LOCUS       V034105                10354 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034105
VERSION     V034105
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5208
                     /label="NFKB1(NM_003998)"
                     /note="NFKB1(NM_003998)"
                     /gene="NFKB1"
     CDS             5215..5280
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5324..5535
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5548..5816
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5843..6442
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6449..7037
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7111..7344
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7416..7537
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7556..7691
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7729..7745)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7753..7769
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7777..7807)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7822..7843
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8131..8719)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8890..9750)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9751..9855)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10329..10345
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcagaa gatgatccat atttgggaag gcctgaacaa
     2341 atgtttcatt tggatccttc tttgactcat acaatattta atccagaagt atttcaacca
     2401 cagatggcac tgccaacagc agatggccca taccttcaaa tattagagca acctaaacag
     2461 agaggatttc gtttccgtta tgtatgtgaa ggcccatccc atggtggact acctggtgcc
     2521 tctagtgaaa agaacaagaa gtcttaccct caggtcaaaa tctgcaacta tgtgggacca
     2581 gcaaaggtta ttgttcagtt ggtcacaaat ggaaaaaata tccacctgca tgcccacagc
     2641 ctggtgggaa aacactgtga ggatgggatc tgcactgtaa ctgctggacc caaggacatg
     2701 gtggtcggct tcgcaaacct gggtatactt catgtgacaa agaaaaaagt atttgaaaca
     2761 ctggaagcac gaatgacaga ggcgtgtata aggggctata atcctggact cttggtgcac
     2821 cctgaccttg cctatttgca agcagaaggt ggaggggacc ggcagctggg agatcgggaa
     2881 aaagagctaa tccgccaagc agctctgcag cagaccaagg agatggacct cagcgtggtg
     2941 cggctcatgt ttacagcttt tcttccggat agcactggca gcttcacaag gcgcctggaa
     3001 cccgtggtat cagacgccat ctatgacagt aaagccccca atgcatccaa cttgaaaatt
     3061 gtaagaatgg acaggacagc tggatgtgtg actggagggg aggaaattta tcttctttgt
     3121 gacaaagttc agaaagatga catccagatt cgattttatg aagaggaaga aaatggtgga
     3181 gtctgggaag gatttggaga tttttccccc acagatgttc atagacaatt tgccattgtc
     3241 ttcaaaactc caaagtataa agatattaat attacaaaac cagcctctgt gtttgtccag
     3301 cttcggagga aatctgactt ggaaactagt gaaccaaaac ctttcctcta ctatcctgaa
     3361 atcaaagata aagaagaagt gcagaggaaa cgtcagaagc tcatgcccaa tttttcggat
     3421 agtttcggcg gtggtagtgg tgctggagct ggaggcggag gcatgtttgg tagtggcggt
     3481 ggaggagggg gcactggaag tacaggtcca gggtatagct tcccacacta tggatttcct
     3541 acttatggtg ggattacttt ccatcctgga actactaaat ctaatgctgg gatgaagcat
     3601 ggaaccatgg acactgaatc taaaaaggac cctgaaggtt gtgacaaaag tgatgacaaa
     3661 aacactgtaa acctctttgg gaaagttatt gaaaccacag agcaagatca ggagcccagc
     3721 gaggccaccg ttgggaatgg tgaggtcact ctaacgtatg caacaggaac aaaagaagag
     3781 agtgctggag ttcaggataa cctctttcta gagaaggcta tgcagcttgc aaagaggcat
     3841 gccaatgccc ttttcgacta cgcggtgaca ggagacgtga agatgctgct ggccgtccag
     3901 cgccatctca ctgctgtgca ggatgagaat ggggacagtg tcttacactt agcaatcatc
     3961 caccttcatt ctcaacttgt gagggatcta ctagaagtca catctggttt gatttctgat
     4021 gacattatca acatgagaaa tgatctgtac cagacgccct tgcacttggc agtgatcact
     4081 aagcaggaag atgtggtgga ggatttgctg agggctgggg ccgacctgag ccttctggac
     4141 cgcttgggta actctgtttt gcacctagct gccaaagaag gacatgataa agttctcagt
     4201 atcttactca agcacaaaaa ggcagcacta cttcttgacc accccaacgg ggacggtctg
     4261 aatgccattc atctagccat gatgagcaat agcctgccat gtttgctgct gctggtggcc
     4321 gctggggctg acgtcaatgc tcaggagcag aagtccgggc gcacagcact gcacctggct
     4381 gtggagcacg acaacatctc attggcaggc tgcctgctcc tggagggtga tgcccatgtg
     4441 gacagtacta cctacgatgg aaccacaccc ctgcatatag cagctgggag agggtccacc
     4501 aggctggcag ctcttctcaa agcagcagga gcagatcccc tggtggagaa ctttgagcct
     4561 ctctatgacc tggatgactc ttgggaaaat gcaggagagg atgaaggagt tgtgcctgga
     4621 accacgcctc tagatatggc caccagctgg caggtatttg acatattaaa tgggaaacca
     4681 tatgagccag agtttacatc tgatgattta ctagcacaag gagacatgaa acagctggct
     4741 gaagatgtga agctgcagct gtataagtta ctagaaattc ctgatccaga caaaaactgg
     4801 gctactctgg cgcagaaatt aggtctgggg atacttaata atgccttccg gctgagtcct
     4861 gctccttcca aaacacttat ggacaactat gaggtctctg ggggtacagt cagagagctg
     4921 gtggaggccc tgagacaaat gggctacacc gaagcaattg aagtgatcca ggcagcctcc
     4981 agcccagtga agaccacctc tcaggcccac tcgctgcctc tctcgcctgc ctccacaagg
     5041 cagcaaatag acgagctccg agacagtgac agtgtctgcg acagcggcgt ggagacatcc
     5101 ttccgcaaac tcagctttac cgagtctctg accagtggtg cctcactgct aactctcaac
     5161 aaaatgcccc atgattatgg gcaggaagga cctctagaag gcaaaattgg atccgactac
     5221 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     5281 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5341 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5401 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5461 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5521 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5581 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5641 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5701 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5761 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5821 ctgtgaccgg cgcctacgct agatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5881 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5941 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     6001 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     6061 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     6121 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     6181 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     6241 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6301 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6361 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6421 gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6481 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6541 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6601 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6661 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6721 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6781 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6841 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6901 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6961 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7021 ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7081 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat
     7141 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7321 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7381 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7561 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7621 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7681 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt
     7741 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7801 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7861 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8821 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8881 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9001 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9721 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9781 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9841 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9901 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     9961 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10021 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10081 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10201 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10261 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10321 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
