LOCUS       V034424                11215 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034424
VERSION     V034424
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5241
                     /label="Nlrx1(NM_001163743)"
                     /note="Nlrx1(NM_001163743)"
                     /gene="Nlrx1"
     CDS             5248..5313
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5357..5568
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5581..5849
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5878..5887
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5908..6570
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6640..6693
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6694..7293
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7294..7882
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7956..8189
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8261..8382
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8422..8557
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8590..8606)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8614..8630
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8638..8668)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8683..8704
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8992..9580)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9751..10611)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10612..10716)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11190..11206
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ggtggggctg ccatttgccc
     2341 aggacctctt ggggctctgg cctgggaaga acaccccagc taccagatga gcatatctcc
     2401 ttcttgatcc agtggagctg gccctttaaa ggggtgcatc ccctgaggcc ccctagggcc
     2461 tttatccgtt accatggaaa ctcggcagac agtgctcccc caccagggag gcatgggcag
     2521 ctgttcagga gcatctctgc cacagaagct atccaaaggc atcgccggaa cctcaccgag
     2581 tggtttagcc gactgcccag agaggagcgc cagtttggac caacctttgc tctagacaca
     2641 gttcatgttg accccgtgat ccgagagagc accccagatg agctgcttcg cccgtccacg
     2701 gagctggcca cggggcatca gcaaacccag gcagggctcc ccccactggc cctgtctcag
     2761 ctttttgacc cggattcttg tgggcgccgc gtgcagaccg tggtgttgta tgggaccgtg
     2821 ggtactggca agagcacgtt ggtacggaag atggtcttag actggtgtta cgggagactg
     2881 cctgcctttg agcttctcat ccccttctcc tgtgaggact tgtcatccct gggctccacc
     2941 ccagcttccc tgtgccaact tgtgacccag cgttacacac ccctgaaaga ggtgttgccc
     3001 ctgatgactg ctgcgggatc ccgcctgctc tttgtgctcc atggcttgga gcgcctcaac
     3061 cttgacttcc ggctggcagg cacagggctt tgcagtgacc cggaggaacc cgggccacca
     3121 gctgccatca tagtcaacct gctgcgcaaa tacatgcttc ccgaggccag cattctggta
     3181 accacccggc cttccaccat tagccgaatc cctagcaagt atgtgggccg ctatggtgag
     3241 atctgtggct tctctgatac caacctgcag aagctctact tccagctccg ccttaaccag
     3301 cctgactgtg ggtacggtgc tgggggtgcc agtgtctcag tcacaccagc tcagcgcgac
     3361 aacctgattc aaatgctctc ccggaacctg gaggggcacc accagattgc cgcagcctgc
     3421 tttctgcctt cctattgctg gcttgtctgt gctactttgc acttcctgca tgctcccaca
     3481 cctgctggtc agaccctcac aagcatctat accagctttc tacgcctgaa cttcagtggg
     3541 gaaacactgg acagcaccca cacgtccaat ctatccctga tgtcctatgc agcccggact
     3601 atgggcaagc tggcctacga gggcgtgtca tcccgaaaga cctacttctc tgaagaggat
     3661 gtccgtggct gcctggaagc tggcatcaag acagaggaag agtttcaact gcttcagatc
     3721 ttccgcaggg acgccctgag gtttttcctg gccccgtgtg tggaaccagg gcacctgggt
     3781 accttcgtgt tcaccgtgcc cgccatgcag gagtatctgg ctgccctcta catcgtgctt
     3841 ggtttgcgca agacagccct gcagcgggtg ggcaaagaag tggttgaatt tgtgggccgt
     3901 gttggggaag atgtcagcct ggtattgggc attgtggcca agctgttgcc cctgcggatt
     3961 ctgcctctgc tcttcaactt gctcaaggta gttccgcgag tgtttgggcg catggtgagt
     4021 aagagccggg aggcagtggc ccaggccatg gtgctggaga tgttccggga ggaagactac
     4081 tacaatgacg atgttctgga tcagatgggt gccagcatcc tgggtgtgga gggcccccgg
     4141 cgccacccag atgaaccctc tgaggatgaa gtctttgagc tcttccccat gttcatgggc
     4201 ggacttctct ctgcccacaa ccgggcggtg ctggctcagc ttggctgtcc catcaagaac
     4261 ctggatgccc tggagaatgc ccaggccatc aagaagaagc tggggaagct gggtcggcag
     4321 gtgctgcccc cctcggagct tcttgaccat ctcttcttcc actatgagtt ccagaaccag
     4381 cgcttctcag ctgaggtgct gggctcccta cgccagctca atttagcagg ggtgcgcatg
     4441 acacccctca agtgcacagt ggtagcctct gtactgggaa gtggaaggca ccccctggat
     4501 gaggtgaact tggcctcctg ccagctggat cccgctgggc tacacactct catgcctgtc
     4561 ctcctgcgtg cccggaaact ggggttgcaa ctcaacaatc tgggccccga ggcctgcaga
     4621 gacctccgag acctgctctt acacgatcaa tgccagatca ccactcttag gctctccaac
     4681 aacccactga cagcagctgg tgtgggctta ctgatggacg ggctggcagg aaacacttcg
     4741 gtgacacacc tgtctctgct gcacactgac cttggagacg agggactgga actgctggct
     4801 gcccagctgg accgaaacaa acaactgcag gagctgaacg tggcctacaa cggtgctggt
     4861 gacacagtgg ctctggcctt ggctaaggct gctcgggagc acccttccct ggagctgctg
     4921 cacctctact tcaatgagct gagttcagag ggccgccagg tcctgcggga tttggggggc
     4981 tctggtgaag gtggtgcccg ggtcgtagcc tcgctgacag aagggacggc ggtgtctgag
     5041 tactggtcag tgatccttag tgaagtccag cgcaacgtcc acagctggga cccgctccgg
     5101 gtccagaggc atctcaagct gctgctccgt gatctggagg acagccgggg cgccaccctt
     5161 aatccctggc gcaaggctca gcttctgcga gtggagggcg aggtcaagac tcttctggag
     5221 cagctgggag gttctggaca cggatccgac tacaaagacc atgacggtga ttataaagat
     5281 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc
     5341 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     5401 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     5461 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     5521 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     5581 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     5641 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     5701 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     5761 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     5821 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc
     5881 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc
     5941 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc
     6001 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg
     6061 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac
     6121 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag
     6181 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc
     6241 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc
     6301 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc
     6361 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc
     6421 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc
     6481 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag
     6541 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc
     6601 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta
     6661 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     6721 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6781 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6841 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6901 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6961 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     7021 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     7081 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     7141 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7201 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7261 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     7321 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7381 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7441 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7501 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7561 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7621 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7681 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7741 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7801 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7861 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7921 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7981 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8041 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8101 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8161 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8221 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8281 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8341 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8401 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8521 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
     8581 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8641 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8701 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8761 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8821 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8881 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8941 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9001 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9061 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9421 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9721 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9781 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9841 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9901 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9961 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10021 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10081 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10141 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10201 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10261 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10321 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10381 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10441 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10501 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10561 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10621 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10681 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10741 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10801 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10861 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10921 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10981 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    11041 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    11101 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    11161 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
