LOCUS       V047047                11374 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047047
VERSION     V047047
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2279..2288
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2288..2359
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2366..3322
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID
                     engineered for improved catalytic efficiency (Branon et
                     al., 2018)"
                     /transl_table=1
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="TurboID"
     CDS             3353..5482
                     /label="PREP(NM_002726)"
                     /note="PREP(NM_002726)"
                     /gene="PREP"
     promoter        5533..5744
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5757..6025
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6054..6063
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6084..6746
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6816..6869
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6870..7469
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7470..8057
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8131..8364
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8436..8557
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8576..8711
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8749..8765)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8773..8789
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8797..8827)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8842..8863
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9151..9739)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9910..10770)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10771..10875)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11349..11365
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggac tacaaggatg acgatgacaa ggattacaaa gacgacgatg ataaggacta
     2341 taaggatgat gacgacaaag aattcaaaga caatactgtg cctctgaagc tgatcgctct
     2401 cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag
     2461 ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt
     2521 gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct
     2581 gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta
     2641 cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca
     2701 ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct
     2761 gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg
     2821 aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc
     2881 caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat
     2941 aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt
     3001 ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga
     3061 caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga
     3121 gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag
     3181 acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa
     3241 acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat
     3301 cagtctgcgg tctgccgaaa agggtggagg cggatcagga ggcggtggat ctatgctgtc
     3361 ccttcagtac cccgacgtgt accgcgacga gaccgccgta caggattatc atggtcataa
     3421 aatttgtgac ccttacgcct ggcttgaaga ccccgacagt gaacagacta aggcctttgt
     3481 ggaggcccag aataagatta ctgtgccatt tcttgagcag tgtcccatca gaggtttata
     3541 caaagagaga atgactgaac tatatgatta tcccaagtat agttgccact tcaagaaagg
     3601 aaaacggtat ttttattttt acaatacagg tttgcagaac cagcgagtat tatatgtaca
     3661 ggattcctta gagggtgagg ccagagtgtt cctggacccc aacatactgt ctgacgatgg
     3721 cacagtggca ctccgaggtt atgcgttcag cgaagatggt gaatattttg cctatggtct
     3781 gagtgccagt ggctcagact gggtgacaat caagttcatg aaagttgatg gtgccaaaga
     3841 gcttccagat gtgcttgaaa gagtcaagtt cagctgtatg gcctggaccc atgatgggaa
     3901 gggaatgttc tacaactcat accctcaaca ggatggaaaa agtgatggca cagagacatc
     3961 taccaatctc caccaaaagc tctactacca tgtcttggga accgatcagt cagaagatat
     4021 tttgtgtgct gagtttcctg atgaacctaa atggatgggt ggagctgagt tatctgatga
     4081 tggccgctat gtcttgttat caataaggga aggatgtgat ccagtaaacc gactctggta
     4141 ctgtgaccta cagcaggaat ccagtggcat cgcgggaatc ctgaagtggg taaaactgat
     4201 tgacaacttt gaaggggaat atgactacgt gaccaatgag gggacggtgt tcacattcaa
     4261 gacgaatcgc cagtctccca actatcgcgt gatcaacatt gacttcaggg atcctgaaga
     4321 gtctaagtgg aaagtacttg ttcctgagca tgagaaagat gtcttagaat ggatagcttg
     4381 tgtcaggtcc aacttcttgg tcttatgcta cctccatgac gtcaagaaca ttctgcagct
     4441 ccatgacctg actactggtg ctctccttaa gaccttcccg ctcgatgtcg gcagcattgt
     4501 agggtacagc ggtcagaaga aggacactga aatcttctat cagtttactt cctttttatc
     4561 tccaggtatc atttatcact gtgatcttac caaagaggag ctggagccaa gagttttccg
     4621 agaggtgacc gtaaaaggaa ttgatgcttc tgattaccag acagtccaga ttttctaccc
     4681 tagcaaggat ggtacgaaga ttccaatgtt cattgtgcat aaaaaaggca taaaattgga
     4741 tggctctcat ccagctttct tatatggcta tggcggcttc aacatatcca tcacacccaa
     4801 ctacagtgtt tccaggctta tttttgtgag acacatgggt ggtatcctgg cagtggccaa
     4861 catcagagga ggtggcgaat atggagagac gtggcataaa ggtggtatct tggccaacaa
     4921 acaaaactgc tttgatgact ttcagtgtgc tgctgagtat ctgatcaagg aaggttacac
     4981 atctcccaag aggctgacta ttaatggagg ttcaaatgga ggcctcttag tggctgcttg
     5041 tgcaaatcag agacctgacc tctttggttg tgttattgcc caagttggag taatggacat
     5101 gctgaagttt cataaatata ccatcggcca tgcttggacc actgattatg ggtgctcgga
     5161 cagcaaacaa cactttgaat ggcttgtcaa atactctcca ttgcataatg tgaagttacc
     5221 agaagcagat gacatccagt acccgtccat gctgctcctc actgctgacc atgatgaccg
     5281 cgtggtcccg cttcactccc tgaagttcat tgccaccctt cagtacatcg tgggccgcag
     5341 caggaagcaa agcaaccccc tgcttatcca cgtggacacc aaggcgggcc acggggcggg
     5401 gaagcccaca gccaaagtga tagaggaagt ctcagacatg tttgcgttca tcgcgcggtg
     5461 cctgaacgtc gactggattc cataaggatc cgcggccgcg aaggatctgc gatcgctccg
     5521 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     5581 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     5641 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     5701 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     5761 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     5821 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     5881 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     5941 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     6001 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca
     6061 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc
     6121 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca
     6181 tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc
     6241 acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct
     6301 tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg
     6361 gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact
     6421 tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc
     6481 gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct
     6541 tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca
     6601 tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct
     6661 tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg
     6721 ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg
     6781 acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat
     6841 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg
     6901 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6961 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     7021 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     7081 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     7141 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     7201 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     7261 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     7321 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     7381 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     7441 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa
     7501 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7561 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7621 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7681 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7741 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7801 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7861 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7921 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7981 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
     8041 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8101 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat
     8161 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8341 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8401 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     8461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8641 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8701 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt
     8761 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8821 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8881 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8941 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9001 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9061 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10921 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10981 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    11041 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11101 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    11161 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    11221 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    11281 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    11341 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
