LOCUS       V023570                10790 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023570
VERSION     V023570
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4791
                     /label="PRDM1(NM_001198)"
                     /note="PRDM1(NM_001198)"
                     /gene="PRDM1"
     CDS             4798..4824
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4828..4854
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4858..4884
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4949..5160
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5173..5441
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5470..5479
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5500..6162
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6232..6285
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6286..6885
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6886..7473
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7547..7780
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7852..7973
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7992..8127
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8165..8181)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8189..8205
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8213..8243)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8258..8279
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8567..9155)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9326..10186)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10187..10291)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10765..10781
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tggatatttg cttggaaaaa
     2341 cgtgtgggta cgaccttggc tgcccccaag tgtaactcca gcactgtgag gtttcaggga
     2401 ttggcagagg ggaccaaggg gaccatgaaa atggacatgg aggatgcgga tatgactctg
     2461 tggacagagg ctgagtttga agagaagtgt acatacattg tgaacgacca cccctgggat
     2521 tctggtgctg atggcggtac ttcggttcag gcggaggcat ccttaccaag gaatctgctt
     2581 ttcaagtatg ccaccaacag tgaagaggtt attggagtga tgagtaaaga atacatacca
     2641 aagggcacac gttttggacc cctaataggt gaaatctaca ccaatgacac agttcctaag
     2701 aacgccaaca ggaaatattt ttggaggatc tattccagag gggagcttca ccacttcatt
     2761 gacggcttta atgaagagaa aagcaactgg atgcgctatg tgaatccagc acactctccc
     2821 cgggagcaaa acctggctgc gtgtcagaac gggatgaaca tctacttcta caccattaag
     2881 cccatccctg ccaaccagga acttcttgtg tggtattgtc gggactttgc agaaaggctt
     2941 cactaccctt atcccggaga gctgacaatg atgaatctca cacaaacaca gagcagtcta
     3001 aagcaaccga gcactgagaa aaatgaactc tgcccaaaga atgtcccaaa gagagagtac
     3061 agcgtgaaag aaatcctaaa attggactcc aacccctcca aaggaaagga cctctaccgt
     3121 tctaacattt cacccctcac atcagaaaag gacctcgatg actttagaag acgtgggagc
     3181 cccgaaatgc ccttctaccc tcgggtcgtt taccccatcc gggcccctct gccagaagac
     3241 tttttgaaag cttccctggc ctacgggatc gagagaccca cgtacatcac tcgctccccc
     3301 attccatcct ccaccactcc aagcccctct gcaagaagca gccccgacca aagcctcaag
     3361 agctccagcc ctcacagcag ccctgggaat acggtgtccc ctgtgggccc cggctctcaa
     3421 gagcaccggg actcctacgc ttacttgaac gcgtcctacg gcacggaagg tttgggctcc
     3481 taccctggct acgcacccct gccccacctc ccgccagctt tcatcccctc gtacaacgct
     3541 cactacccca agttcctctt gcccccctac ggcatgaatt gtaatggcct gagcgctgtg
     3601 agcagcatga atggcatcaa caactttggc ctcttcccga ggctgtgccc tgtctacagc
     3661 aatctcctcg gtgggggcag cctgccccac cccatgctca accccacttc tctcccgagc
     3721 tcgctgccct cagatggagc ccggaggttg ctccagccgg agcatcccag ggaggtgctt
     3781 gtcccggcgc cccacagtgc cttctccttt accggggccg ccgccagcat gaaggacaag
     3841 gcctgtagcc ccacaagcgg gtctcccacg gcgggaacag ccgccacggc agaacatgtg
     3901 gtgcagccca aagctacctc agcagcgatg gcagccccca gcagcgacga agccatgaat
     3961 ctcattaaaa acaaaagaaa catgaccggc tacaagaccc ttccctaccc gctgaagaag
     4021 cagaacggca agatcaagta cgaatgcaac gtttgcgcca agactttcgg ccagctctcc
     4081 aatctgaagg tccacctgag agtgcacagt ggagaacggc ctttcaaatg tcagacttgc
     4141 aacaagggct ttactcagct cgcccacctg cagaaacact acctggtaca cacgggagaa
     4201 aagccacatg aatgccaggt ctgccacaag agatttagca gcaccagcaa tctcaagacc
     4261 cacctgcgac tccattctgg agagaaacca taccaatgca aggtgtgccc tgccaagttc
     4321 acccagtttg tgcacctgaa actgcacaag cgtctgcaca cccgggagcg gccccacaag
     4381 tgctcccagt gccacaagaa ctacatccat ctctgtagcc tcaaggttca cctgaaaggg
     4441 aactgcgctg cggccccggc gcctgggctg cccttggaag atctgacccg aatcaatgaa
     4501 gaaatcgaga agtttgacat cagtgacaat gctgaccggc tcgaggacgt ggaggatgac
     4561 atcagtgtga tctctgtagt ggagaaggaa attctggccg tggtcagaaa agagaaagaa
     4621 gaaactggcc tgaaagtgtc tttgcaaaga aacatgggga atggactcct ctcctcaggg
     4681 tgcagccttt atgagtcatc agatctaccc ctcatgaagt tgcctcccag caacccacta
     4741 cctctggtac ctgtaaaggt caaacaagaa acagttgaac caatggatcc tctcgagtac
     4801 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat
     4861 ccttatgatg tcccagacta cgcataggga tccctgcgat atccagcgcg gccgcgaagg
     4921 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4981 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5041 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5101 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5161 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5221 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5281 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5341 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5401 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5461 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5521 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5581 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5641 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5701 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5761 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5821 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5881 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5941 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6001 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6061 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6121 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6181 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6241 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6301 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6361 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6421 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6481 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6541 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6601 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6661 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6721 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6781 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6841 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6901 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6961 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7021 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7081 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7141 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7201 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7261 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7321 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7381 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag
     7441 acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     7501 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7561 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7621 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7681 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7741 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7801 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7981 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8041 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8101 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     8161 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8221 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8281 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8341 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8401 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8461 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9001 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10321 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10381 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    10441 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10501 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10561 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10621 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10681 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10741 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
