LOCUS       V034931                10876 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034931
VERSION     V034931
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2290..3294
                     /label="PLAUR(NM_002659)"
                     /note="PLAUR(NM_002659)"
                     /gene="PLAUR"
     CDS             3322..4974
                     /codon_start=1
                     /note="synthetic luc2 version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
                     /label="luciferase"
     promoter        5035..5246
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5259..5527
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5556..5565
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5586..6248
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6318..6371
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6372..6971
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6972..7559
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7633..7866
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7938..8059
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8078..8213
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8251..8267)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8275..8291
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8299..8329)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8344..8365
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8653..9241)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9412..10272)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10273..10377)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10851..10867
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctaga tgggtcaccc gccgctgctg ccgctgctgc tgctgctcca cacctgcgtc
     2341 ccagcctctt ggggcctgcg gtgcatgcag tgtaagacca acggggattg ccgtgtggaa
     2401 gagtgcgccc tgggacagga cctctgcagg accacgatcg tgcgcttgtg ggaagaagga
     2461 gaagagctgg agctggtgga gaaaagctgt acccactcag agaagaccaa caggaccctg
     2521 agctatcgga ctggcttgaa gatcaccagc cttaccgagg ttgtgtgtgg gttagacttg
     2581 tgcaaccagg gcaactctgg ccgggctgtc acctattccc gaagccgtta cctcgaatgc
     2641 atttcctgtg gctcatcaga catgagctgt gagaggggcc ggcaccagag cctgcagtgc
     2701 cgcagccctg aagaacagtg cctggatgtg gtgacccact ggatccagga aggtgaagaa
     2761 gggcgtccaa aggatgaccg ccacctccgt ggctgtggct accttcccgg ctgcccgggc
     2821 tccaatggtt tccacaacaa cgacaccttc cacttcctga aatgctgcaa caccaccaaa
     2881 tgcaacgagg gcccaatcct ggagcttgaa aatctgccgc agaatggccg ccagtgttac
     2941 agctgcaagg ggaacagcac ccatggatgc tcctctgaag agactttcct cattgactgc
     3001 cgaggcccca tgaatcaatg tctggtagcc accggcactc acgaaccgaa aaaccaaagc
     3061 tatatggtaa gaggctgtgc aaccgcctca atgtgccaac atgcccacct gggtgacgcc
     3121 ttcagcatga accacattga tgtctcctgc tgtactaaaa gtggctgtaa ccacccagac
     3181 ctggatgtcc agtaccgcag tggggctgct cctcagcctg gccctgccca tctcagcctc
     3241 accatcaccc tgctaatgac tgccagactg tggggaggca ctctcctctg gacccctgat
     3301 acgaactcgg aattcgccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     3361 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     3421 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     3481 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     3541 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     3601 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     3661 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     3721 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     3781 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     3841 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     3901 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     3961 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     4021 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     4081 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     4141 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     4201 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     4261 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     4321 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     4381 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     4441 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     4501 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     4561 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     4621 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     4681 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     4741 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     4801 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     4861 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     4921 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaggatccc
     4981 tgcgatatcc agcgcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag
     5041 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
     5101 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     5161 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     5221 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
     5281 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     5341 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     5401 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     5461 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     5521 acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg
     5581 gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg
     5641 agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga
     5701 gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct
     5761 tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca
     5821 acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga
     5881 gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg
     5941 gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg
     6001 agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc
     6061 tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca
     6121 tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc
     6181 tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg
     6241 ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg
     6301 gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     6361 atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6421 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6481 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6541 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6601 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6661 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6721 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6781 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6841 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6901 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6961 ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7021 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7081 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7141 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7201 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7261 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7321 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7381 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7441 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7501 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc
     7561 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7621 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     7681 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7741 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7801 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7861 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7921 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8041 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     8101 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8161 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     8221 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8281 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8341 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8401 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9061 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10381 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10441 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10501 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10561 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10621 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10681 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10741 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10801 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10861 ggccagtgcc aagctg
//
