LOCUS       V021814                11449 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021814
VERSION     V021814
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5491
                     /label="NLRC3(NM_178844)"
                     /note="NLRC3(NM_178844)"
                     /gene="NLRC3"
     CDS             5498..5563
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5608..5819
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5832..6100
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6129..6138
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6159..6821
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6891..6944
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6945..7544
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7545..8132
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8206..8439
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8511..8632
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8651..8786
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8824..8840)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8848..8864
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8872..8902)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8917..8938
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9226..9814)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9985..10845)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10846..10950)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11424..11440
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga ggaagcaaga ggtgcggacg ggcagggagg ccggccaggg
     2341 ccacggtacg ggctccccag ccgagcaggt gaaagccctc atggatctgc tggctgggaa
     2401 gggcagtcaa ggctcccagg ccccgcaggc cctggatagg acaccggatg ccccgctggg
     2461 gccctgcagc aatgactcaa ggatacagag gcaccgcaag gccctgctga gcaaggtggg
     2521 aggtggcccg gagctgggcg gaccctggca caggctggcc tccctcctgc tggtggaggg
     2581 cctgacggac ctgcagctga gggaacacga cttcacacag gtggaggcca cccgcggggg
     2641 cgggcacccc gccaggaccg tcgccctgga ccggctcttc ctgcctctct cccgggtgtc
     2701 tgtcccaccc cgggtctcca tcactatcgg ggtggccggc atgggcaaga ccaccctggt
     2761 gaggcacttc gtccgcctct gggcccatgg gcaggtcggc aaggacttct cgctggtgct
     2821 gcctctgacc ttccgggatc tcaacaccca cgagaagctg tgtgccgacc gactcatctg
     2881 ctcggtcttc ccgcacgtcg gggagcccag cctggcggtg gcagtcccag ccagggccct
     2941 cctgatcctg gacggcttgg atgagtgcag gacgcctctg gacttctcca acaccgtggc
     3001 ctgcacggac ccaaagaagg agatcccggt ggaccacctg atcaccaaca tcatccgtgg
     3061 caacctcttt ccggaagttt ccatctggat cacctcccgt cccagtgcat ctggccagat
     3121 cccagggggc ctggtggacc ggatgacgga gatccggggc tttaacgagg aggagatcaa
     3181 ggtgtgtttg gagcagatgt tccccgagga ccaggccctt ctgggctgga tgctgagcca
     3241 agtgcaggct gacagggccc tgtacctgat gtgcaccgtc ccagccttct gcaggctcac
     3301 ggggatggcg ctaggccacc tgtggcgcag caggacgggg ccccaggatg cagagctgtg
     3361 gcccccgagg accctgtgcg agctctactc atggtacttt aggatggccc tcagcgggga
     3421 ggggcaggag aagggcaagg caagccctcg catcgagcag gtggcccatg gtggccgcaa
     3481 gatggtgggg acattgggcc gtctggcctt ccatgggctg ctcaagaaga aatacgtgtt
     3541 ttacgagcaa gacatgaagg cgtttggtgt agacctcgct ctgctgcagg gcgccccgtg
     3601 cagctgcttc ctgcagagag aggagacgtt ggcatcgtca gtggcctact gcttcaccca
     3661 cctgtccctg caggagtttg tggcagccgc gtattactat ggcgcatcca ggagggccat
     3721 cttcgacctc ttcactgaga gcggcgtatc ctggcccagg ctgggcttcc tcacgcattt
     3781 caggagcgca gcccagcggg ccatgcaggc agaggacggg aggctggacg tgttcctgcg
     3841 cttcctctcc ggcctcttgt ctccgagggt caatgccctc ctggccggct ccctgctggc
     3901 ccaaggcgag caccaggcct accggaccca ggtggctgag ctcctgcagg gctgcctgcg
     3961 ccccgatgcc gcagtctgtg cacgggccat caacgtgttg cactgcctgc atgagctgca
     4021 gcacaccgag ctggcccgca gcgtggagga ggccatggag agcggggccc tggccaggct
     4081 gactggtccc gcgcaccgcg ctgccctggc ctacctcctg caggtgtccg acgcctgtgc
     4141 ccaggaggcc aacctgtccc tgagcctcag ccagggcgtc cttcagagcc tgctgcccca
     4201 gctgctctac tgccggaagc tcaggctgga caccaaccag ttccaggacc ccgtgatgga
     4261 gctgctgggc agcgtgctga gtgggaagga ctgtcgcatt cagaagatca gcttggcgga
     4321 gaaccagatc agtaacaaag gggccaaagc tctggccaga tccctcttgg tcaacagaag
     4381 tctgacctct ctggacctcc gcggtaactc cattggacca caaggggcca aggcgctggc
     4441 agacgctttg aagatcaacc gcaccctgac ctccctgagc ctccagggca acaccgttag
     4501 ggatgatggt gccaggtcca tggctgaggc cttggcctcc aaccggaccc tctccatgct
     4561 gcacctgcag aagaacagca tcgggcccat gggagcccag cggatggcag atgccttgaa
     4621 gcagaacagg agtctgaaag agctcatgtt ctccagtaat agtattggtg atggaggtgc
     4681 caaggccctg gctgaggccc tgaaggtgaa ccagggcctg gagagcctgg acctgcagag
     4741 caattccatc agtgacgcag gagtggcagc actgatgggg gccctctgca ccaaccagac
     4801 cctcctcagc ctcagccttc gagaaaactc catcagtccc gagggagccc aggccatcgc
     4861 tcatgccctc tgcgccaaca gcaccctgaa gaacctggac ctgacagcca acctcctcca
     4921 cgaccagggt gcccgggcca tcgcagtggc agtgagagaa aaccgcaccc tcacctccct
     4981 tcacctgcag tggaacttca tccaggccgg cgctgcccag gccctgggac aagcactaca
     5041 gctcaacagg agcctcacca gcttagattt acaggagaac gccatcgggg atgacggagc
     5101 gtgtgcggtg gcccgtgcac tgaaggtcaa cacagccctc actgctctct atctccaggt
     5161 ggcctcaatt ggtgcttcag gcgcccaggt gctaggggaa gccttggctg tgaacagaac
     5221 cttggagatt ctcgacttaa gaggaaatgc cattggggtg gctggagcca aagccctggc
     5281 aaatgctctg aaggtaaact caagtctccg gagactcaat cttcaagaga attctctggg
     5341 gatggacggg gcgatatgca ttgccacagc actgtctgga aaccacaggc tccagcatat
     5401 caatctccag ggaaaccaca ttggggactc cggggccagg atgatctcag aggccatcaa
     5461 gacaaatgct cccacgtgca ctgttgaaat gggatccgac tacaaagacc atgacggtga
     5521 ttataaagat catgacatcg actacaagga tgacgatgac aagtgagcgg ccgcgaagga
     5581 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     5641 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     5701 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     5761 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     5821 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     5881 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     5941 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     6001 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     6061 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
     6121 cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc
     6181 gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc
     6241 ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc
     6301 cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg
     6361 gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg
     6421 agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc
     6481 gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca
     6541 ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg
     6601 tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg
     6661 tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg
     6721 gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg
     6781 tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt
     6841 ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag
     6901 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc
     6961 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7021 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     7081 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7141 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7201 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7261 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7321 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7381 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7441 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     7501 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt
     7561 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7621 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7681 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7741 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7801 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7861 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7921 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7981 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8041 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga
     8101 cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     8161 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8221 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8461 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8641 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8701 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8761 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     8821 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8881 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8941 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9661 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10921 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10981 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    11041 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    11101 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11161 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11221 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11281 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11341 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11401 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
