LOCUS       V024054                11579 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024054
VERSION     V024054
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5622
                     /label="Pik3cg(NM_001146200)"
                     /note="Pik3cg(NM_001146200)"
                     /gene="Pik3cg"
     CDS             5629..5694
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5738..5949
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5962..6230
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6259..6268
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6289..6951
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7021..7074
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7075..7674
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7675..8262
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8336..8569
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8641..8762
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8781..8916
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8954..8970)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8978..8994
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9002..9032)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9047..9068
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9356..9944)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10115..10975)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10976..11080)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11554..11570
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agctggagaa ctatgaacaa
     2341 ccggtggttc taagagagga caacctccgc cggcgccgga ggatgaagcc acgcagcgca
     2401 gcaggcagcc tgtcttccat ggagctcatc cccattgagt tcgtactgcc caccagccag
     2461 cgcatcagca agactccaga aacagcgctg ctgcatgtgg ctggccatgg caatgtggaa
     2521 cagatgaaag ctcaggtgtg gctgcgcgca ctggagacca gtgtggctgc ggagttctac
     2581 caccgattgg gcccggacca attcctcctg ctctaccaga agaaaggaca atggtatgag
     2641 atctatgaca ggtaccaagt ggtgcagacc ctagactgcc tgcattactg gaagttgatg
     2701 cacaagagcc ctggccagat ccacgtggta cagcgacacg taccttctga ggagaccttg
     2761 gctttccaga agcagctcac ctccctgatt ggctatgacg tcactgacat cagcaatgtg
     2821 cacgatgatg agctagagtt cactcgccgc cgtctggtta cgccccgcat ggctgaagtg
     2881 gctggccggg atgccaaact ctatgctatg cacccttggg taacgtccaa acctctccca
     2941 gactacctgt caaaaaagat tgccaacaac tgcatcttca tcgtcatcca ccgcggtacc
     3001 accagccaaa ccatcaaggt ctccgcagat gatactcctg gtaccatcct ccagagcttc
     3061 ttcaccaaga tggccaagaa gaagtcccta atgaatatct cagaaagtca aagtgagcag
     3121 gattttgtat tgcgggtttg tggccgcgat gagtacctgg tgggtgaaac acccctcaaa
     3181 aatttccagt gggtgaggca gtgcctcaag aacggagatg aaatacacct ggtgctcgac
     3241 acgcctccag acccagccct tgatgaggtg aggaaggaag aatggccgct ggtggatgac
     3301 tgcactggag tcaccggcta ccacgagcag ctgaccatcc atggcaagga ccacgagagt
     3361 gtgttcacag tgtctttgtg ggactgcgac cgaaagttca gggtcaagat cagaggcatt
     3421 gatatccctg tcctgcctcg gaacaccgac ctcactgtgt ttgtggaagc gaacatccag
     3481 cacgggcaac aagtcctctg ccaaaggaga accagcccta agcccttcgc agaagaggta
     3541 ctctggaatg tgtggctgga gtttggcatc aaaatcaaag acttgcccaa aggggctcta
     3601 ttgaacctac agatctactg ctgcaaaacc ccatcactgt ccagcaaggc ttctgcagag
     3661 actccaggct ccgagtccaa gggcaaagcc cagcttctct attacgtgaa cttgctgtta
     3721 atagaccacc gtttcctcct ccgccacggg gactatgtgc tccacatgtg gcagatatct
     3781 ggcaaggcag aggagcaggg cagcttcaat gctgacaagc tcacatccgc aaccaatcct
     3841 gacaaggaga actcaatgtc catttccatc ctgctggaca attactgtca ccccatagct
     3901 ttgcctaagc accggcccac ccctgaccca gagggagaca gggttcgggc tgaaatgccc
     3961 aatcagcttc gaaagcaatt ggaggcgatc atagccacag atccacttaa ccccctcaca
     4021 gcagaggaca aagaattgct ctggcatttt cgatatgaaa gcctgaagca tccgaaggct
     4081 taccctaagc tattcagctc agtgaaatgg gggcagcaag aaattgttgc caaaacgtac
     4141 cagctgttag ccagaaggga gatctgggat caaagtgctt tggacgttgg cttaaccatg
     4201 cagctcctgg actgcaactt ttcagacgag aatgtccggg ccattgcagt tcagaaactg
     4261 gagagcttag aggacgatga cgttttacat taccttctcc agctggtaca ggctgtgaaa
     4321 tttgaaccgt accacgacag tgcgctggcc agattcctgc tgaagcgtgg cttgaggaac
     4381 aaaagaatcg gtcacttctt gttctggttc ctgcgaagtg agatcgcaca gtccagacac
     4441 tatcagcaga ggttcgctgt gatcctggag gcgtacctgc gaggctgtgg cacagccatg
     4501 ttgcaggact tcacacagca ggtccatgtg attgagatgt tacagaaagt caccattgat
     4561 attaaatcgc tctcggcaga gaagtatgac gtcagttccc aagttatttc acagcttaag
     4621 caaaagcttg aaagccttca gaactccaat ctccccgaga gctttagagt tccctatgat
     4681 cctggactaa aagccggtac cctggtgatc gagaaatgca aagtgatggc ctccaagaag
     4741 aagcccctgt ggcttgagtt taagtgtgct gatcccacag tcctatccaa cgaaaccatt
     4801 ggaatcatct ttaaacatgg tgatgatctg cgccaagaca tgttgatctt gcagattcta
     4861 cgcatcatgg agtccatttg ggagactgaa tctctggacc tgtgccttct gccttacggt
     4921 tgcatctcaa ctggtgacaa aataggaatg atcgagattg taaaggatgc cacaacgatc
     4981 gctcaaattc agcaaagcac agtgggtaac acgggggcat tcaaagatga agtcctgaat
     5041 cactggctca aggaaaaatg tcctattgaa gaaaagtttc aggccgcagt ggaaaggttt
     5101 gtttactcct gtgcaggcta ctgtgtggcc acatttgttc ttgggatcgg tgacaggcac
     5161 aacgacaaca ttatgatctc agagacagga aacctatttc atatagactt cggacacatt
     5221 cttgggaatt acaagagttt cctgggcatc aataaagaga gagtgccctt cgtcctaacc
     5281 ccagacttct tgtttgtgat gggatcttct ggaaaaaaga caagtccaca cttccagaaa
     5341 ttccaggatg tctgtgttag agcttaccta gctcttcgcc atcacacaaa cctgttgatc
     5401 atcttgttct ccatgatgct gatgacagga atgccccagc tgacaagcaa agaggacatt
     5461 gaatatatcc gggatgccct caccgtggga aaaagcgagg aggacgctaa gaaatatttc
     5521 cttgatcaga tcgaagtctg cagagacaaa ggatggactg tgcagtttaa ctggttccta
     5581 catcttgttc ttggcatcaa acaaggagaa aagcactccg ctggatccga ctacaaagac
     5641 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     5701 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5761 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5941 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     6001 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     6061 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6121 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     6181 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     6241 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     6301 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     6361 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6421 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6481 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6541 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     6601 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     6661 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6721 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     6781 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6841 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6901 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6961 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     7021 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     7081 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7141 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7201 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7261 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7321 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7381 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7441 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7501 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7561 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7621 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     7681 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7741 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7801 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7861 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7921 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7981 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8041 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     8101 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8161 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     8221 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
     8281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8341 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     8401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8581 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8641 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8701 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8761 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     8821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8881 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     8941 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9001 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9061 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9121 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9781 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    10141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    10201 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10261 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    11101 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    11161 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    11221 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    11281 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    11341 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    11401 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    11461 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    11521 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
