LOCUS       V021348                10396 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021348
VERSION     V021348
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4510
                     /label="PDE4B(NM_002600)"
                     /note="PDE4B(NM_002600)"
                     /gene="PDE4B"
     promoter        4555..4766
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4779..5047
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5076..5085
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5106..5768
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5838..5891
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5892..6491
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6492..7079
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7153..7386
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7458..7579
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7598..7733
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7771..7787)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7795..7811
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7819..7849)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7864..7885
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8173..8761)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8932..9792)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9793..9897)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10371..10387
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaagaa aagcaggagt gtgatgacgg tgatggctga
     2341 tgataatgtt aaagattatt ttgaatgtag cttgagtaaa tcctacagtt cttccagtaa
     2401 cacacttggg atcgacctct ggagagggag aaggtgttgc tcaggaaact tacagttacc
     2461 accactgtct caaagacaga gtgaaagggc aaggactcct gagggagatg gtatttccag
     2521 gccgaccaca ctgcctttga caacgcttcc aagcattgct attacaactg taagccagga
     2581 gtgctttgat gtggaaaatg gcccttcccc aggtcggagt ccactggatc cccaggccag
     2641 ctcttccgct gggctggtac ttcacgccac ctttcctggg cacagccagc gcagagagtc
     2701 atttctctac agatcagaca gcgactatga cttgtcacca aaggcgatgt cgagaaactc
     2761 ttctcttcca agcgagcaac acggcgatga cttgattgta actccttttg cccaggtcct
     2821 tgccagcttg cgaagtgtga gaaacaactt cactatactg acaaaccttc atggtacatc
     2881 taacaagagg tccccagctg ctagtcagcc tcctgtctcc agagtcaacc cacaagaaga
     2941 atcttatcaa aaattagcaa tggaaacgct ggaggaatta gactggtgtt tagaccagct
     3001 agagaccata cagacctacc ggtctgtcag tgagatggct tctaacaagt tcaaaagaat
     3061 gctgaaccgg gagctgacac acctctcaga gatgagccga tcagggaacc aggtgtctga
     3121 atacatttca aatactttct tagacaagca gaatgatgtg gagatcccat ctcctaccca
     3181 gaaagacagg gagaaaaaga aaaagcagca gctcatgacc cagataagtg gagtgaagaa
     3241 attaatgcat agttcaagcc taaacaatac aagcatctca cgctttggag tcaacactga
     3301 aaatgaagat cacctggcca aggagctgga agacctgaac aaatggggtc ttaacatctt
     3361 taatgtggct ggatattctc acaatagacc cctaacatgc atcatgtatg ctatattcca
     3421 ggaaagagac ctcctaaaga cattcagaat ctcatctgac acatttataa cctacatgat
     3481 gactttagaa gaccattacc attctgacgt ggcatatcac aacagcctgc acgctgctga
     3541 tgtagcccag tcgacccatg ttctcctttc tacaccagca ttagacgctg tcttcacaga
     3601 tttggagatc ctggctgcca tttttgcagc tgccatccat gacgttgatc atcctggagt
     3661 ctccaatcag tttctcatca acacaaattc agaacttgct ttgatgtata atgatgaatc
     3721 tgtgttggaa aatcatcacc ttgctgtggg tttcaaactg ctgcaagaag aacactgtga
     3781 catcttcatg aatctcacca agaagcagcg tcagacactc aggaagatgg ttattgacat
     3841 ggtgttagca actgatatgt ctaaacatat gagcctgctg gcagacctga agacaatggt
     3901 agaaacgaag aaagttacaa gttcaggcgt tcttctccta gacaactata ccgatcgcat
     3961 tcaggtcctt cgcaacatgg tacactgtgc agacctgagc aaccccacca agtccttgga
     4021 attgtatcgg caatggacag accgcatcat ggaggaattt ttccagcagg gagacaaaga
     4081 gcgggagagg ggaatggaaa ttagcccaat gtgtgataaa cacacagctt ctgtggaaaa
     4141 atcccaggtt ggtttcatcg actacattgt ccatccattg tgggagacat gggcagattt
     4201 ggtacagcct gatgctcagg acattctcga taccttagaa gataacagga actggtatca
     4261 gagcatgata cctcaaagtc cctcaccacc actggacgag cagaacaggg actgccaggg
     4321 tctgatggag aagtttcagt ttgaactgac tctcgatgag gaagattctg aaggacctga
     4381 gaaggaggga gagggacaca gctatttcag cagcacaaag acgctttgtg tgattgatcc
     4441 agaaaacaga gattccctgg gagagactga catagacatt gcaacagaag acaagtcccc
     4501 cgtggataca taagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag
     4561 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
     4621 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     4681 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     4741 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
     4801 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     4861 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     4921 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     4981 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     5041 acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg
     5101 gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg
     5161 agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga
     5221 gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct
     5281 tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca
     5341 acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga
     5401 gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg
     5461 gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg
     5521 agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc
     5581 tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca
     5641 tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc
     5701 tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg
     5761 ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg
     5821 gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     5881 atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5941 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6001 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6061 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6121 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6181 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6241 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6301 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6361 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6421 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6481 ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6541 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6601 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6661 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6721 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6781 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6841 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6901 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6961 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7021 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc
     7081 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7141 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     7201 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7381 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7441 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7501 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7561 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     7621 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7681 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     7741 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7801 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7861 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7921 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7981 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8041 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8581 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8881 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8941 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9001 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9061 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9121 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9181 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9241 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9301 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9361 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9421 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9481 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9541 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9601 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9661 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9721 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9781 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9901 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9961 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10021 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10081 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10141 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10201 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10261 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10321 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10381 ggccagtgcc aagctg
//
