LOCUS       V025041                12932 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025041
VERSION     V025041
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6975
                     /label="Pcf11(NM_029078)"
                     /note="Pcf11(NM_029078)"
                     /gene="Pcf11"
     CDS             6982..7047
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7091..7302
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7315..7583
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7612..7621
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7642..8304
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8374..8427
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8428..9027
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9028..9615
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9689..9922
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9994..10115
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10134..10269
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10307..10323)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10331..10347
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10355..10385)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10400..10421
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10709..11297)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11468..12328)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12329..12433)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12907..12923
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cagagcagac gccggcggag
     2341 gccggggctg cgggggcccg ggaggacgcc tgtcgggatt atcagtcgtc gctggaagac
     2401 ctgaccttca atagcaaacc gcacatcaat atgctgacca ttctagccga ggagaacctg
     2461 cccttcgcca aggagatcgt ctctctcatc gaggcccaaa ccgccaaggc tccttcctca
     2521 gagaagcttc cagttatgta ccttatggat tctatcgtga aaaatgttgg aagagaatat
     2581 ctcactgcct ttactaaaaa tctagttgca acatttattt gtgtgtttga aaaggtggat
     2641 gaaaatacta gaaaaagttt atttaaatta cgttccacat gggatgaaat attccctttg
     2701 aagaaacttt atgccttgga tgtcagagtc aattcattag atcctgcttg gcctattaaa
     2761 cctctgcctc ctaatgtgaa tacatctagc atccatgtga atcccaaatt tttaaataaa
     2821 tcgcctgatg agccttcaac acctggcaca gtggtcagtt cccccagtat ctccactcct
     2881 cccattgtac ctgatataca aaagaatctt acccaagaac aactaataag gcaacagtta
     2941 ctggcaaaac aaaaacagtt gttagaactt cagcagaaaa agctggagct tgagttagaa
     3001 caagctaagg cacagctggc agtatctctg agtgttcagc aggaaacagc caacttgggt
     3061 cctggatcag tgccgtctaa attacatgtt ccacaaattc ccacaatggc tgttaaaact
     3121 ccccatcagg tccctgtaca acctgacaaa agccgagcag gtccatcctt acaaatgcaa
     3181 gacttgaaag gaactaaccg ggatccccgt cttaacagga tgagccaaca ttcttcccat
     3241 ggcaaagagc agagtcatag gaaagaattt gtaatgaaca caataaacca gtctgatatt
     3301 aaaacaagta agaatgtacc ctctgaaaag ctaaattcat ccaaacaaga aaaaagtaaa
     3361 tcaggtgaaa gaataaccaa gaaagaactt gaccagttag attctaaatc caaatctaag
     3421 tctaaatcac catcaccttt gaaaaacaaa ttatcccaca caaaagactt gaaaaatcaa
     3481 gactctgaaa gtatgaggtt gtctgatatg agtaagagag atccacgatt aaagaagcat
     3541 cttcaggata aagctgaggg caaagatgaa gatgtaaaag aaaagagaaa aactgcagaa
     3601 aagaaggaga aagatgagca catgaaatca tctgaacaca gggtgattgg aagtagaagt
     3661 aaaatcataa atggcattgt ccaaaaacaa gacatggtta cagaggagtt ggaaaaacag
     3721 gggacaaaac cagggagatc gagtactaga aagagatcaa gatcaaggtc acctaagtct
     3781 cggtcaccaa ttatacattc cccaaagaga agagatagac ggtcacccaa acgaaggcaa
     3841 agaagtatgt ctcccaattt ggcacccaaa gctggaaaga tgcgtcagtc gggactaaaa
     3901 cagtcacata tggaagaatt cccaccacct tccagggaag aaagaaatat taagagaagt
     3961 gctaagcagg atgttagaga tcccagacga ctgaaaaaga tggatgagga ccggcctcaa
     4021 gaaactgcag gtcaacattc tatgaagtca ggtggtgacc caaaggagaa tatagagaat
     4081 tggcaaagct ctaagtctgc caaaagatgg aaatctggct gggaagaaaa taaaagcttg
     4141 cagcagggtg atgaacatag taaacctcct catctaaggc atagggagag ctggtcaagc
     4201 actaaaggga tcttgtcacc tcgagccccc aagcagcagc accgactaag tgtagatgcc
     4261 aatcttcaaa ttcctaaaga gttaactctt gcaagcaaaa gagaattgct tcaaaagacg
     4321 agcgaacgtt tagcatctgg tgaaattaca caggatgagt tccttgttgt tgtgcatcaa
     4381 attcgacagc tatttcagta tcaagaaggt gtacgagagg agcagagatc accattcaat
     4441 gatcgttttc cacttaagcg acctagatat gaagattcag ataaaccatt tgtagatggc
     4501 ccagcatcaa gatttgctgg ccttgataca aatcagcgac ttacagctct agctgaagac
     4561 cgaccattat ttgatggacc tggtaggcca tctgtgacga gagatggccc agccaagatg
     4621 atttttgaag gacctaataa attaagccct agaattgatg gacctcctac accaggttct
     4681 cttcggtttg atgggtcacc aggacaaatg gggggaggag gccctatgag atttgaagga
     4741 ccacaaggtc agttaggagg tgggtgtcct ttgagatttg aaggtcctcc aggaccagta
     4801 ggaacacctc tgcggtttga agggccaatt ggtcaaggag gaggaggtgg ttttcgattt
     4861 gaaggttcac ctagtttgag gtttgaggga tctacaggtg gtttacgatt tgaaggacca
     4921 gggggtcagc ccgtgggtgg tctcaggttt gaaggacatc gtggtcaacc tgtgggtggt
     4981 ctcaggtttg aaggacctca tggacagcct gtgggcagtc ttagatttga taatcctcga
     5041 ggtcagcctg taggaggact tagatttgaa gggggtcatg gtccatcagg ggctgcaatt
     5101 aggtttgatg ggcctcatgg tcagccaggt ggtggtggtg gtatcagatt tgagggccct
     5161 ttgctacagc aaggagttgg aatgaggttt gagggtcccc atggtcagtc tgtagctggt
     5221 ctgagatttg aaggacataa tcaactaggt gggaacctta ggtttgaggg accacatggt
     5281 caaccagggg ttgggatcag gtttgaagga cctatagttc aacaaggagg tggaatgagg
     5341 tttgaaggtc ctgtaccagg aggtggcctg agaattgaag ggcctctggg tcaaggtggt
     5401 cctagatttg aaggttgtca ctctttaagg tttgatggac agccaggtca accatcactt
     5461 ttgccaagat ttgatggatt acatggccag ccaggtccta gatttgaaag aactggtcag
     5521 ccaggtccac agaggtttga tggaccacct ggacagcagg ttcaaccaag atttgatggt
     5581 gtacctcaaa gatttgatgg gccacaacac cagcaagcat caagatttga tattcctctt
     5641 ggtctacaag gaactcgatt tgacaatcat ccttcacaaa ggattgaatc tttcaatcat
     5701 tctggcccat ataatgatcc acctggcaat acttttaatg ttccatctca aggattacag
     5761 ttccaaagac acgaacaaat atttgataca cctcaaggac caaattttaa tggaccacat
     5821 ggccctggaa atcagaattt cccaaatccc attaacagag cttctggaca ctattttgat
     5881 gaaaagaatc ttcagagttc tcagtttgga aactttggca atttgcctac accaatatca
     5941 gtaggaaata ttcaggcatc tcaacaggtt cttactggtg ttgctcagcc agtagcgttt
     6001 ggccaaggac aacagttctt accagttcat ccacagaatc ctggagcttt tattcaaaat
     6061 ccttcaggcg gtcttcccaa ggcatatcct gataatcatc tcagtcaggt ggatgtaaac
     6121 gaattgtttt caaaactgct aaaaacagga attctcaaat tgtcacagcc tgattcagct
     6181 acagcacagg taaccgaggc tgttgctcag cctccccctg aggaggacga ggatcagaat
     6241 gaagatcagg atgttccaga tcttaccaat ttcaccattg aagaattgaa gcaacgttat
     6301 gacagtgtta taaaccggct gtacactggg attcaatgtt actcgtgtgg aatgaggttc
     6361 acgacatccc agacagatgt ttatgcagat cacttggatt ggcattatcg gcaaaacaga
     6421 accgagaaag atgttagcag aaaagtcact catagacgtt ggtactacag tctaacagat
     6481 tggatagaat ttgaagaaat agctgatctg gaagaacgtg caaaaagcca gttttttgaa
     6541 aaggttcatg aagaggttgt ccttaaaact caggaggctg ctaaagaaaa ggagttccaa
     6601 agtgtacctg ctggaccagc tggagcagtt gagagttgtg aaatctgtca agaacaattt
     6661 gaacagtact gggatgaaga ggaggaagaa tggcacttaa aaaatgctat tagagtagac
     6721 ggaaagattt accatccatc atgttatgaa gattatcaaa atacatcttc atttgattgc
     6781 acaccatctc ccagcaagac accagttgaa aaccctttga acattatgtt gaacattgtc
     6841 aaaaacgaat tgcaagaacc ctgtgaaagt cccaaagtta aggaagaaca aattgatgcc
     6901 ccaccagctt gttcagaaga aagtgtagca acacccactg aaattaaaac agaaagtgat
     6961 acagttgagt cagttggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     7021 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     7081 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     7141 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     7201 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     7261 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     7321 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     7381 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     7441 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     7501 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     7561 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     7621 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     7681 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     7741 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     7801 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     7861 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     7921 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     7981 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     8041 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     8101 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     8161 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     8221 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     8281 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     8341 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     8401 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     8461 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8521 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8581 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8641 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8701 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8761 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8821 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8881 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     8941 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     9001 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     9061 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     9121 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9181 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9241 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9301 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9361 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9421 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9481 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9541 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt
     9601 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9661 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     9721 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9781 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9841 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9901 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     9961 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
    10021 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10081 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
    10141 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    10201 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10261 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
    10321 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    10381 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    10441 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10501 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10561 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10621 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    11041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    11101 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    11161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    11221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    11281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    11341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    11401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    11461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    11641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    12061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    12121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    12181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    12241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    12301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    12361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    12421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    12481 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    12541 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    12601 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    12661 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    12721 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    12781 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    12841 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    12901 gacgttgtaa aacgacggcc agtgccaagc tg
//
