LOCUS       V036810                10504 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036810
VERSION     V036810
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5358
                     /label="PLEKHM2(NM_015164)"
                     /note="PLEKHM2(NM_015164)"
                     /gene="PLEKHM2"
     CDS             5365..5430
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5474..5685
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5698..5966
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5993..6592
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6599..7187
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7261..7494
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7566..7687
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7706..7841
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7879..7895)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7903..7919
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7927..7957)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7972..7993
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8281..8869)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9040..9900)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9901..10005)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10479..10495
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagccg ggggaggtga aggaccggat cctggagaac
     2341 atctcgctgt cggtgaagaa gttgcagagc tattttgctg catgtgagga tgagatccct
     2401 gccatccgga accatgacaa ggtcctacag cgtctgtgtg agcacctgga ccacgccctg
     2461 ctgtacggac tgcaagacct ctcctctggc tactgggtgc tcgtggtgca ttttactcgg
     2521 agagaggcca tcaagcagat cgaggtgctg cagcacgtgg ccaccaacct ggggcgcagc
     2581 cgtgcctggc tgtacctggc cctcaacgag aactccttgg agagctacct gcggttgttc
     2641 caggagaacc tgggcctgct gcataagtac tacgtcaaga atgccctggt ctgcagccac
     2701 gatcacctga cgctcttcct gaccttggtg tccgggctag agttcattcg tttcgagctg
     2761 gatctggatg ccccttacct agacctggcc ccctacatgc ccgactacta caaacctcag
     2821 tacctgctgg actttgaaga ccgccttccc agctcggtcc acggctcaga cagtctgtcc
     2881 ctcaactctt tcaactccgt cacctccacc aacctggagt gggatgacag tgcgattgcc
     2941 ccatctagtg aggattatga ttttggagat gtgtttccag cagtgccgtc tgtacccagc
     3001 acagactggg aagatggaga cctcacagac acggtcagtg gtccccgctc cacagcctcc
     3061 gacctgacca gcagcaaggc ctccaccagg agccccaccc agcgccagaa ccccttcaac
     3121 gaggagccgg cagagactgt gtcctcctct gacaccaccc ccgtgcacac cacctctcag
     3181 gagaaggagg aggcccaggc cctggacccg ccggatgcct gcacggagct cgaggtcatc
     3241 agggtcacca agaagaagaa aattggcaag aagaaaaaga gcagatcaga tgaggaggca
     3301 agtccactcc accccgcctg cagccagaag aaatgtgcca agcaggggga cggtgacagc
     3361 cgcaacggca gcccaagcct tgggcgggac tcgccagaca ctatgcttgc ctccccccag
     3421 gaggagggag aggggccgag cagcaccacg gagagcagcg agcgctccga gccgggcctg
     3481 ctgatccctg agatgaagga cacctccatg gagcgcttgg ggcagcccct gagcaaggtt
     3541 atcgaccagc tcaacgggca gctggacccc agcacctggt gctcccgtgc tgagccccca
     3601 gaccagtcct ttcggaccgg ctctcccggg gatgccccgg agaggccgcc gctttgcgac
     3661 tttagtgagg ggctttcagc cccaatggac ttctaccgct ttaccgtcga gagtccaagc
     3721 actgttacat caggtggcgg ccaccatgac cctgcagggc ttggccaacc gctgcatgtt
     3781 cctagtagcc ctgaggctgc tggccaagaa gaagagggag gaggaggaga gggacagacg
     3841 cctcggcccc tagaggatac cacgagggag gctcaggagc tggaggccca gctgtccctg
     3901 gtcagggagg ggcctgtgtc tgagccagag cctgggaccc aggaggttct ctgccagctc
     3961 aagcgagacc agcccagccc gtgtctgagt agcgctgagg attctggggt ggatgaggga
     4021 caggggagcc cttcggagat ggtccattcc tcggagttca gagtagacaa caatcacctg
     4081 ctcctgctca tgatccacgt gttccgagaa aacgaagagc agctgttcaa aatgatccgg
     4141 atgagcaccg ggcacatgga gggcaacctg cagctgctgt acgtgctgct cacagactgc
     4201 tatgtctacc tgctccggaa aggggccaca gagaagccat acctggtgga agaggccgtt
     4261 tcttacaatg aacttgacta tgtgtcggtt ggccttgacc agcagacggt gaagctggtg
     4321 tgcaccaacc gcaggaagca gtttctgctg gacacggctg atgtggcgct ggctgagttc
     4381 tttttggctt ctttgaagtc agccatgatc aaaggctgtc gagaacctcc ctaccccagc
     4441 atcctgacgg atgccaccat ggagaagctg gcactggcca aatttgtggc ccaagaatcg
     4501 aagtgtgagg catctgctgt caccgtgcgc ttctacggcc ttgtgcactg ggaggacccc
     4561 acagacgagt ccctgggccc cacgccctgc cactgctcac cccccgaggg caccatcacc
     4621 aaagaaggca tgctgcacta caaggcgggc acctcctacc tgggcaagga acactggaag
     4681 acgtgcttcg tggtgctcag caacgggatc ctctaccagt acccggaccg caccgacgtc
     4741 atccctctgc tctcggtgaa catggggggg gagcagtgcg gtggctgccg gagagccaac
     4801 accacggatc ggccccacgc cttccaggtc attctctccg accggccctg cctggagcta
     4861 agtgccgaga gcgaggccga gatggccgag tggatgcagc atctctgcca ggctgtgtcc
     4921 aaaggggtca tcccccaggg cgtagctccc agcccctgca taccctgctg cctggtcctc
     4981 acggatgacc gcctctttac gtgccatgag gattgccaga ccagcttctt ccgctctttg
     5041 ggcacagcca agctgggcga catcagcgcc gtctccaccg agccgggcaa ggagtactgc
     5101 gtcttggagt tctcccagga cagccagcag ctcctcccgc cctgggtcat ctacctgagc
     5161 tgcacttctg aactggaccg attgctgtct gcactgaact ctgggtggaa aaccatctat
     5221 caggtggacc tcccccacac ggcgatccag gaagcctcca acaagaagaa attcgaggat
     5281 gccttgagcc tcatccacag cgcctggcag cggagcgaca gtctctgccg cggccgagcc
     5341 tcccgagacc cctggtgcgg atccgactac aaagaccatg acggtgatta taaagatcat
     5401 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5461 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5521 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5581 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5641 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5701 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5761 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5821 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5881 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5941 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     6001 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6061 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6121 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6181 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6241 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6301 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6361 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6421 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6481 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6541 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     6601 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6661 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6721 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6781 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6841 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6901 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6961 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7021 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7081 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7141 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     7201 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7261 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     7321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7501 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7561 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7621 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7681 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     7741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7801 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     7861 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7921 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7981 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8041 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8701 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10021 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10081 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10141 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10201 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10261 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10321 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10381 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10441 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10501 gctg
//
