LOCUS       V029304                10774 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029304
VERSION     V029304
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4800
                     /label="PI4KB(NM_002651)"
                     /note="PI4KB(NM_002651)"
                     /gene="PI4KB"
     CDS             4807..4872
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4916..5127
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5140..5408
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5437..5446
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5467..6129
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6199..6252
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6253..6852
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6853..7441
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7515..7748
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7820..7941
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7981..8116
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8149..8165)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8173..8189
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8197..8227)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8242..8263
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8551..9139)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9310..10170)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10171..10275)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10749..10765
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gattcttgga agctcgaagt
     2341 ctggctgtgg ccatgggaga tacagtagtg gagcctgccc ccttgaagcc aacttctgag
     2401 cccacttctg gcccaccagg gaataatggg gggtccctgc taagtgtcat cacggagggg
     2461 gtcggggaac tatcagtgat tgaccctgag gtggcccaga aggcctgcca ggaggtgttg
     2521 gagaaagtca agcttttgca tggaggcgtg gcagtctcta gcagaggcac cccactggag
     2581 ttggtcaatg gggatggtgt ggacagtgag atccgttgcc tagatgatcc acctgcccag
     2641 atcagggagg aggaagatga gatgggggcc gctgtggcct caggcacagc caaaggagca
     2701 agaagacggc ggcagaacaa ctcagctaaa cagtcttggc tgctgaggct gtttgagtca
     2761 aaactgtttg acatctccat ggccatttca tacctgtata actccaagga gcctggagta
     2821 caagcctaca ttggcaaccg gctcttctgc tttcgcaacg aggacgtgga cttctatctg
     2881 ccccagttgc ttaacatgta catccacatg gatgaggacg tgggtgatgc cattaagccc
     2941 tacatagtcc accgttgccg ccagagcatt aacttttccc tccagtgtgc cctgttgctt
     3001 ggggcctatt cttcagacat gcacatttcc actcaacgac actcccgtgg gaccaagcta
     3061 cggaagctga tcctctcaga tgagctaaag ccagctcaca ggaagaggga gctgccctcc
     3121 ttgagcccgg cccctgacac agggctgtct ccctccaaaa ggactcacca gcgctctaag
     3181 tcagatgcca ctgccagcat aagtctcagc agcaacctga aacgaacagc cagcaaccct
     3241 aaagtggaga atgaggatga ggagctctcc tccagcaccg agagtattga taattcattc
     3301 agttcccctg ttcgactggc tcctgagaga gaattcatca agtccctgat ggcgatcggc
     3361 aagcggctgg ccacgctccc caccaaagag cagaaaacac agaggctgat ctcagagctc
     3421 tccctgctca accataagct ccctgcccga gtctggctgc ccactgctgg ctttgaccac
     3481 cacgtggtcc gtgtacccca cacacaggct gttgtcctca actccaagga caaggctccc
     3541 tacctgattt atgtggaagt ccttgaatgt gaaaactttg acaccaccag tgtccctgcc
     3601 cggatccccg agaaccgaat tcggagtacg aggtccgtag aaaacttgcc cgaatgtggt
     3661 attacccatg agcagcgagc tggcagcttc agcactgtgc ccaactatga caacgatgat
     3721 gaggcctggt cggtggatga cataggcgag ctgcaagtgg agctccccga agtgcatacc
     3781 aacagctgtg acaacatctc ccagttctct gtggacagca tcaccagcca ggagagcaag
     3841 gagcctgtgt tcattgcagc aggggacatc cgccggcgcc tttcggaaca gctggctcat
     3901 accccgacag ccttcaaacg agacccagaa gatccttctg cagttgctct caaagagccc
     3961 tggcaggaga aagtacggcg gatcagagag ggctccccct acggccatct ccccaattgg
     4021 cggctcctgt cagtcattgt caagtgtggg gatgaccttc ggcaagagct tctggccttt
     4081 caggtgttga agcaactgca gtccatttgg gaacaggagc gagtgcccct ttggatcaag
     4141 ccatacaaga ttcttgtgat ttcggctgat agtggcatga ttgaaccagt ggtcaatgct
     4201 gtgtccatcc atcaggtgaa gaaacagtca cagctctcct tgctcgatta cttcctacag
     4261 gagcacggca gttacaccac tgaggcattc ctcagtgcac agcgcaattt tgtgcaaagt
     4321 tgtgctgggt actgcttggt ctgctacctg ctgcaagtca aggacagaca caatgggaat
     4381 atccttttgg acgcagaagg ccacatcatc cacatcgact ttggcttcat cctctccagc
     4441 tcaccccgaa atctgggctt tgagacgtca gcctttaagc tgaccacaga gtttgtggat
     4501 gtgatgggcg gcctggatgg cgacatgttc aactactata agatgctgat gctgcaaggg
     4561 ctgattgccg ctcggaaaca catggacaag gtggtgcaga tcgtggagat catgcagcaa
     4621 ggttctcagc ttccttgctt ccatggctcc agcaccattc gaaacctcaa agagaggttc
     4681 cacatgagca tgactgagga gcagctgcag ctgctggtgg agcagatggt ggatggcagt
     4741 atgcggtcta tcaccaccaa actctatgac ggcttccagt acctcaccaa cggcatcatg
     4801 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4861 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4921 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4981 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     5041 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     5101 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     5161 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     5221 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5281 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5341 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5401 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5461 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5521 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5581 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5641 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5701 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5761 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5821 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5881 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5941 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     6001 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     6061 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     6121 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     6181 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6241 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6301 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6361 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6421 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6481 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6541 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6601 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6661 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6721 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6781 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6841 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6901 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6961 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7021 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7081 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7141 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7201 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7261 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7321 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7381 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7441 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7501 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7561 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7621 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7681 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7741 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7801 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7921 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8041 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8101 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     8161 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8221 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8281 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8341 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8401 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8461 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8521 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8581 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8641 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8701 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8761 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8821 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8881 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8941 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9001 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9061 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9121 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9181 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9241 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9301 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9361 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9421 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9481 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9541 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9601 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9661 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9721 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9781 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9841 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9901 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9961 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10021 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10081 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10141 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10201 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10261 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10321 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10381 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10441 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10501 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10561 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10621 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10681 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10741 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
