LOCUS       V041532                10468 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041532
VERSION     V041532
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4494
                     /label="PKP1(NM_001005337)"
                     /note="PKP1(NM_001005337)"
                     /gene="PKP1"
     CDS             4501..4566
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4610..4821
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4834..5102
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5131..5140
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5161..5823
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5893..5946
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5947..6546
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6547..7135
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7209..7442
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7514..7635
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7675..7810
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7843..7859)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7867..7883
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7891..7921)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7936..7957
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8245..8833)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9004..9864)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9865..9969)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10443..10459
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga accactcgcc gctcaagacc
     2341 gccttggcgt acgaatgctt ccaggaccag gacaactcca cgttggcttt gccgtcggac
     2401 caaaagatga aaacaggcac gtctggcagg cagcgcgtgc aggagcaggt gatgatgacc
     2461 gtcaagcggc agaagtccaa gtcttcccag tcgtccaccc tgagccactc caatcgaggt
     2521 tccatgtatg atggcttggc tgacaattac aactatggga ccaccagcag gagcagctac
     2581 tactccaagt tccaggcagg gaatggctca tggggatatc cgatctacaa tggaaccctc
     2641 aagcgggagc ctgacaacag gcgcttcagc tcctacagcc agatggagaa ctggagccgg
     2701 cactaccccc ggggcagctg taacaccacc ggcgcaggca gcgacatctg cttcatgcag
     2761 aaaatcaagg cgagccgcag tgagcccgac ctctactgtg acccacgggg caccctgcgc
     2821 aagggcacgc tgggcagcaa gggccagaag accacccaga accgctacag cttttacagc
     2881 acctgcagtg gtcagaaggc cataaagaag tgccctgtgc gcccgccctc ttgtgcctcc
     2941 aagcaggacc ctgtgtatat cccgcccatc tcctgcaaca aggacctgtc ctttggccac
     3001 tctagggcca gctccaagat ctgcagtgag gacatcgagt gcagtgggct gaccatcccc
     3061 aaggctgtgc agtacctgag ctcccaggat gagaagtacc aggccattgg ggcctattac
     3121 atccagcata cctgcttcca ggatgaatct gccaagcaac aggtctatca gctgggaggc
     3181 atctgcaagc tggtggacct cctccgcagc cccaaccaga acgtccagca ggccgcggca
     3241 ggggccctgc gcaacctggt gttcaggagc accaccaaca agctggagac ccggaggcag
     3301 aatgggatcc gcgaggcagt cagcctcctg aggagaaccg ggaacgccga gatccagaag
     3361 cagctgactg ggctgctctg gaacctgtct tccactgacg agctgaagga ggaactcatt
     3421 gccgacgccc tgcctgttct ggccgaccgc gtcatcattc ccttctctgg ctggtgcgat
     3481 ggcaatagca acatgtcccg ggaagtggtg gaccctgagg tcttcttcaa tgccacaggc
     3541 tgcttgagga acctgagctc ggccgatgca ggccgccaga ccatgcgtaa ctactcaggg
     3601 ctcattgatt ccctcatggc ctatgtccag aactgtgtag cggccagccg ctgtgacgac
     3661 aagtctgtgg aaaactgcat gtgtgttctg cacaacctct cctaccgcct ggacgccgag
     3721 gtgcccaccc gctaccgcca gctggagtat aacgcccgca acgcctacac cgagaagtcc
     3781 tccactggct gcttcagcaa caagagcgac aagatgatga acaacaacta tgactgcccc
     3841 ctgcctgagg aagagaccaa ccccaagggc agcggctggt tgtaccattc agatgccatc
     3901 cgcacctacc tgaacctcat gggcaagagc aagaaagatg ctaccctgga ggcctgtgct
     3961 ggtgccctgc agaacctgac agccagcaag gggctgatgt ccagtggcat gagccagttg
     4021 attgggctga aggaaaaggg cctgccacaa attgcccgcc tcctgcaatc tggcaactct
     4081 gatgtggtgc ggtccggagc ctccctcctg agcaacatgt cccgccaccc tctgctgcac
     4141 agagtgatgg ggaaccaggt gttcccggag gtgaccaggc tcctcaccag ccacactggc
     4201 aataccagca actccgaaga catcttgtcc tcggcctgct acactgtgag gaacctgatg
     4261 gcctcgcagc cacaactggc caagcagtac ttctccagca gcatgctcaa caacatcatc
     4321 aacctgtgcc gaagcagtgc ctcacccaag gccgcagaag ctgcccggct tctcctgtct
     4381 gacatgtggt ccagcaagga actgcagggt gtcctcagac agcaaggttt cgataggaac
     4441 atgctgggaa ccttagctgg ggccaacagc ctcaggaact tcacctcccg attcggatcc
     4501 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     4561 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4621 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4681 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4741 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4801 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4861 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4921 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4981 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5041 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5101 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5161 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5221 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5281 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5341 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5401 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5461 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5521 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5581 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5641 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5701 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5761 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5821 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5881 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     5941 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6001 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6061 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6121 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6181 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6241 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6301 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6361 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6421 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6481 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6541 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     7141 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7201 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     7261 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7321 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7381 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7441 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7501 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7561 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7621 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7681 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7741 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7801 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     7861 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7921 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7981 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8041 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8101 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8161 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8221 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8281 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8341 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8401 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8461 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8581 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8641 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8701 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8761 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8821 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8881 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8941 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9001 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9061 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9121 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9181 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9241 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9301 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9361 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9421 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9481 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9541 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9601 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9661 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9721 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9781 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9841 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9901 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9961 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10021 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10081 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10141 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10201 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10261 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10321 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10381 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10441 ttgtaaaacg acggccagtg ccaagctg
//
