LOCUS       V029340                15085 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029340
VERSION     V029340
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..9939
                     /label="Piezo1(NM_001357349)"
                     /note="Piezo1(NM_001357349)"
                     /gene="Piezo1"
     CDS             9946..10011
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        10055..10266
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10279..10547
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             10574..11173
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11180..11768
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11842..12075
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12147..12268
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12287..12422
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12460..12476)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12484..12500
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12508..12538)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12553..12574
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12862..13450)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13621..14481)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14482..14586)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15060..15076
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagccg cacgtgctgg gcgccgggct ctactggctg
     2341 ttgctgccct gcacgctcct ggcggcctcc ctgttacgct tcaatgctct ctcgctggtc
     2401 tatttgctgt ttctactgct gctgccctgg cttccaggcc cctcaagaca cagcatacca
     2461 ggtcacacag gtcgcctgct ccgtgcacta ctctgcctca gcctcctctt cctggtggcc
     2521 caccttgcct ttcagatatg cctacacacc gtgcctcacc tggaccagtt tctgggacaa
     2581 aacggtagcc tttgggtgaa ggtgtctcaa cacatagggg ttacgaggct ggacctgaag
     2641 gacatcttta acaccaccag gctggtagca cctgacctgg gagtgctgct ggcgtcctcc
     2701 ctttgccttg gcctctgtgg acgcctcacg aggaaagccc ggcagagtcg gcgcacccag
     2761 gagctggatg atgacgatga cgatgatgac gacgatgaag acatagatgc tgccccagcc
     2821 gtggggctga agggagcccc tgccctggca accaaacgca ggctgtggct ggcctcccgc
     2881 ttccgggtca cggcccactg gctgctgatg acctctggac ggacgctggt cattgtgttg
     2941 ctggccctgg caggcatagc ccacccttcg gccttctcca gcgtctacct ggtggtgttc
     3001 ctggccatct gcacctggtg gtcctgtcac tttcctctca gccccctggg ctttaacacc
     3061 ctctgtgtca tggtgagctg ttttggtgcc ggccatctca tttgcctata ctgctatcag
     3121 acaccattta tccaggacat gttaccccct gggaacatct gggccaggct atttggtctc
     3181 aagaacttcg tagacctccc taactactcc agccccaacg ccctggtact caacactaag
     3241 cacgcctggc ccatctatgt gagtcccgga atcctgctgc tgctatatta cacagccacc
     3301 tctctcctga agctccacaa gagctgtccc tcagagctga ggaaggagac acccagggag
     3361 gatgaggagc atgagctgga actggaccac ctggagccag agccccaggc tagggacgcc
     3421 acccagggtg agatgcccat gaccacggaa cctgaccttg acaactgcac cgtgcatgta
     3481 ctaaccagcc agagccctgt ccgccagcgt ccagttcgcc ccaggctggc tgagctgaaa
     3541 gagatgtcac cgctacatgg cctgggccac ctcatcatgg accagagcta tgtgtgtgcc
     3601 ctgattgcca tgatggtgtg gagcatcatg taccacagct ggctgacctt cgtcctgctg
     3661 ctctgggcct gcctcatctg gacggtgcgt agccgccacc agctggctat gctctgctcg
     3721 ccctgcatcc tgctgtatgg gctgacgctc tgctgcctga ggtacgtgtg ggccatggaa
     3781 cttcctgagc tgcccaccac cctgggccct gtcagcctgc accagttggg actggaacac
     3841 acacgctacc cttgcctgga cctcggtgcc atgctcctct atctgctcac attctggctc
     3901 cttctgcgtc agttcgtgaa ggagaagctg ctgaagaagc aaaaggtgcc tgcggcgctt
     3961 ttggaggtca cggtggccga cactgagccc acacagaccc agacgctgct gcggagcctg
     4021 ggggagctgg tcaccggcat ctacgtcaaa tactggatct atgtgtgcgc cggcatgttc
     4081 attgtggtca gcttcgccgg ccgcctggtg gtctacaaaa tcgtctacat gttcctcttc
     4141 ctgctgtgcc tcaccttgtt ccaggtctac tacaccctgt ggaggaagct gctgcgtgtc
     4201 ttctggtggc tcgtggtggc ctatacaatg ctcgtgctca tcgctgtgta caccttccag
     4261 ttccaggact tccccaccta ttggcgcaac ctcacgggct tcacggacga gcagttgggc
     4321 gacctgggcc tggagcagtt cagtgtgtcg gagctctttt ccagtatcct catccctggc
     4381 ttcttcctgc tggcctgcat cctgcagctg cactacttcc acagaccgtt catgcagctc
     4441 actgacctgg agcacgtgcc gccaccaggc acccgccacc ctcgatgggc tcacaggcag
     4501 gatgcagtga gcgaggcccc tctgcttgag catcaggagg aagaggaagt cttcagggaa
     4561 gatgggcaga gcatggatgg gccccaccag gccacacagg tccctgaggg tacggccagc
     4621 aagtggggcc tggtggctga ccggctgctg gacctagcgg ccagcttctc ggctgtcctc
     4681 acccgaatcc aggtgttcgt tcggcgcttg ctagaacttc acgtcttcaa gctggtggcc
     4741 ctctacactg tctgggtggc cctgaaagaa gtgtctgtga tgaacctgct gctggtggtg
     4801 ctatgggcct tcgccttgcc ctatccgcgc ttccggccca tggcttcctg cctgtccacc
     4861 gtgtggacct gtatcatcat tgtatgcaag atgctctatc agctcaagat tgtcaacccg
     4921 catgagtact ccagcaactg cactgagccc ttccccaaca ataccaactt gcagcctttg
     4981 gagatcaacc agtctttgct gtaccgtggc cctgttgacc ctgccaactg gtttggggtg
     5041 cggaagggtt accccaactt gggctatatc cagaaccacc tgcagatcct tctgttgctg
     5101 gtgtttgagg ccgtggtgta ccggcgccaa gagcactacc gccggcagca ccagcaggcc
     5161 cctctgcccg cccaggctgt gtgcgcagat ggcacccgcc agaggttgga ccaggaccta
     5221 cttagctgcc tcaagtattt catcaacttc ttcttctaca aattcgggct ggagatctgc
     5281 ttcttgatgg ccgtgaatgt gattgggcag cgtatgaact tcatggtgat cttacacggt
     5341 tgctggttgg tggccatcct tacacgccgg cgccgtgagg ccatcgcccg cctctggcct
     5401 aactactgtc tgttcctcac gctgttcctg ctgtaccagt acctgctgtg tttgggcatg
     5461 ccccctgcac tgtgcattga ctatccatgg cgctggagca aggccatccc catgaattcc
     5521 gccctcatca agtggctgta cctacctgac ttcttcagag cccccaactc caccaacctt
     5581 atcagtgact tcctcctgct gctttgcgcc tcccagcagt ggcaggtctt ctcagcggag
     5641 cgaacggagg agtggcaacg catggcgggc atcaacactg accacctgga gcccctgcgt
     5701 ggggagccca accctatacc caacttcatc cactgcaggt cctatctgga tatgctgaag
     5761 gtggccgtct tccgctacct gttctggctg gtgctcgttg tggtgtttgt tgcgggggcc
     5821 acccgcataa gcatcttcgg gctggggtac ctgctagcct gcttctacct gctgctgttt
     5881 ggcactaccc tgctgcagaa ggacacgcga gcccagctcg tgctgtggga ctgcctcatc
     5941 ctctataatg tcactgtcat catctctaag aatatgctgt cgctcctgtc ctgtgtcttc
     6001 gtggagcaaa tgcagagcaa cttctgctgg gtcatccagc tcttcagcct cgtgtgcaca
     6061 gtcaaaggct actatgatcc caaagagatg atgaccaggg accgggactg cctgctgcct
     6121 gtggaggagg ccgggatcat ctgggacagt atctgcttct tcttcctgct cttgcaacgg
     6181 cgcatctttc tcagccacta cttcctgcat gtcagcgctg acctgaaagc cacagccctg
     6241 caggcatcca ggggctttgc cctctacaat gcagccaacc tgaagagcat caacttccat
     6301 cgccagattg aggagaagtc cctggcccag ctgaaaagac agatgaagcg catccgtgcc
     6361 aaacaggaga agtacaggca gagccaggca agtcgtggcc aactccagtc caaagaccct
     6421 caggatccca gccaggagcc agggcctgac agcccagggg gctcctcccc gccacggaga
     6481 cagtggtggc gcccctggct ggaccacgcc acagtcatcc actctggcga ctacttcctg
     6541 tttgagtcag atagcgagga ggaagaggag gccctacctg aggaccccag gcctgcagct
     6601 cagagtgcct tccagatggc ataccaggca tgggtaacca atgcccagac agtgctgagg
     6661 cagcgtcggg agcgggcacg gcaggagcgg gcagagcagc tggcttctgg aggtgacttg
     6721 aacccagatg tggaaccagt agatgtccca gaagatgaga tggcaggccg tagccacatg
     6781 atgcagcgtg tgctaagcac catgcagttc ctgtgggtgc tgggccaggc cacggtagac
     6841 gggctgacgc gctggctgcg tgcattcacg aagcaccacc gcaccatgag cgatgtgctg
     6901 tgcgcagagc gctacctgct cacccaggag cttcttcggg ttggagaggt acgccgaggt
     6961 gtgctggacc agctttatgt gggtgaagat gaggccacat tgtcaggtcc catggagacc
     7021 cgggatggac ccagcacagc ctcaagtggg ctgggagccg aagagccttt gagtagcatg
     7081 acagacgaca ccagcagccc cctgagcaca ggctataaca cccgcagtgg cagtgaggag
     7141 attgtcaccg acgctgggga cctccaggct gggacctccc tgcacggctc ccaagagctt
     7201 ttagccaatg ctcgtacccg gatgcgcacg gccagcgagc tgctactgga taggcgcctg
     7261 catatccctg agctggagga ggccgagcgg tttgaggcac agcagggccg gactctgcgg
     7321 ctgctcaggg ctgggtacca gtgcgtggcg gcacactcgg agctgctctg ttacttcatc
     7381 atcatcctta accacatggt gacagcctcg gctgcctccc tggtgctgcc cgtgcttgtg
     7441 ttcctgtggg ccatgctgac catcccgagg cctagcaagc gcttttggat gacagctatc
     7501 gtcttcactg aggtcatggt ggtcaccaaa tacctgttcc agttcggctt cttcccctgg
     7561 aacagctacg ttgtgctgcg gcgctatgag aacaagccct acttccctcc gcgaatcctg
     7621 ggccttgaga aaacggacag ctacatcaag tatgacctgg tgcagctcat ggccctcttc
     7681 ttccaccgct cgcagctact gtgttatggc ctctgggacc atgaggagga tcgctatccc
     7741 aaggaccatt gcaggagtag tgtgaaggac cgggaggcca aggaagagcc agaagctaag
     7801 ctggaatcgc agtctgagac aggcactggg catcccaagg agccagtgtt ggccggtact
     7861 cccagggacc acatccaagg gaaaggaagt attagatcca aggatgttat ccaagatccc
     7921 ccagaggacc ttaagccccg gcacacgagg cacatcagca tacgcttcag gaggcgcaag
     7981 gagactccag gacccaaagg aacagcagtc atggagactg agcacgagga gggagaagga
     8041 aaagaaacta cagagagaaa gaggccgcgt cacactcaag aaaaatcgaa gtttcgggag
     8101 agaatgaagg cagctgggcg ccggctgcag agcttctgtg tgtcactggc ccagagcttc
     8161 taccaaccct tgcagcgttt cttccatgac attctgcaca caaagtaccg ggcggccacc
     8221 gacgtctacg ccctcatgtt cctggccgat attgtcgaca tcatcatcat catctttggt
     8281 ttttgggctt ttgggaagca ctctgcagcc acagacattg catcctcgct gtcagatgac
     8341 caggtgccac aggccttcct gttcatgctg ctggtccagt ttggcaccat ggtcatcgac
     8401 cgtgccctct acctgcgcaa gactgtcctg ggaaagctgg cctttcaggt ggtcctggtg
     8461 gtggcgattc acatctggat gttctttatc ttaccggctg tcactgagag gatgttcagc
     8521 cagaatgcgg tggcacagct gtggtacttc gtcaagtgca tttactttgc cctgtccgcc
     8581 taccagatcc gctgtggcta ccccacccgt atcttgggca acttcctcac caagaaatac
     8641 aaccatctaa acctcttcct tttccagggg ttccgtctag tgccgttcct ggtggagctg
     8701 cgggccgtca tggactgggt gtggaccgac accacgctgt ccctgtccaa ctggatgtgt
     8761 gtggaagaca tctatgccaa catctttatc atcaagtgca gccgagagac agagaagaaa
     8821 tacccccagc ccaaggggca gaagaagaag aaaattgtca agtatggtat gggaggcctc
     8881 attatcctct tcctcatcgc catcatctgg ttccctctgc tcttcatgtc actgatccgc
     8941 tctgtggtcg gggtcgtcaa ccagcccatt gatgtcaccg tcaccctcaa gctaggcggc
     9001 tatgagccac tgttcaccat gagcgcccag cagccatcca ttgtgccatt cacaccccag
     9061 gcctacgagg agctgtccca gcagtttgac ccctatccac tagccatgca gttcattagc
     9121 cagtacagtc ctgaggacat cgtcactgca cagatcgagg gcagctcggg ggcgctgtgg
     9181 cgcatcagcc cacccagccg agcccagatg aagcaggagc tgtacaacgg cacagccgac
     9241 attacactgc gctttacctg gaatttccaa agggacctgg ccaagggtgg cactgtggag
     9301 tatactaatg agaagcacac cttggagctg gcccccaaca gtacggcacg aaggcagctg
     9361 gcccaactgc tcgagggcag acctgaccag tcagtggtca ttccccatct cttccccaag
     9421 tacattcgtg ctcccaatgg gcctgaagcc aaccctgtga agcagctgca gccagatgag
     9481 gaagaggact accttggtgt gcgcatccag ctgcggaggg agcaagtggg cacaggggcc
     9541 tctggggagc aagcgggcac caaggcctcc gacttcctcg agtggtgggt catcgagctg
     9601 caggactgca aggctgactg caacctgctg cccatggtca tcttcagtga caaggtcagc
     9661 ccacctagcc tgggcttcct ggccggctac gggattgtgg ggctgtacgt ctccatcgtg
     9721 ctggtggttg gcaagtttgt gcggggcttc ttcagcgaga tctctcactc catcatgttc
     9781 gaggaactgc cgtgtgtgga ccgcatcctc aagctgtgcc aggacatctt cttggtgcgc
     9841 gagacccggg agctggagct ggaggaggag ctatacgcca agctcatctt cctgtaccga
     9901 tctccagaga ccatgattaa gtggacacgt gagagggagg gatccgacta caaagaccat
     9961 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
    10021 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
    10081 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
    10141 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
    10201 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
    10261 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
    10321 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
    10381 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
    10441 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
    10501 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
    10561 gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
    10621 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
    10681 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
    10741 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
    10801 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
    10861 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
    10921 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    10981 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    11041 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    11101 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    11161 gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
    11221 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    11281 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    11341 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    11401 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    11461 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    11521 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    11581 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    11641 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    11701 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    11761 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
    11821 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
    11881 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    11941 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    12001 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    12061 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    12121 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    12181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12241 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    12301 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    12361 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    12421 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
    12481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    12541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    12601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    12661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    12721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    12781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    12841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    12901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    12961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    13021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    13081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    13141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    13201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    13261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    13321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    13381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    13441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    13501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    13561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    13621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    13681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    13741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    13801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    13861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    13921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    13981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    14041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    14101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    14161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    14221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    14281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    14341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    14401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    14461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    14521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    14581 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    14641 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    14701 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    14761 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    14821 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    14881 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    14941 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    15001 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    15061 taaaacgacg gccagtgcca agctg
//
