LOCUS       V034269                10639 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034269
VERSION     V034269
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5493
                     /label="Pik3cb(NM_029094)"
                     /note="Pik3cb(NM_029094)"
                     /gene="Pik3cb"
     CDS             5500..5565
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5609..5820
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5833..6101
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6128..6727
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6734..7322
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7396..7629
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7701..7822
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7841..7976
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8014..8030)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8038..8054
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8062..8092)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8107..8128
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8416..9004)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9175..10035)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10036..10140)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10614..10630
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcctcct gctatggcag acaaccttga catctgggca
     2341 gtggactcac agattgcatc cgatggcgcc atatccgtcg atttccttct gcccaccggg
     2401 atttatatcc agttggaagt acctcgggaa gctaccattt cttatattaa acagatgtta
     2461 tggaagcaag ttcacaacta cccgatgttt aacctcctca tggacattga ctcgtatatg
     2521 tttgcatgtg tgaatcaaac tgctgtatat gaggaactgg aagacgaaac acgaagactt
     2581 tgtgatgtca gaccttttct tccagttctc aaactagtga ctagaagctg tgaccccgca
     2641 gaaaaattgg actcaaaaat tggggttctt ataggaaaag gtcttcatga gtttgatgcc
     2701 ttgaaggatc ccgaagtgaa tgaatttaga agaaaaatgc gcaaattcag tgaggccaag
     2761 attcagtctc tggtagggtt gtcttggatc gactggctaa agcacacgta tccgcctgag
     2821 cacgagccgt ccgtcctgga gaacttggaa gataaacttt atggaggaaa gctggttgtg
     2881 gctgtgcact ttgaaaatag ccaggatgta tttagttttc aagtgtctcc caatttgaat
     2941 cctataaaaa taaatgaatt ggcaatccag aaacgcctca ctattcgtgg aaaggaagat
     3001 gaagctagcc cctgtgacta tgtgttacag gtcagtggga gagtggagta tgtgtttggc
     3061 gatcatccac taattcagtt ccagtacatc cggaattgtg tgatgaatag aaccctgccc
     3121 cacttcatcc ttgtggaatg ttgtaagatc aagaaaatgt atgaacaaga aatgattgcc
     3181 atagaggctg ccatcaaccg aaactcatcc aaccttcctc tccctttacc accaaagaaa
     3241 acgcgagtta tttctcatat ctgggacaac aacaaccctt tccaaattac cttggttaaa
     3301 ggaaataagc ttaatacaga agaaactgtg aaagttcatg tccgagctgg gctttttcac
     3361 ggaaccgagc tcctgtgtaa aaccgtcgta agctcagaga tatcaggaaa gaacgaccat
     3421 atttggaatg aacaactgga atttgatatt aatatttgtg acttaccaag aatggctcga
     3481 ttatgttttg ctgtttatgc agttttggat aaagtaaaaa cgaagaaatc aacaaagact
     3541 attaatccct ctaagtatca gaccatcagg aaagccggga aagtgcatta tcctgtcgca
     3601 tgggtaaata ccatggtttt tgacttcaaa ggacagctga ggtctggaga cgtcatattg
     3661 catagctggt cttcgtttcc tgatgagctg gaagaaatgc tgaatcccat ggggactgtg
     3721 cagacgaacc catatgctga gaacgccacc gccttgcaca ttacgttccc agagaataag
     3781 aagcagccgt gttattatcc ccccttcgat aagatcattg agaaggcagc tgagcttgcc
     3841 agcggagaca gtgctaatgt gtcaagtcgt ggtggaaaaa aatttcttgc tgtgctgaaa
     3901 gaaatcttgg acagggaccc cctgtctcag ctgtgtgaga acgaaatgga ccttatttgg
     3961 actctacggc aagactgccg agaaaatttc cctcagtcac tgccaaaact actcttgtca
     4021 atcaagtgga ataaacttga agatgttgct cagcttcagg cgctcctgca gatatggccc
     4081 aaactgcccc ccagggaagc cctggaactc ctggatttca actatccaga ccagtatgtc
     4141 cgggaatacg ctgtaggctg ccttcgacag atgagtgatg aagaactctc tcagtatctt
     4201 ttacaattgg tgcaagtttt gaaatatgag ccttttctcg attgtgccct ctccagattc
     4261 ctattagaaa gagcacttga taatcggagg attgggcagt ttctgttttg gcatcttagg
     4321 tcagaggtgc acactcctgc tgtgtccgta cagtttggtg tcatcctgga agcatactgt
     4381 cgaggaagcg tggggcacat gaaagtgctt tccaaacagg tggaagcact caataagtta
     4441 aaaactttaa atagcttaat caaactgaat gcggtgaagc tgagcagagc taagggaaag
     4501 gaggccatgc acacgtgcct gaaacagagt gcttaccggg aggcgctctc tgacctgcag
     4561 tcgccgctga acccctgcgt catcctctca gagctctatg ttgaaaagtg caaatacatg
     4621 gactccaaga tgaagcccct gtggctggtc tacagcagca gagcctttgg agaggactcg
     4681 gttggagtga tctttaaaaa tggtgacgat ttgcggcagg acatgctgac gctgcagatg
     4741 ttgcgcctga tggatctgct ttggaaagaa gctggcttgg acctgcggat gctcccctat
     4801 ggctgcttag caacaggaga tcgctctggc ctcattgagg ttgtgagcac ctctgagaca
     4861 atcgctgaca ttcagctgaa cagtagtaac gtggctgcca cggcagcctt caacaaagac
     4921 gcactcctga actggctcaa ggagtacaac tctggggatg acctggaccg agcgattgag
     4981 gagtttacct tgtcctgtgc tggctactgt gtagcctctt atgtcctcgg cattggtgac
     5041 aggcacagtg acaacatcat ggtgaagaaa accggccagc tcttccacat agattttggg
     5101 catattcttg gaaatttcaa atctaaattt ggcattaaaa gggagcgagt accttttatt
     5161 cttacttatg acttcattca tgtcattcaa caaggaaaaa cgggaaacac tgaaaaattt
     5221 ggcagattcc gccagtgctg tgaagatgcg tatctgattt tacggcggca tgggaatctc
     5281 ttcatcaccc tgtttgccct gatgttgact gcagggctgc ctgagctcac atcggtcaaa
     5341 gatatacagt atcttaagga ctcgcttgcc ttagggaaga gcgaggagga agcactgaag
     5401 cagttcaagc agaagtttga cgaggccctc agggaaagct ggactactaa agtgaactgg
     5461 atggctcaca cagtacggaa agactacagg tccggatccg actacaaaga ccatgacggt
     5521 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5581 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5641 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5701 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5761 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5821 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5881 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5941 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6001 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6061 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6121 acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6181 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6241 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6301 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6361 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6421 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6481 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6541 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6601 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6661 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6721 tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7141 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7321 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7381 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     7441 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7501 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7561 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7621 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7681 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7741 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7801 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7921 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     7981 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8821 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10141 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10201 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10381 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10441 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10501 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10561 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    10621 gacggccagt gccaagctg
//
