LOCUS       V034587                10656 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034587
VERSION     V034587
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4851
                     /label="PRMT9(NM_138364)"
                     /note="PRMT9(NM_138364)"
                     /gene="PRMT9"
     CDS             4858..4884
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4888..4914
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4918..4944
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5009..5220
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5233..5501
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5530..5539
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5560..6222
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6292..6345
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6346..6744
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6751..7339
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7413..7646
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7718..7839
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7858..7993
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8031..8047)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8055..8071
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8079..8109)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8124..8145
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8433..9021)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9192..10052)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10053..10157)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10631..10647
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgaactcgcg gcccaggtcc
     2341 cgccgagacg ccgggggtgg cgctggggca gccggccggg acgagctggt gtcgcggtcc
     2401 ttgcagagcg cagagcactg tctgggcgtc caggacttcg gcactgccta tgcccactac
     2461 ctcctcgtgc tcagcctggc gccggagctg aaacacgacg tgaaggaaac ttttcagtac
     2521 acacttttca gatgggctga agagcttgat gctctcagtc ggatacaaga cttacttggt
     2581 tgctatgagc aggccttgga actgtttcct gatgatgaag tgatttgcaa tagtatgggg
     2641 gagcatctct tcagaatggg ctttagggat gaagcagctg ggtattttca taaagcagtg
     2701 aagctaaacc ctgatttcag tgatgcaaag gagaattttt atcgtgttgc aaactggttg
     2761 gtggaacgct ggcactttat catgcttaat gacaccaaga ggaatacaat ttataatgca
     2821 gcaatccaaa aggcagtttg tttggggtcc aaaagtgttt tggacattgg agcaggaact
     2881 ggaatactaa gcatgtttgc taaaaaagct ggagcacatt ccgtgtatgc ctgtgagtta
     2941 tccaagacca tgtatgaact tgcctgtgat gtcgtggcag caaacaagat ggaagcaggg
     3001 atcaaactct tacatacgaa gtcacttgac atagagattc caaaacatat tcccgaaaga
     3061 gtgtccctag ttgtaacaga aactgtcgat gcaggtttat ttggagaagg aattgtggag
     3121 agtttgattc atgcatggga gcatttactt ttacagccaa agaccaaagg tgaaagtgct
     3181 aattgtgaaa agtatgggaa agttatacca gcaagtgctg ttatatttgg gatggcagta
     3241 gaatgtgcag agataagaag acatcataga gtgggtatta aggacattgc tggtatccat
     3301 ttgccaacaa atgtgaaatt tcagagtccg gcttattctt ctgtagatac tgaagaaaca
     3361 attgaacctt atacaactga aaagatgagt cgagttcctg gaggatattt ggctttgaca
     3421 gagtgctttg aaattatgac agtagatttc aacaaccttc aggaattaaa aagtcttgca
     3481 actaaaaagc ctgataagat tggtattcct gttattaaag aaggcatact agatgctatt
     3541 atggtttggt ttgtgctcca gcttgatgat gaacatagtt tatccacaag tcctagtgag
     3601 gaaacatgtt gggaacaggc tgtctacccc gtacaggacc ttgcagacta ctggataaag
     3661 cctggagacc atgtgatgat ggaagtatct tgtcaagact gttacttaag aatccagagt
     3721 attagtgtct tgggtttgga atgtgaaatg gatgttgcaa aaagttttac ccagaataaa
     3781 gacttgttat cgttaggaaa tgaggctgaa ctttgtagtg ccctcgctaa ccttcagacc
     3841 agtaaaccag atgctgtaga gcagacatgt atattggaat ctacagaaat tgctttgctt
     3901 aacaacatcc catatcatga aggctttaaa atggcaatga gcaaagtttt gtcttcactg
     3961 actccagaga aactgtatca gaccatggat actcactgtc agaatgagat gagctctgga
     4021 actggacaga gtaatactgt acagaacatc cttgaacctt tctacgtgtt agatgtgtcc
     4081 gaaggcttct ctgttctgcc tgttattgct ggcacacttg ggcaggttaa accatacagt
     4141 tctgtggaga aagaccagca tcgtattgct ctggacctca tatctgaagc caatcacttt
     4201 cctaaagaaa cacttgagtt ttggctgaga catgtggagg atgaatctgc tatgttacaa
     4261 aggccaaaat cagacaagtt atggagcata attatattgg atgtcattga gccatctggg
     4321 ctcattcagc aggaaataat ggaaaaagct gcaatatcca ggtgtttact acaatctgga
     4381 ggcaagatct ttcctcagta tgtgctgatg tttgggttgc ttgtggaatc acagacactc
     4441 ctagaggaga atgctgttca aggaacagaa cgtactcttg gattaaatat agcacctttt
     4501 attaaccagt ttcaggtacc tatacgtgta tttttggacc tatcctcatt gccctgtata
     4561 cctttaagca agccagtgga actcttaaga ctagatttaa tgactccgta tttgaacacc
     4621 tctaacagag aagtaaaggt atacgtttgt aaatctggaa gactgactgc tattccattt
     4681 tggtatcata tgtaccttga tgaagagatt aggttggata cttcaagtga agcctcccac
     4741 tggaaacaag ctgcagttgt tttagataat cccatccagg ttgaaatggg agaggaactt
     4801 gtactcagca ttcagcatca caaaagcaat gtcagcatca cagtaaagca actcgagtac
     4861 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat
     4921 ccttatgatg tcccagacta cgcataggga tccctgcgat atccagcgcg gccgcgaagg
     4981 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5041 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5101 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5161 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5221 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5281 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5341 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5401 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5461 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5521 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5581 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5641 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5701 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5761 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5821 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5881 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5941 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6001 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6061 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6121 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6181 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6241 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6301 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatggc caagcctttg
     6361 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     6421 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     6481 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     6541 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     6601 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     6661 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     6721 tctggttatg tgtgggaggg ctaagtcgac aatcaacctc tggattacaa aatttgtgaa
     6781 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6841 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6901 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6961 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7021 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7081 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7141 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7201 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7261 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7321 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     7381 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa
     7441 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7501 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7561 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7621 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7681 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7981 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg
     8041 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8101 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8161 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8221 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    10201 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
    10261 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    10321 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10381 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    10441 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
    10501 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10561 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10621 tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
