LOCUS       V024194                12701 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024194
VERSION     V024194
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6744
                     /label="Mug1(NM_008645)"
                     /note="Mug1(NM_008645)"
                     /gene="Mug1"
     CDS             6751..6816
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6860..7071
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7084..7352
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7381..7390
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7411..8073
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8143..8196
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8197..8796
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8797..9384
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9458..9691
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9763..9884
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9903..10038
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10076..10092)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10100..10116
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10124..10154)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10169..10190
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10478..11066)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11237..12097)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12098..12202)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12676..12692
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ggaagagcag acgggctcag
     2341 ttgtgccttt tttcggttct ccttgccttc ctgcattctg cttctttact caacggagac
     2401 tcgaagtata tggtgctggt cccttctcag ctctacactg agacccctga gaaaatctgc
     2461 ctccatctat accagctgaa tgaaacagtg accgtcacag cttccttggt atctcaatca
     2521 ggaaggaaaa acctgttcga tgagctggtg cttgacaagg acttgttcca atgtgtttcc
     2581 ttcattatcc ctagactctc ctcttctgat gaggaggatt ttctctacgt ggacatcaaa
     2641 gggccaaccc acgaattcag caaaaggaaa gcagtgcttg tgaagaacaa agaaagtgtt
     2701 gtctttgtgc agacagataa gcccgtgtac aagccaggac aatcagttaa atttcgggtt
     2761 gtctctatgg ataaaatgct acgtcccctg aatgagttgc ttcctctggc ttacattgag
     2821 gatccgaaaa agaaccgaat tatgcagtgg agggatatta agactgagaa tgggcttaag
     2881 caaatgtcct tcagcctggc agcagagccc attcagggcc cctacaagat agtggtgcac
     2941 aaagagtcag gggagaagga agaacactcc tttactgtga tggaatttgt gcttcccaga
     3001 tttaatgtcg acctgaaggt cccaaatgcc atgtctgtga atgatgaagt gctcagtgtg
     3061 actgcatgtg ggaaatatac ctatgggaag cctgttccag gacatgtgaa gataaacgtc
     3121 tgccgtgaga ctgagactgg atgcagagaa gtcaattctc agctagacaa caatggctgc
     3181 agtacacaag aagtgaacat cactgagctc caatcaaaga aaaggaatta tgaggttcag
     3241 cttttccatg tgaatgcaac tgttacagaa gaagggacag gattggagtt cagtcgatct
     3301 ggaacaacta aaattgaaag aatcacaaac aagctcatat ttctgaaggc agattcacac
     3361 ttcagacatg gaattccatt ctttgtgaaa gtccgcctag tggatatcaa gggagaccct
     3421 atcccaaatg agaaagtctt catcaaagca caagaactta gctataccag tgctactacc
     3481 actgatcagc atggcctggc agagttctcc atagatacca catgcatctc gggctcttcc
     3541 ctccatatca aagtcaacca caaagaggaa gattcatgtt cctatttcta ttgcatggag
     3601 gaaagacatg caagtgcaaa gcatgtggcc tatgctgttt actccctcag caagagctac
     3661 atctaccttg acacagagac cagcagcatc ttgccctgca accagattca cacagttcag
     3721 gcacatttta ttctgaaggg ggacttggga gtgctgaaag agctcatttt ctactacctg
     3781 gtcatggcgc agggaagcat catccagact ggaaaccata ctcaccaggt ggagccagga
     3841 gaagctccag taaaaggaaa atttgccttg gagatccctg tggagtttag catggtcccc
     3901 atggctaaaa tgctcatcta cacgatcttg cctgatggag aagtgattgc agattctgta
     3961 aactttgaaa ttgaaaagtg tcttcgcaac aaagtggacc tgcgcttcag cacttctcaa
     4021 agtcttcccg cctcacaaac ccgtctgcag gtcacagctt ctcctcagtc cctctgtggc
     4081 ctgagagctg tggaccagag tgtgctactc ctgaaacccg agtctgagct ctccccttcc
     4141 tggatataca atctgccagg tatgcagcaa aacaaattcg ttccaagttc ccgtctgtct
     4201 gaagaccaag aagactgtat actgtacagt tcatggttgg ctgagaagca cacaaactta
     4261 gtaccacatg gaactgagaa ggatgtctat agatatgtgg aggacatggg tttaacagca
     4321 ttcaccaact tgatgatcaa acttcctata atttgttttg actatggcat ggttccaata
     4381 tcagcacctc gtgtggaatt tgatcttgca tttacccctg aaatttcttg gagtttacgc
     4441 acaacattga gtaaaagacc tgaagagcca ccacgtaaag atccatcatc gaatgatcca
     4501 cttacagaga ccattcgaaa atattttcct gaaacctggg tctgggatat agtcacagta
     4561 aactccacag gactggctga agtggaaatg actgtccctg acaccatcac tgaatggaag
     4621 gcaggagctc tctgcttgtc caatgacact ggccttggcc tctcttctgt ggtacctctc
     4681 caagccttca agcccttctt tgtggaggtc tcattgccct attctgtggt ccgtggagaa
     4741 gccttcatgc tcaaggccac tgtgatgaac tatctcccca caagcatgca gatgagtgtg
     4801 cagctggaag cctctcctga tttcacagct gtcccagtgg gagacgacca agattcttac
     4861 tgcctcagtg ccaatgggag gcacacctca tcctggctgg taactcccaa gtctttaggg
     4921 aatgtgaatt tctctgtgtc tgcggaagca caacagtcct cagagccctg tggttctgag
     4981 gtggccacag ttcctgaaac tgggagaaag gacacagtag tcaaagtcct gatagttgag
     5041 cctgaaggaa tcaagcaaga gcataccttc agctcactgt tctgtgcatc agatgctgag
     5101 atatctgaaa aaatgtccct ggtcctccca ccaacagtag tgaaagattc agcaagagcc
     5161 catttctctg tgatgggtga tatcttgagt tcagccataa gaaacacaca aaaccttctc
     5221 catatgccct atggctgtgg ggagcagaac atggtccttt ttgctcccaa catctacgta
     5281 ctgaaatatc tgaatgaaac ccaacagctg actcagaaga tcaagactaa agcccttggg
     5341 ttcctcagag ctggttatca gagagagctg aactacaaac acaaggatgg ctcctacagt
     5401 gccttcgggg atcaaaatgg cgaaagagaa ggaaacactt ggctcacagc ctttgtgctc
     5461 aagtcttttg cccaagctcg agccttcatc ttcatcgatg aatcacacat cacccatgcc
     5521 ttcacctggc tctcccaaaa gcagaaggac aatggctgct tccggagctc cggatcattg
     5581 ttcaacaatg ccatgaaggg gggagtagat gatgaaatga ccctctctgc ctacataacc
     5641 atggcccttc tggaaagttc actcccagcc acgcatcctg ttgtctccaa agccctgagc
     5701 tgcctggagt catcctggaa gaccatagaa caagaacgaa atgccagctt tgtgtacacc
     5761 aaggctttga tggcctatgc ttttgctctg gcggggaacc agaataagag agatgaaatc
     5821 ctgaagtcac ttgatgagga agctataaaa gaaaacaact ctatccactg gaaaagacct
     5881 cagaaatcca ggaaatctga acaccatttg tacaaacccc aggcttcctc tgctgaagta
     5941 gagatgaatg catatgtggt cctggctcgc ctcactgccc agccagcccc atcccctgag
     6001 gatctgactt tgtcaatgag caccatcatg tggctcacaa agcaacagaa ttccaatggt
     6061 ggcttctcct ccacacagga cactgtggtg gcccttgatg ctctgtccaa atatggagca
     6121 gtgacttttt cgagaagtca gaaaacgact ttggtgacta tccaatctac agggtcattt
     6181 tcccaaaagt tccaagtgga gaacagtaat cgcctgttac tacagcaggt cgcattacca
     6241 gacattcctg gggactatac catcagtgtg tcaggggaag gatgtgtgta tgctcagact
     6301 atgctgagat acaacatgca cttggagaag cagctgtctg catttgctat atgggtacag
     6361 acagtacctc taacttgtaa taaccccaaa ggccacaaca gcttccagat ctcactagaa
     6421 atcagttaca caggcagccg gccagcctcc aacatggtga ttgctgatgt gaagatgcta
     6481 tctggtttca tcccattgaa accaacagtg aaaaagcttg aaagattaga gcacgtgagc
     6541 agaacagaag tgagcaacaa caatgtctta atatatttgg atcaggtgac caatcagaca
     6601 ctggccttct ccttcatcat tcaacaagat atcccagtaa ggaacctgca gcctgccatt
     6661 gtgaaagtct atgactacta tgagacagat gaaatggctt ttgctgaata cagcagcccc
     6721 tgcagcacag acaaacaaaa tgttggatcc gactacaaag accatgacgg tgattataaa
     6781 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     6841 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     6901 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     6961 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     7021 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     7081 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     7141 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     7201 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     7261 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     7321 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     7381 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     7441 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     7501 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     7561 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     7621 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     7681 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     7741 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     7801 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     7861 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     7921 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     7981 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     8041 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     8101 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     8161 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     8221 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     8281 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     8341 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     8401 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     8461 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     8521 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     8581 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     8641 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     8701 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     8761 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     8821 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8881 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8941 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     9001 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     9061 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     9121 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     9181 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     9241 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     9301 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     9361 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     9421 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9481 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     9541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9661 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     9721 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     9781 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9841 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     9901 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9961 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10021 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
    10081 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10141 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10201 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10261 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10321 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10381 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    12241 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    12301 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    12361 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    12421 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    12481 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    12541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    12601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    12661 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
