actaggcttt gtgtctgata gagatatttt agaacacatc
     3181 atttatgact tcgaggatcc agagatgatg gaaatggtta aaccatctct cgatgaagcg
     3241 tttgtcatcc aagagcagaa tgttgcctta aacttcatcg gttcaagagg ggcaaagcct
     3301 ggtgttacta aggagaaaag aattaaatat gcaaaagaag tcttacagaa agaaatgctc
     3361 cctcacgttg gtgtcagtga tttctgtgag accaaaaagg cctatttctt ggggtatatg
     3421 gttcataggt tgcttctagc agccctaggc agaagagaac tagacgacag agatcactat
     3481 ggcaacaaga gattggatct tgctgggcct ctgcttgcat tcttattcag aggcatgttt
     3541 aagaatttgc tgaaagaagt acgaatctat gcgcagaagt ttattgatcg cgggaaggat
     3601 tttaacttag agctggcaat caagacaagg atcatatctg atgggctaaa atactcttta
     3661 gccactggga actggggtga ccagaagaaa gctcatcaag ccagagctgg agtatcccag
     3721 gtattgaacc gcctgacgtt tgcatctact ctttctcacc tgcgtcgtct aaactctcct
     3781 attggcagag atggcaagct agcgaagccc agacagttgc ataatacctt gtggggaatg
     3841 gtgtgtcctg ctgaaactcc agagggccat gctgtagggc ttgtgaagaa tttagccctg
     3901 atggcttata tttcagttgg atctcaaccc tctccaattt tggaattttt agaagaatgg
     3961 agcatggaaa atttagaaga aatttctcct gcagctattg ctgatgcaac caagattttt
     4021 gttaatggct gctgggttgg aatccataaa gaccccgaac aactcatgaa cacgctaagg
     4081 aagttacggc ggcagatgga catcattgta tccgaagttt ctatgatcag agatattcga
     4141 gagcgggaga ttcggatcta tacagatgca ggccgtattt gtagacccct tctgattgtg
     4201 gaaaagcaga agctgctttt gaagaagaga cacattgacc agttgaaaga gagagaatat
     4261 aacaactaca gttggcagga tctggtagcc agtggggtag tagagtatat tgataccctg
     4321 gaagaggaga ccgtgatgct tgcaatgact ccagatgatt tgcaggagaa ggaagtagct
     4381 tattgttcta cctacaccca ctgtgagatt cacccatcaa tgattcttgg agtctgtgca
     4441 tctattattc cttttcctga tcataaccag tcccctagaa acacatacca gtctgctatg
     4501 ggcaagcagg ctatgggagt ttatatcacc aacttccatg tgcggatgga caccttggcc
     4561 catgttctct actatcctca gaaacccctt gtgactacac ggtctatgga atatctacga
     4621 ttcagagagc tgccagcagg aatcaactca attgtggcca ttgcatccta cacgggctat
     4681 aatcaagaag actctgttat catgaatcgt tcagctgtgg atcgaggctt tttcaggtct
     4741 gttttctacc gatcatacaa agaacaagag tctaaaaagg gatttgatca agaagaagtg
     4801 tttgagaagc ctacacgtga gacatgccag ggaatgaggc atgccatcta tgaaaagttg
     4861 gacgatgacg gtttgatagc tccgggggtc cgagtctccg gagatgacgt tattatcggc
     4921 aagacagtca ccttacccga gaacgaggat gagctggaga gcaccaacag gcgctacacc
     4981 aagagggact gcagcacttt cctcagaact agtgagacgg gcatcgtgga ccaggtcatg
     5041 gtcactctca accaggaagg atataaattt tgtaaaataa gggtgcgctc tgttagaatt
     5101 ccgcagattg gagacaaatt tgctagccga catggtcaga agggaacttg tggtattcag
     5161 tatagacaag aggacatgcc gttcacttgc gaaggcataa ctcctgacat catcataaac
     5221 ccccacgcca tcccctcccg gatgacaatt ggtcacttga tcgaatgtct ccaagggaag
     5281 gtctctgcca acaagggtga aattggtgac gctactccat ttaatgatgc tgtcaatgtg
     5341 cagaagattt ctaatctttt gtctgactat ggctaccatc tcagagggaa cgaggtgctc
     5401 tacaatggat tcactggacg aaaaatcaca tcacagattt ttattggccc cacttactac
     5461 cagcgtttga agcatatggt ggatgacaaa attcattctc gtgctagggg acccattcag
     5521 attcttaaca gacagcccat ggagggcaga tctcgtgatg gtggcctgcg cttcggagaa
     5581 atggaacgag actgtcaaat tgcccacggt gcagcacagt ttctaagaga aagactgttt
     5641 gaagcatcag atccgtatca ggttcatgtc tgtaatcttt gtggaataat ggcaattgct
     5701 aacacgagga ctcatacata tgagtgcagg ggatgccgca ataaaaccca gatttccttg
     5761 gtgcgaatgc cgtacgcgtg caagctgttg ttccaggagc tcatgtccat gagcatcgca
     5821 ccacgaatga tgagtgttgg atccgactac aaagaccatg acggtgatta taaagatcat
     5881 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5941 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6001 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6061 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6121 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     6181 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     6241 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     6301 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     6361 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     6421 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     6481 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     6541 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     6601 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     6661 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     6721 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     6781 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     6841 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     6901 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     6961 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     7021 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     7081 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     7141 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     7201 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     7261 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     7321 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7381 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7441 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7501 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7561 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7621 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7681 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7741 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7801 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7861 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     7921 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7981 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8041 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8101 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8161 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8221 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8281 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8341 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8401 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8461 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     8521 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8581 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     8641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8761 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     8821 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     8881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     9001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9121 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     9181 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9361 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9421 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9481 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9541 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9601 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9661 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9721 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9781 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9841 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9901 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9961 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10021 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10081 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10141 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10201 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10261 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10321 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11221 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11281 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11341 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11401 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11461 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11521 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    11581 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    11641 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    11701 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    11761 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
