LOCUS       V029602                12724 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029602
VERSION     V029602
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6750
                     /label="Plb1(NM_001081407)"
                     /note="Plb1(NM_001081407)"
                     /gene="Plb1"
     CDS             6757..6822
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6866..7077
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7090..7358
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7387..7396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7417..8079
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8149..8202
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8203..8802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8803..9391
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9465..9698
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9770..9891
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9931..10066
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10099..10115)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10123..10139
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10147..10177)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10192..10213
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10501..11089)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11260..12120)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12121..12225)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12699..12715
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agctgtaccc aggtgtgtcc
     2341 ccggtgggac tgctgctgct gctactgctg ggacaagggc cctcccaaat ccatggctct
     2401 tctggagaga acacattggc atggcagtcc cagcaagtgt tttggaccct gaagaatttt
     2461 ccattccctt gcaagccaaa gaagttagaa ttgagtgtgc tttctgagtc agttcactct
     2521 ctgagaccct cagacattaa actcgtggca gccatcggca acccagaaat tcctctagcc
     2581 cctgggtctg gcacgatcaa catggagaaa cctcaaagca tcaagaacca accacaggat
     2641 gtatgcatgg gaatcatgac agttctttca gatatcatca gacatttcag tccttctgtc
     2701 ctgatgccca cgtgttctcc tgggaagggc actgcagtcc acactactgc tgaagatttg
     2761 tggattcagg ctaaagagct ggtgaggcgc ctgaaggaca acccgcaact tgactttgag
     2821 aaggactgga aactcatcac tgtgttcttc agtaacacaa gccagtgtca cctgtgtccc
     2881 tctgctcagc agaaaagtca cttgatgagg cacatggaga tgctgtgggg ggtgctggat
     2941 tacctgcatc atgaggtccc cagagcattt gtgaatttgg tggatctctc tgaggttttg
     3001 gccatggatc ttcagcatca agagactggc ttcagccctg caccagaggt ttgcaaatgc
     3061 acagagacaa cgacgctatc caaagctgtc atgcagtggt cctatcagga agcctgggaa
     3121 gatcttctgg cctctagcaa gttcaacaag catgagacct tcgcggtggt tttccagcct
     3181 ttcttcgatg aaattgaacc acccttgaaa aggtcctcgc cccaggatcc caccacactc
     3241 gccctcagga tctggaatag catgatggaa ccagtgggtc aaaaggatgg gctactcaat
     3301 actgcagaga gaaagacaat gaaatgtccc tctgaggaga gtccctatct gttcacctac
     3361 aaaaatagca actaccaggc cagacgactg aaacctataa caaagcttca gatgaaagaa
     3421 ggatcagaat tcacctgtcc tgacaagaac ccctcgaact caatccccac aacagttcac
     3481 agcttgcggc cagctgatat caagatcata ggagcattgg gtgactcgct cacggcaggc
     3541 aatggagctg gggccagccc ttggaatatc ttggatgtct taactgaata ccgaggcctg
     3601 tcctggagtg tgggcgggga tgagaccatc aaaaccgtga ccaccctacc caacattctc
     3661 cgggaattca acccctcctt gaagggcttt tctgttggca ctgggaaaga aagcacttcc
     3721 cgagcatcct tcaaccaggc cgtagcagga gccaaatctg atggcttagc tggccaggcc
     3781 agaaagctgg tggacctgat gaaggcagac aagacaataa actttcagga agactggaag
     3841 ataatcactg tgtttatagg aggcaatgac ctctgtgctt cctgcagtaa ctcgactcgc
     3901 ttttctcccc aaaacttcat agacaacatc aagaacgccc tggacatcct ccatgcagag
     3961 gttccacggg cctttgtgaa catggcaatg gtgatggaga tcaccccctt gagagaacta
     4021 ttcaatgaac ctacagtctc atgcccacgg aacatcctca gccgactgtg tccttgtgtc
     4081 ctgggccttg gtgataactc agaagaactt tccagcctcg ttcaaaggaa cagggattat
     4141 cagaagaaaa ctgaagagct cattaatagc ggacgatatg acacgaggga taatttcact
     4201 gtggttgtcc agcccttatt tgaaaatgtg tccatgccac ggaccccgga gggcgtgccc
     4261 gacaagtctt tctttgcccc tgactgtttc cactttaatg ccaagactca tgcccgctca
     4321 gccattgccc tctggaagaa catgctggaa cctgtgggcc acaagacaag acataataat
     4381 tttgaaatca aggcccctat cgtgtgtccg aaccaggcct cgccatttct gagcactacc
     4441 aagaacagca acctgggtaa tggaacctgg atggtgtgcg aggagagagc cccttctgcc
     4501 tcaccaccaa cctcagtgca taccctgaga cctgcagaca ttcaagttgt ggcagctctg
     4561 ggagactctc tgactgctgg caatggaatc agctcccaag aaggtaacct caccgatgtc
     4621 tccacacagt atcgaggact gtcctatagt gctggtgggg acaagaccct ggagaatgtg
     4681 accactttgc ccaatatcct acggaaattt aatggaaatc tcacaggcta ctcagtagga
     4741 accggtgact ccagttccgc aaacgctttc cttaaccagg ctgtccctgg ggcgaaggct
     4801 gagaacctta caagccaagt ccggactctg gttcagaaga tgaagagtga caacagagtg
     4861 aacttcaacc gagactggaa ggtcatcacg gtgatgatcg gcgccagtga cttgtgtgac
     4921 ttctgcacgg attcgaatca ttactctgca gccaattttt ttgaccatct ccaaaatgcc
     4981 ttggacattc tacataagga ggtacccaga gccctggtca accttgtgga cttcattaac
     5041 cccagtatca ttcgggaagt attcctgaag aacccagaca agtgcccagt gaatcagtcc
     5101 agtgtcctgt gcaactgtgt tctgaccccg aggaaggatt cctatgagct ggcaaggttg
     5161 gaggccttca ccaaatccta ccagagtagc atgctccaac tggttgagtc aggccgctac
     5221 gacactcgag aggacttctc tgtggtactg cagccctttc tcctcaacac caagctccct
     5281 gtcctagaga acgggaaacc agatacatcc ttctttgccc cagactgcat ccacctaaac
     5341 cagaagttcc acactcagct cgcgagagcc ctctgggcca atatgcttga acccctggga
     5401 aagaaaactg atactttgga cccaaaagga cacatatctt tggcctgccc caccaaggat
     5461 cagcccttcc tgagaacctt ccgaaacagt aactacaagt atcctaccaa gccagccatt
     5521 gagaactggg gcagtgactt cctgtgcaca gagaagagcc cttccagcca ggtacccacc
     5581 tcagttcatg agctccgacc agcagacatc aaggtggtgg cagcaatggg tgactttctg
     5641 actacagcca ccggagctcg gccaagtgga tataaaaggc tagccacgcc ctggaggggg
     5701 ctgtcttgga gcattggagg agatggaaag ttggagaccc ataccacact tcccaacatc
     5761 ctgaagaagt tcaacccttc catcactgga ttctctactg gtaccctgga caacaaggca
     5821 ggattaaatg tggcagaaga aggagccaga gctcaggaca tgccggccca ggctaagacc
     5881 ctggtgaaga agatgaaaag cacacctaca atcaacctac aggaagactg gaagctgatt
     5941 acactcctca ttggaaacaa cgacctgtgt ctttactgtg agaatccgga ggacaactca
     6001 actaaagagt atgtcaagta catccagcaa gccttggaca tcctctatga ggagcttccc
     6061 agagttttca tcaatgtggt ggaagtcatg gagctggctg gcctgcacca cgtccaaggt
     6121 gggaaatgtg ccatgccact ggcagttcag aaaaactgca gttgcttaag acactcccaa
     6181 aacctcacag cgatgcagga gctgaagaaa ctaaactgga acctccagag cggcatctcc
     6241 gagctctcct attggcaccg gtacatggag cgtgaggact tcgcagtcac tgtgcagcct
     6301 ttcttccgga atacctttat cccactgaat gagcgtgagg gcctggacct cactttcttc
     6361 tctgaagact gtttctactt ctcagaccgt gggcatgctg agatggccat tgccctctgg
     6421 aataacatgc tggaaccagt gggctggaag acatcctcca ataacttcat atacaacaga
     6481 accaaactca agtgcccctc acctgaaagg cctttcctct acaccctccg gaatagtcag
     6541 cttcttccag acaaggctga agaaccctcc aatgcactct actgggcagt gccagtggca
     6601 gcaataggtg gcctggcagt tggcatcctt ggagtgatgt tgtggagaac tgtgaaaccc
     6661 gtccaacagg aggaggagga ggaggacact cttccaaata caagtgtgac ccaggatgct
     6721 gtatcagaaa agaggctcaa agctgggaac ggatccgact acaaagacca tgacggtgat
     6781 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     6841 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6901 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     6961 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     7021 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     7081 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     7141 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     7201 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     7261 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     7321 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     7381 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     7441 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     7501 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     7561 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     7621 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     7681 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     7741 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     7801 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     7861 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     7921 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     7981 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     8041 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     8101 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     8161 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     8221 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8281 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8341 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8401 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8461 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8521 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8581 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8641 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8701 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8761 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     8821 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8881 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8941 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9001 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9061 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9121 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9181 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9241 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9301 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9361 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     9421 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     9481 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     9541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     9721 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9901 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9961 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    10021 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
    10081 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10921 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12241 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    12301 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    12361 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    12421 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    12481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    12541 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    12601 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    12661 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    12721 gctg
//
