LOCUS V042355 9358 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042355 VERSION V042355 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4212 /label="PRMT5(NM_006109)" /note="PRMT5(NM_006109)" /gene="PRMT5" CDS 4219..4284 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4328..4539 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4552..4820 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4847..5446 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5453..6041 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6115..6348 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6420..6541 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6560..6695 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6733..6749) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6757..6773 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6781..6811) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6826..6847 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7135..7723) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7894..8754) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8755..8859) /gene="bla" /label="AmpR promoter" primer_bind 9333..9349 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcggcg atggcggtcg ggggtgctgg tgggagccgc 2341 gtgtccagcg ggagggacct gaattgcgtc cccgaaatag ctgacacact aggggctgtg 2401 gccaagcagg ggtttgattt cctctgcatg cctgtcttcc atccgcgttt caagagggag 2461 ttcattcagg aacctgctaa gaatcggccc ggtccccaga cacgatcaga cctactgctg 2521 tcaggaaggg actggaatac gctaattgtg ggaaagcttt ctccatggat tcgtccagac 2581 tcaaaagtgg agaagattcg caggaactcc gaggcggcca tgttacagga gctgaatttt 2641 ggtgcatatt tgggtcttcc agctttcctg ctgcccctta atcaggaaga taacaccaac 2701 ctggccagag ttttgaccaa ccacatccac actggccatc actcttccat gttctggatg 2761 cgggtaccct tggtggcacc agaggacctg agagatgata taattgagaa tgcaccaact 2821 acacacacag aggagtacag tggggaggag aaaacgtgga tgtggtggca caacttccgg 2881 actttgtgtg actatagtaa gaggattgca gtggctcttg aaattggggc tgacctccca 2941 tctaatcatg tcattgatcg ctggcttggg gagcccatca aagcagccat tctccccact 3001 agcattttcc tgaccaataa gaagggattt cctgttcttt ctaagatgca ccagaggctc 3061 atcttccggc tcctcaagtt ggaggtgcag ttcatcatca caggcaccaa ccaccactca 3121 gagaaggagt tctgctccta cctccaatac ctggaatact taagccagaa ccgtcctcca 3181 cctaatgcct atgaactctt tgccaagggc tatgaagact atctgcagtc cccgcttcag 3241 ccactgatgg acaatctgga atctcagaca tatgaagtgt ttgaaaagga ccccatcaaa 3301 tactctcagt accagcaggc catctataaa tgtctgctag accgagtacc agaagaggag 3361 aaggatacca atgtccaggt actgatggtg ctgggagcag gacggggacc cctggtgaac 3421 gcttccctgc gggcagccaa gcaggccgac cggcggataa agctgtatgc tgtggagaaa 3481 aacccaaatg ccgtggtgac gctagagaac tggcagtttg aagaatgggg aagccaagtg 3541 accgtagtct catcagacat gagggaatgg gtggctccag agaaagcaga catcattgtc 3601 agtgagcttc tgggctcatt tgctgacaat gaattgtcgc ctgagtgcct ggatggagcc 3661 cagcacttcc taaaagatga tggtgtgagc atccccgggg agtacacttc ctttctggct 3721 cccatctctt cctccaagct gtacaatgag gtccgagcct gtagggagaa ggaccgtgac 3781 cctgaggccc agtttgagat gccttatgtg gtacggctgc acaacttcca ccagctctct 3841 gcaccccagc cctgtttcac cttcagccat cccaacagag atcctatgat tgacaacaac 3901 cgctattgca ccttggaatt tcctgtggag gtgaacacag tactacatgg ctttgccggc 3961 tactttgaga ctgtgcttta tcaggacatc actctgagta tccgtccaga gactcactct 4021 cctgggatgt tctcatggtt tcccatcctc ttccctatta agcagcccat aacggtacgt 4081 gaaggccaaa ccatctgtgt gcgtttctgg cgatgcagca attccaagaa ggtgtggtat 4141 gagtgggctg tgacagcacc agtctgttct gctattcata accccacagg ccgctcatat 4201 accattggcc tcggatccga ctacaaagac catgacggtg attataaaga tcatgacatc 4261 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc 4321 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 4381 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 4441 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 4501 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 4561 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 4621 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4681 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 4741 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 4801 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa 4861 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 4921 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 4981 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 5041 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 5101 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 5161 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 5221 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 5281 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 5341 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 5401 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc 5461 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5521 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5581 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5641 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5701 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5821 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 5881 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5941 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 6001 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa 6061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 6121 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 6181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 6241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 6301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 6361 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 6421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6541 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 6601 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 6661 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 6721 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6781 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6841 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6901 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6961 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7021 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7081 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7141 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7201 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7261 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7321 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7381 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7441 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7501 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7561 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7621 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7681 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7741 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7801 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7861 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7921 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7981 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8041 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8101 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8161 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8221 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8281 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8341 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8401 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8461 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8521 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8581 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8641 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8701 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8761 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8821 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8881 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 8941 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9001 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9061 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9121 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9181 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9241 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9301 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //