LOCUS       V038162                11317 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038162
VERSION     V038162
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5377
                     /label="NAT10(NM_024662)"
                     /note="NAT10(NM_024662)"
                     /gene="NAT10"
     CDS             5384..5410
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5414..5440
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5444..5470
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5515..5726
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5739..6007
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6042..6749
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6759..6812
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6813..7412
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7413..8000
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8074..8307
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8379..8500
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8519..8654
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8692..8708)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8716..8732
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8740..8770)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8785..8806
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9094..9682)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9853..10713)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10714..10818)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11292..11308
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcatcg gaaaaaggtg gataaccgaa tccggattct
     2341 cattgagaat ggagtagctg agcggcaaag atctctcttt gttgtagttg gggatcgagg
     2401 aaaagatcag gtggtaatac ttcatcacat gttatccaaa gcaactgtga aggctcggcc
     2461 ttcagtgctg tggtgttata agaaagagct ggggtttagc agtcaccgga agaaaagaat
     2521 gcgacagctg cagaagaaaa taaagaatgg aacactgaac ataaagcagg acgacccctt
     2581 tgaactcttc atagcagcca caaacattcg ctactgctac tacaacgaga cccacaagat
     2641 cctgggcaat accttcggca tgtgtgtgct gcaggatttt gaagccttaa ctccaaactt
     2701 gctggccagg actgtagaaa cagtggaagg tggtgggcta gtggtcatcc tcctacggac
     2761 catgaactca ctcaagcaat tgtacacagt gactatggat gtgcattcca ggtacagaac
     2821 tgaggcccat caggatgtgg tgggaagatt taatgaaagg tttattctgt ctctggcctc
     2881 ttgtaagaag tgtctcgtca ttgatgacca gctcaacatc ctgcccatct cctcccacgt
     2941 tgccaccatg gaggccctgc ctccccagac tccggatgag agtcttggtc cttctgatct
     3001 ggagctgagg gagttgaagg agagcttgca ggacacccag cctgtgggtg tgttggtgga
     3061 ctgctgtaag actctagacc aggccaaagc tgtcttgaaa tttatcgagg gcatctctga
     3121 aaagaccctg aggagtactg ttgcactcac agctgctcga ggacggggaa aatctgcagc
     3181 cctgggattg gcgattgctg gggcggtggc atttgggtac tccaatatct ttgttacctc
     3241 cccaagccct gataacctcc atactctgtt tgaatttgta tttaaaggat ttgatgctct
     3301 gcaatatcag gaacatctgg attatgagat tatccagtct ctaaatcctg aatttaacaa
     3361 agcagtgatc agagtgaatg tatttcgaga acacaggcag actattcagt atatacatcc
     3421 tgcagatgct gtgaagctgg gccaggctga actagttgtg attgatgaag ctgccgccat
     3481 ccccctcccc ttggtgaaga gcctacttgg cccctacctt gttttcatgg catccaccat
     3541 caatggctat gagggcactg gccggtcact gtccctcaag ctaattcagc agctccgtca
     3601 acagagcgcc cagagccagg tcagcaccac tgctgagaat aagaccacga cgacagccag
     3661 attggcatca gcgcggacac tgtatgaggt ttccctccag gagtcaatcc gatacgcccc
     3721 tggggatgca gtggagaagt ggctgaatga cttgctgtgc ctggattgcc tcaacatcac
     3781 tcggatagtc tcaggctgcc ccttgcctga agcttgtgaa ctgtactatg ttaatagaga
     3841 taccctcttt tgctaccaca aggcctctga agttttcctc caacggctta tggccctcta
     3901 cgtggcttct cactacaaga actctcccaa tgatctccag atgctctccg atgcacctgc
     3961 tcaccatctc ttctgccttc tgcctcccgt gccccccacc cagaatgccc ttccagaagt
     4021 gcttgctgtt atccaggtgt gccttgaagg ggagatttct cgccagtcca tcttgaacag
     4081 tctgtctcga ggcaagaagg cttcagggga cctgattcca tggacagtgt cagaacagtt
     4141 ccaagatcca gactttggtg gtctgtctgg tggaagggtc gttcgcattg ctgttcaccc
     4201 agattatcaa gggatgggct atggcagccg tgctctgcag ctgctgcaga tgtactatga
     4261 aggcaggttt ccttgtctgg aggaaaaggt ccttgagaca ccacaggaaa ttcacaccgt
     4321 aagcagcgag gctgtcagct tgttggaaga ggtcatcact ccccggaagg acctgcctcc
     4381 tttactcctc aaattgaatg agaggcctgc cgaacgcctg gattacctgg gtgtttccta
     4441 tggcttgacc cccaggctcc tcaagttctg gaaacgagct ggatttgttc ctgtttatct
     4501 gagacagacc ccgaatgacc tgaccggaga gcactcgtgc atcatgctga agacgctcac
     4561 tgatgaggat gaggctgacc agggaggctg gcttgcagcc ttctggaaag atttccgacg
     4621 gcggttccta gccttgctct cctaccagtt cagtaccttc tctccttccc tggctctgaa
     4681 catcattcag aacaggaaca tggggaagcc agcccagcct gccctgagcc gggaggagct
     4741 ggaagcactc ttcctcccct atgacctgaa gcggctggag atgtattcac ggaatatggt
     4801 ggactatcac ctcatcatgg acatgatccc ggccatctct cgcatctatt tcctgaacca
     4861 gctgggggac ctggccctgt ctgcggctca gtcggctctt ctcttgggga ttggcctgca
     4921 gcataagtct gtggaccagc tggaaaagga gattgagctg ccctcgggcc agttgatggg
     4981 acttttcaac cggatcatcc gcaaagttgt gaagctattt aatgaagttc aggaaaaggc
     5041 cattgaggag cagatggtgg cagcgaagga tgtggtcatg gagcccacga tgaagaccct
     5101 cagtgacgac ctagatgaag cagcaaagga atttcaggag aaacacaaga aggaagtagg
     5161 gaagctgaag agcatggacc tctctgaata cataatccgt ggggacgatg aagagtggaa
     5221 tgaagttttg aacaaagctg ggccgaacgc ctcgatcatc agcctgaaaa gtgacaagaa
     5281 aaggaagtta gaggccaaac aagaacccaa acagagcaag aagttgaaga acagagagac
     5341 aaagaacaaa aaagatatga aactgaagcg gaagaaagga tcctacccat acgacgtacc
     5401 tgattatgca acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc
     5461 agactacgca taggcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag
     5521 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
     5581 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     5641 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     5701 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
     5761 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     5821 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     5881 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     5941 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     6001 acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggtgagc aagggcgagg
     6061 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     6121 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6181 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     6241 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     6301 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6361 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     6421 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     6481 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     6541 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     6601 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     6661 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     6721 ccaccggcgg catggacgag ctgtacaagg gatctcgcga gggcagagga agtcttctaa
     6781 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc
     6841 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6901 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6961 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     7021 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     7081 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     7141 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     7201 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     7261 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7321 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7381 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg
     7441 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7501 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7561 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7621 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7681 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7741 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7801 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7861 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7921 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct
     7981 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     8041 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8101 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8161 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8221 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8281 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8341 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8461 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     8521 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8581 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8641 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
     8701 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8761 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8821 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8881 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10861 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10921 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10981 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    11041 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    11101 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    11161 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11221 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    11281 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
