LOCUS       V042606                12910 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042606
VERSION     V042606
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..7764
                     /label="PHIP(NM_017934)"
                     /note="PHIP(NM_017934)"
                     /gene="PHIP"
     CDS             7771..7836
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7880..8091
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8104..8372
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8399..8998
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9005..9593
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9667..9900
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9972..10093
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10112..10247
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10285..10301)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10309..10325
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10333..10363)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10378..10399
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10687..11275)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11446..12306)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12307..12411)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12885..12901
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtcttgt gagaggaaag gcctctcgga gctgcgatcg
     2341 gagctctact tcctcatcgc ccggttcctg gaagatggac cctgtcagca ggcggctcag
     2401 gtgctgatcc gcgaggtggc cgagaaggag ctgctgcccc ggcgcaccga ctggaccggg
     2461 aaggagcatc ccaggaccta ccagaatctg gtgaagtatt acagacactt agcacctgat
     2521 cacttgctgc aaatatgtca tcgactagga cctcttcttg aacaagaaat tcctcaaagt
     2581 gttcctggag tacaaacttt attaggagct ggaagacagt ctttactacg cacaaataaa
     2641 agctgcaagc atgttgtgtg gaaaggatct gctctggctg cgttgcactg tggaagacca
     2701 cctgagtcac cagttaacta tggtagccca cccagcattg cggatactct gttttcaagg
     2761 aagctgaatg ggaaatacag acttgagcga cttgttccaa ctgcagtgta tcagcacatg
     2821 aaaatgcata aacgaattct tggacacttg tcatctgtgt actgtgtaac ttttgatcga
     2881 actggcagac ggatatttac tggttctgat gactgtcttg tgaaaatatg ggcaacagat
     2941 gatgggaggt tgttagctac cttaagagga catgctgctg aaatatcaga catggctgta
     3001 aactatgaga ataccatgat agcagctgga agttgtgata aaatgatccg agtctggtgt
     3061 cttcgaacct gtgcaccttt ggctgttctt cagggccata gtgcatctat tacatcacta
     3121 cagttctcac cattgtgcag tggctcaaag agatatctat cttctactgg ggcagatggc
     3181 actatttgtt tttggctctg ggatgctgga acccttaaaa taaacccaag acctgcaaaa
     3241 tttacagagc gccctcggcc tggagttcaa atgatctgtt cttcttttag tgctggtgga
     3301 atgtttctgg cgacgggaag cacagatcat attattcggg tttatttttt tggatcaggt
     3361 cagccagaga aaatatcaga attggagttt catactgaca aagttgacag tatccagttt
     3421 tccaacacta gtaacaggtt tgtaagtggc agtcgtgatg ggacagcacg tatttggcaa
     3481 tttaaacgaa gagagtggaa gagcattttg ttggatatgg ctactcgtcc agcaggccaa
     3541 aaccttcaag gaatagaaga taaaatcaca aaaatgaagg ttactatggt agcttgggat
     3601 cgacatgaca atacagttat aactgcagtt aataacatga ctctgaaagt ttggaattct
     3661 tacactggtc aactaattca tgtcctgatg ggtcatgaag atgaggtatt tgttcttgaa
     3721 ccacacccgt tcgatcctag agttctcttt tctgctggtc atgatggaaa cgtgatagtg
     3781 tgggatctgg caagaggagt caaaatacga tcttatttca atatgattga aggccaagga
     3841 catggcgcag tatttgactg caaatgctct cctgatggtc agcattttgc atgcacagac
     3901 tctcatggac atcttttaat ttttggcttt gggtccagta gcaaatatga caagatagca
     3961 gatcagatgt tctttcatag tgattatcgg ccacttattc gtgatgccaa caattttgta
     4021 ttagatgaac agactcagca agcacctcat cttatgcctc cccctttttt ggttgatgtt
     4081 gatggtaacc ctcatccatc aagatatcaa agattagttc ctggccgtga aaattgcagg
     4141 gaggagcaac tcatccctca gatgggagta acttcctcag gactgaatca agttttaagt
     4201 cagcaagcaa accaggagat cagcccactg gacagcatga ttcaaagact acaacaggag
     4261 caagacctga gacgttctgg tgaagcagtt atcagtaata ccagccgttt aagtagaggc
     4321 tccataagtt ctacctcaga ggttcattca ccaccaaacg taggactaag acgtagtgga
     4381 caaattgaag gtgtacggca aatgcacagc aacgcaccaa gaagtgaaat agccacagag
     4441 cgggatctgg tagcttggag tcgaagggtg gtagtacccg agctatcagc tggtgtagcc
     4501 agtaggcaag aagaatggag aactgcaaag ggagaagaag aaataaagac ttacaggtca
     4561 gaagagaaaa gaaaacactt aactgttcca aaagagaata aaatacccac tgtctcaaag
     4621 aatcatgctc atgagcattt cctggatctt ggagaatcca aaaagcaaca gacaaatcaa
     4681 cacaattatc gtacaagatc tgcattggaa gagactccta gaccctcaga agagatagaa
     4741 aatggcagta gttcttcaga tgaaggcgaa gtagttgctg tcagtggtgg aacatccgaa
     4801 gaagaagaga gagcatggca cagtgatggc agttctagtg actactccag tgattactct
     4861 gactggacag cagatgcagg aattaatctg cagccaccaa agaaagttcc taagaataaa
     4921 accaagaaag cagaaagcag ttcagatgaa gaagaagaat ctgaaaaaca gaagcaaaaa
     4981 cagattaaaa aggaaaagaa aaaagtaaat gaagaaaaag atggaccaat atcaccaaag
     5041 aaaaagaagc ccaaagaaag aaaacaaaag agattggctg tgggagaact aactgaaaat
     5101 ggtttgacat tagaagaatg gttgccatca acatggatta cagataccat tccccgaaga
     5161 tgtccatttg tgccacagat gggtgatgag gtttattatt tccgacaagg acatgaagcc
     5221 tatgtcgaaa tggcccggaa aaataaaata tatagtatca atcccaaaaa acaaccatgg
     5281 cataaaatgg agctacggga acaagaactt atgaaaatag ttggcataaa gtatgaagtg
     5341 ggattaccta ccctttgctg ccttaaactt gcttttctag atcctgatac tggtaaactg
     5401 actggcggat catttaccat gaaataccat gatatgcctg acgttataga tttcctagtc
     5461 ttgagacaac aatttgatga tgcaaaatac aggcgatgga atataggtga ccgcttcagg
     5521 tctgtcatag atgatgcctg gtggtttgga acaatcgaaa gccaggaacc tcttcaactt
     5581 gagtaccctg atagtctgtt tcaatgctac aatgtttgct gggacaatgg agatacagaa
     5641 aagatgagtc cttgggatat ggagcttata cctaataatg ctgtatttcc tgaagaacta
     5701 ggtaccagtg ttcctttaac tgatggtgag tgcagatcac taatctataa acctcttgat
     5761 ggagaatggg gtaccaatcc cagggatgaa gaatgtgaaa gaattgtggc aggaataaac
     5821 cagttgatga cactagatat tgcctcagca tttgtggccc ccgtggatct gcaagcctat
     5881 cccatgtatt gcacagtagt ggcatatcca acggatctaa gtacaattaa acaaagactg
     5941 gaaaacaggt tttacaggcg ggtttcttcc ctaatgtggg aagttcgata tatagagcat
     6001 aatacacgaa catttaatga gcctggaagc cctattgtga aatctgctaa attcgtgact
     6061 gatcttcttc tacattttat aaaggatcag acttgttata acataattcc actttataat
     6121 tcaatgaaga agaaagtttt gtctgattct gaggatgaag agaaagatgc tgatgtgcca
     6181 ggaacttcta ctcgaaaaag gaaggaccat cagcctagaa gaagattacg taatagagcc
     6241 cagtcttacg atattcaagc atggaagaaa cagtgtgaag aattgttaaa tctcatattt
     6301 caatgtgaag attcagagcc tttccgtcag ccggtagatc tccttgaata tccagactac
     6361 agagacatca ttgacactcc aatggatttt gctaccgtta gagaaacttt agaggctggg
     6421 aattatgagt caccaatgga gttatgtaaa gatgtcagac ttattttcag taattccaaa
     6481 gcatatacac caagcaaaag atcaaggatt tacagcatga gtttgcgcct gtctgctttc
     6541 tttgaagaac acattagttc agttttatca gattataaat ctgctcttcg ttttcataaa
     6601 agaaatacca taaccaaaag gaggaagaaa agaaacagaa gcagctctgt ttccagtagt
     6661 gctgcatcaa gccctgaaag gaaaaaaagg atcttaaaac cccagctaaa atcagaaagc
     6721 tctacctctg cattctctac acctacacga tcaataccgc caagacacaa tgctgctcag
     6781 ataaacggta aaacagaatc tagttctgtg gttcgaacca gaagcaaccg agtggttgta
     6841 gatccagttg tcactgagca accatctact tcttcagctg caaagacttt tattacaaaa
     6901 gctaatgcat ctgcaatacc agggaaaaca atactagaga attctgtgaa acattccaaa
     6961 gctttgaata ctctttccag tcctggtcaa tccagtttta gtcatggcac taggaataat
     7021 tctgcaaaag aaaacatgga aaaggaaaag ccagtcaaac gtaaaatgaa gtcatctgta
     7081 ctcccaaagg cgtccactct ttcaaagtca tcagctgtca ttgagcaagg agattgtaag
     7141 aacaacgctc ttgtaccagg aaccattcaa gtaaatggcc atggaggaca gccatcaaaa
     7201 cttgtgaaga ggggacctgg aaggaaacct aaagtagaag ttaataccaa tagtggtgaa
     7261 attatacaca agaaaagggg tagaaagccc aaaaagctac agtatgcaaa gccagaagat
     7321 ttagagcaaa ataatgtgca tcccatcaga gatgaagtac ttccttcttc aacatgcaat
     7381 tttctttctg aaactaataa tgtaaaggaa gatttgttac agaaaaagaa tcgtggaggt
     7441 aggaagccca aaaggaagat gaagacacaa aaattagatg cagatctcct agtccctgca
     7501 agtgtcaaag tgttaaggag aagtaaccga aaaaagatag atgatcctat agatgaggaa
     7561 gaagagtttg aagaactcaa aggctctgaa ccccacatga gaactagaaa tcaaggtcga
     7621 aggacagctt tctataatga ggatgactct gaagaggagc aaaggcagct gttgttcgaa
     7681 gacacctctt taacttttgg aacttctagt agaggacgag tccgaaagtt gactgaaaaa
     7741 gcaaaagcta atttaattgg ttggggatcc gactacaaag accatgacgg tgattataaa
     7801 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     7861 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     7921 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     7981 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     8041 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     8101 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     8161 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     8221 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     8281 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     8341 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat
     8401 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     8461 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     8521 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8581 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     8641 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8701 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8761 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8821 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8881 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8941 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
     9001 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     9061 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     9121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     9181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     9241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     9301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     9361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     9421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
     9601 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9661 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg
     9721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9901 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9961 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10081 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
    10141 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    10201 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag
    10261 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    10321 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    10381 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    10441 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    10501 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10561 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10621 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11101 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    11161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11341 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    11401 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    11461 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    11521 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    11581 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11641 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11701 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    11761 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    11821 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    11881 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    11941 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12001 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12061 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12121 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12181 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    12241 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    12301 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    12361 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    12421 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    12481 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    12541 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    12601 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    12661 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    12721 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    12781 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    12841 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    12901 tgccaagctg
//
