LOCUS       V027173                12106 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027173
VERSION     V027173
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6132
                     /label="PLEKHA7(NM_001410960)"
                     /note="PLEKHA7(NM_001410960)"
                     /gene="PLEKHA7"
     CDS             6139..6204
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6248..6459
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6472..6740
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6769..6778
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6799..7461
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7531..7584
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7585..8184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8185..8773
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8847..9080
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9152..9273
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9313..9448
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9481..9497)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9505..9521
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9529..9559)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9574..9595
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9883..10471)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10642..11502)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11503..11607)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12081..12097
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcggcgac ggtcgggcgg
     2341 gacactttac ctgagcattg gtcctacggg gtgtgccggg atggccgcgt cttcttcatc
     2401 aatgaccagc tccgctgcac gacctggctg catccgcgca ccggggagcc cgtcaactcg
     2461 ggccacatga tccgctcaga cctgccccgc ggctgggagg agggcttcac ggaggagggc
     2521 gccagctact tcatcgacca taaccagcag accacagcat tcaggcatcc tgtgacggga
     2581 cagttttctc cagaaaatag tgaattcatt cttcaagaag agccgaatcc acatatgtcg
     2641 aagcaagaca gaaaccaaag accgtccagc atggtcagtg aaacatccac ggctgggacc
     2701 gcctccaccc tggaggccaa gcctggaccc aagatcataa agtccagcag taaagtccac
     2761 agctttggga agagagacca ggccattcgg aggaacccca atgttcccgt ggtggtgagg
     2821 ggctggctgc acaagcagga cagttctggg atgaggctgt ggaaaaggag gtggtttgtg
     2881 cttgctgatt actgcttatt ttactataaa gacagccgag aagaagcggt cctcgggagc
     2941 atccccttgc ccagctacgt gatctctcct gtggcccctg aggatcgcat aagccgcaaa
     3001 tattccttta aggctgtgca cacggggatg cgagcgctca tctataacag ctccacagcg
     3061 ggctctcagg ccgagcagtc aggcatgagg acctactact tcagtgccga cacccaggag
     3121 gacatgaacg cttgggtcag ggccatgaac caggctgcac aggtgctgtc tcgatcgtca
     3181 ctgaagaggg atatggagaa ggtggagcgg caggctgtcc cccaggccaa ccacacagag
     3241 tcctgtcacg aatgtggccg ggtgggaccc ggacatacga gagattgtcc tcatcgtggc
     3301 catgatgaca ttgtcaactt cgagaggcag gagcaggagg gagagcagta ccgttcccag
     3361 agggacccac tggagggcaa gcgggaccgg agcaaggcca ggtctccgta ctcgccagcc
     3421 gaggaggatg ccttgtttat ggatttaccc actggcccaa gaggccagca ggcacagccc
     3481 caacgggcag agaagaatgg aatgctgcct gcctcatatg gcccaggaga acagaatggg
     3541 actggtgggt accagcgggc ctttcctccc aggaccaacc ctgaaaaaca cagccaaagg
     3601 aagagcaatc tggcccaggt ggagcactgg gcaagggccc agaaagggga tagcaggagt
     3661 cttcccttgg accagacgct tcctcgccag ggtcctggcc aatccctgtc cttcccagaa
     3721 aactaccaga ctcttcccaa gagcacccga cacccctcgg ggggctcctc gccacctccc
     3781 cgaaacctgc caagtgacta caagtatgcg caggaccgag ccagccacct gaagatgtcg
     3841 agtgaagagc gccgggcgca ccgggatggc accgtgtggc agctctacga gtggcagcag
     3901 cgccagcagt tccggcacgg cagccccaca gcgcccatct gccttggctc cccagagttc
     3961 accgaccagg gccggagcag gagcatgcta gaggtgcccc gctccatctc tgtgcctcca
     4021 tctccctcgg acatccctcc cccaggaccc ccaagggtct tcccaccccg gcggccacac
     4081 acaccagcag agcgagtcac agtgaagcca ccggaccaga ggaggagtgt ggacatctcg
     4141 ctgggggatt ctccaaggag ggcacggggc cacgctgtca agaactcatc tcatgtggac
     4201 cgacgctcca tgccctccat gggttacatg acccacaccg tcagcgctcc cagtttacat
     4261 ggaaaatcgg ctgatgatac ctacctccag ctgaagaaag acctggagta cctggatcta
     4321 aagatgacag gccgggacct tctcaaggat cgaagtctga agcctgtgaa gatcgctgag
     4381 agcgacactg acgtcaaact gagcatcttc tgtgaacaag acagggtcct ccaggacttg
     4441 gaagacaaga tacgagccct taaagagaac aaagaccagc tagaatctgt gctggaggtg
     4501 ttgcacagac agatggagca gtaccgagac cagccccagc acttggagaa gattgcctac
     4561 cagcagaagt tgctgcagga ggaccttgtc catatccgag ctgagctctc cagagagtcc
     4621 actgagatgg aaaatgcttg gaacgaatac ctgaagttgg agaatgatgt ggaacagctg
     4681 aagcagaccc tgcaggagca acacagaaga gccttttttt tccaggagaa atcgcagata
     4741 cagaaagatc tatggagaat tgaagatgtc actgcaggcc tgagtgcaaa taaagagaac
     4801 ttcagaattc tagtggagtc agtaaaaaat ccggagagaa aaacggtgcc tttgtttcct
     4861 cacccgcctg tgccttcact ctcaacttct gagagcaagc cgcccccaca gcccagtcct
     4921 cccaccagcc ctgtgcggac ccctctggag gttcgactct tcccccagct gcaaacctac
     4981 gtgccgtacc gacctcaccc accccagctg aggaaagtga catcccccct tcagtcacca
     5041 actaaggcga agcccaaagt tcaggaagat gaagcacctc ccaggccccc actccccgaa
     5101 ctctacagcc cagaggacca gcccccggct gtgccgcctc tgccaagaga ggccaccatc
     5161 atccggcaca catctgtgcg gggcctcaag cggcagtcag acgagaggaa gcgagaccgg
     5221 gagctggggc agtgtgtgaa tggggattcc agggtggagc tgcggtcgta tgtcagtgag
     5281 cctgagctgg cgaccctcag cggggacatg gcccagccct ccctaggact tgtgggccct
     5341 gagagcaggt accagacgct gccaggcaga gggctctcag ggtccacgtc aaggctccag
     5401 cagtcgtcca ccattgctcc ctacgtcaca ctccggaggg gtctcaatgc cgaaagcagc
     5461 aaggcgacct tccctagacc taagagtgcc ttggagcgcc tgtactcagg ggatcaccag
     5521 cgaggcaaga tgagtgcaga ggagcagctg gagcgcatga agcgacacca gaaggccctg
     5581 gtccgagagc gcaagaggac actgggccaa ggggagagga cgggcctgcc ctcatctcgc
     5641 tacctcagcc ggccgctccc tggagatctt ggctcatgga agcgtgagca ggactttgac
     5701 ctgcagttgc tggaacgggt cgtgcaaggg gaaaaaaagg acaaggagga gaatggctgg
     5761 ttgaaagtgc aggccatgcc tgtcactgag ttggacctgg agcctcaaga ctatgacttg
     5821 gacatcagca gagagctgtc caaaccagag aaggtgtcaa tccctgagcg ctacgtggag
     5881 ctagatcccg aagagccacc cagccttgag gagctgcagg cgcggtaccg caaagccgag
     5941 aagatccgca acatcctcgc ccggtcaagc atgtgcaacc tacagccgac ctcaggccag
     6001 gaccagaaca gtgtggctga cctggacttg cagctgcagg agcaggagcg catcatcaac
     6061 atctcctacg ccctggcctc cgaggcctcc cagcgtagca agcaggtggc agcccaggca
     6121 gtgactgacc cgggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     6181 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     6241 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6301 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6361 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6421 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     6481 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6541 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6601 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6661 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6721 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6781 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6841 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6901 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6961 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     7021 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     7081 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     7141 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     7201 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     7261 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     7321 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7381 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7441 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     7501 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7561 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7621 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7681 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7741 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7801 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7861 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7921 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7981 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8041 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8101 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8161 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     8221 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8281 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8341 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8401 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8461 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8521 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8581 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8641 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8701 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8761 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8821 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8881 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9061 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9121 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     9301 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9361 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9421 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     9481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10321 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    12001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    12061 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
