LOCUS V036384 15962 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036384 VERSION V036384 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..9963 /label="Mtor(NM_020009)" /note="Mtor(NM_020009)" /gene="Mtor" CDS 9970..9996 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 10000..10026 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 10030..10056 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 10121..10332 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 10345..10613 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 10642..10651 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 10672..11334 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 11404..11457 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 11458..12057 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 12058..12645 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 12719..12952 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 13024..13145 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 13164..13299 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(13337..13353) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 13361..13377 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(13385..13415) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 13430..13451 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(13739..14327) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(14498..15358) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(15359..15463) /gene="bla" /label="AmpR promoter" primer_bind 15937..15953 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ttgggacggg tcctgccgtg 2341 gccaccgcca gtgccgccac atctagcaac gtgagcgtcc tgcagcagtt cgccagtgga 2401 ctgaagagcc ggaatgagga gaccagggcc aaagcagcca aggagctcca gcactacgtc 2461 accatggagc ttcgagagat gagtcaggag gagtctactc gcttctatga ccagctgaac 2521 catcacattt ttgaactggt ttccagctca gatgccaatg agaggaaggg tggcatcttg 2581 gccatcgcca gcctcatagg agtggaaggt gggaattcca ccagaattgg cagatttgcc 2641 aactaccttc gaaacctcct cccctcaagc gatccagttg tcatggaaat ggcgtccaag 2701 gccattggcc gcctggcgat ggcaggggac actttcactg ctgaatatgt ggagtttgaa 2761 gtgaagcgag ccttggagtg gctgggtgct gaccgaaatg agggccggag acatgccgct 2821 gtcctcgttc tccgtgagct ggccatcagt gtccccacct tcttcttcca gcaagttcag 2881 cccttctttg acaacatttt tgtggctgtg tgggacccca agcaggccat ccgggaaggc 2941 gctgtagcgg cccttcgtgc ctgtctgatt ctcaccacgc agcgggaacc aaaggaaatg 3001 cagaagcctc agtggtaccg gcacacattt gaagaagcag agaaaggttt tgatgagacc 3061 ctggccaaag agaagggtat gaatcgagat gatcgaatcc acggagcctt gctgatcctc 3121 aacgagctag ttcgtatcag cagcatggag ggagagcgtc tgagagaaga gatggaggag 3181 atcacccagc agcagctggt gcatgacaag tactgcaaag acctcatggg cttcgggacc 3241 aagcctcggc acatcacgcc cttcaccagt ttccaggctg tgcagcccca gcagccgaac 3301 gccttggtgg gactgctggg gtacagctcc cctcaaggcc tgatgggatt tgggacgtcc 3361 cccagccctg ccaagtccac tctggtggaa agccgctgtt gcagagactt gatggaagag 3421 aaatttgatc aggtgtgcca gtgggtgctg aagtgcagga gcagcaagaa ctcgctgatc 3481 cagatgacaa tccttaacct gctgccccgc ctggctgcat tccgaccgtc cgccttcaca 3541 gatacccagt acctccagga caccatgaac catgtcctga gctgtgtcaa gaaggagaag 3601 gaacggactg cggcgttcca ggccctgggg ctgctttctg tggccgtgag gtcggagttt 3661 aaggtctact tgccccgtgt acttgacatc atccgagcag cgcttcctcc aaaggacttt 3721 gcccacaaga ggcagaaaac cgtgcaggtg gatgccaccg tattcacgtg catcagcatg 3781 ttggcacgag caatggggcc gggcatccag caggacatca aggagctgct ggagcccatg 3841 ttggcagtgg gcctgagccc cgcgctcact gctgtgctct atgacctgag ccggcagatt 3901 ccacagctga agaaagatat tcaggacggc cttctgaaga tgctgtccct ggtccttatg 3961 cacaaacccc tccggcaccc aggcatgccc aaaggcctgg ctcaccagct ggcttcccct 4021 ggtctcacca ccctccctga ggccagcgac gtggccagca tcactcttgc ccttcgaacc 4081 cttggcagct ttgaatttga aggccactct ctgacccagt tcgtccgaca ctgcgcagat 4141 cacttcctga acagcgagca caaggagatc cgcatggaag ctgctcgcac ctgctcccgc 4201 ctgctcacac cctccatcca cctcatcagc ggccatgccc acgtggttag ccagactgca 4261 gtgcaggtgg tggcagatgt gctcagcaag ctgcttgtgg ttggcataac agatcctgac 4321 cctgatatcc gctactgtgt cttggcatcc ctggacgagc gctttgatgc ccacctggcc 4381 caggcagaaa acttacaagc tctgtttgtg gctctgaatg accaggtctt tgagatccgc 4441 gagctggcca tctgcactgt gggccgacta agcagcatga acccagcctt cgtcatgcct 4501 ttcctgcgca agatgctcat ccagatcctg acagagctgg agcacagcgg cattgggaga 4561 atcaaggagc agagcgcccg catgctgggg cacctggtgt ccaacgcccc ccggctcatc 4621 cgcccctaca tggagcctat cctgaaggct ttaattttga aactgaaaga tccagaccct 4681 gacccaaacc cgggcgtgat caataacgtg ttggccacta taggagaact ggctcaggtg 4741 agcggcctgg aaatgcggaa gtgggtggac gagctcttta tcatcatcat ggacatgctg 4801 caggactcct ccctgctggc caaaaggcag gtggctttgt ggaccctggg acagttggtg 4861 gccagcactg gctatgtggt ggagccctac aggaagtacc ccactttgct tgaagtgctg 4921 ctgaatttcc tgaagacgga gcagaaccag ggcactcgga gagaggctat ccgagtgttg 4981 gggctccttg gggctttgga tccctacaag cacaaagtga acatcggcat gatcgaccag 5041 tcccgggacg cttccgctgt cagcctgtca gagtccaagt caagtcagga ttcctctgac 5101 tacagcacca gtgaaatgct ggtcaacatg ggaaacctgc ccctggacga gttctacccc 5161 gctgtgtcca tggtggcctt gatgcggatc ttccgagatc aatccctctc tcaccaccac 5221 accatggtgg tgcaggccat caccttcatc ttcaagtccc tggggctcaa gtgtgtgcag 5281 ttcctgcccc aggtcatgcc cacattcctt aatgtcatcc gagtctgtga tggggccatc 5341 cgggaatttc tgttccagca gctggggatg ctggtgtcct ttgtgaagag ccacatccgt 5401 ccctacatgg atgaaatagt cactctcatg agagagtttt gggtcatgaa cacgtcaatc 5461 cagagcacaa tcattcttct cattgagcag attgtggtgg ctctcggagg ggaatttaag 5521 ctttatctgc cccagttgat cccacacatg ctgcgggtct tcatgcatga caacagccaa 5581 ggccgaatcg tctccatcaa gctgttagcc gcgatccagc tgtttggcgc caacctggat 5641 gactatctgc acttgttgtt gcctccgatt gtgaaattgt ttgatgcccc tgaagtcccg 5701 ctgccatcaa gaaaggcagc gctggagacg gtggaccgcc tgacagagtc cctagacttc 5761 actgactacg cctcccgcat cattcaccca atagttcgta cgctagacca gagcccagag 5821 ctgcgctcca cagccatgga cactctgtct tcgcttgtct ttcaactggg gaagaagtac 5881 cagatcttca ttccaatggt gaataaagtc ctcgtgcgac accggatcaa ccaccagcgc 5941 tatgatgtgc ttatctgcag aatcgtcaag gggtacacac ttgctgatga ggaagaagac 6001 cctttgattt accagcatcg aatgctaagg agcagccagg gagatgccct ggccagtgga 6061 ccagttgaga caggacccat gaagaaactg catgtcagca ccatcaacct ccaaaaggcc 6121 tggggagctg ccagaagggt ctccaaggac gactggctgg agtggctgag gcgcttgagt 6181 ctggagcttc tgaaggactc ctcatcgccc tccctgcgct catgctgggc cctggcgcag 6241 gcctacaacc ccatggccag ggatctcttc aatgctgcct ttgtgtcctg ctggtctgag 6301 ctgaatgaag accagcaaga tgagctcatc aggagtattg agttggctct cacttctcaa 6361 gacattgctg aagtcacaca aaccctcctg aacttggctg agttcatgga acacagtgac 6421 aagggccccc tgccgctgag agatgacaat ggcatcgtgc tcctgggtga gagagctgcc 6481 aagtgccggg catatgccaa agcactgcac tacaaagaac tggagttcca gaaagggccc 6541 acgcctgcca tacttgagtc cctcatcagc attaacaaca agctccagca gcctgaggca 6601 gcttctgggg tgttggaata cgccatgaaa cacttcggag agctggagat ccaggccacc 6661 tggtatgaga agctgcatga gtgggaggat gctctcgtgg cctacgacaa gaagatggac 6721 acaaacaagg aagacccgga gctgatgctg ggccgaatgc gctgcctcga ggccttgggg 6781 gaatggggcc agcttcatca gcagtgctgt gaaaagtgga ctctggttaa tgatgagacc 6841 caggctaaga tggcccggat ggctgctgct gcagcgtggg gtttaggtca gtgggacagc 6901 atggaggagt acacctgcat gatcccacgg gacacccacg atggagcctt ttacagggca 6961 gtgttggctc tacatcagga tctcttctcc ttggcccagc agtgcattga caaggccagg 7021 gacctgctgg atgcagagct gactgccatg gcaggagaga gctacagccg agcctatggg 7081 gccatggttt cttgccacat gctgtccgag ctggaagagg ttatccagta caaacttgtc 7141 cctgagcgtc gggagatcat ccggcagatc tggtgggaga gactgcaggg ctgccagcgt 7201 attgttgagg actggcagaa aatcctcatg gtccggtccc ttgtggtcag ccctcatgag 7261 gacatgagaa cctggctcaa gtacgcaagc ctgtgtggca agagtggcag actggctctt 7321 gctcataaaa ccttagtgtt gctcttggga gttgatccat ctcggcaact tgaccatcct 7381 ctgccaaccg ctcaccctca agtgacctat gcctacatga agaacatgtg gaaaagtgct 7441 cggaagattg acgccttcca gcacatgcaa cactttgtgc agaccatgca gcagcaggcc 7501 cagcatgcca tcgccacaga ggaccagcag cacaagcagg agctgcataa gctcatggcc 7561 aggtgttttc tgaaacttgg ggagtggcag ctgaacctcc agggcatcaa cgagagcacc 7621 atccccaagg tgctacagta ctacagtgcc gccacagagc atgaccgcag ctggtacaag 7681 gcttggcatg catgggcagt gatgaacttc gaagcagtgc tacactacaa acatcagaac 7741 caagcccgtg atgagaagaa gaagctgcgt catgccagcg gggccaacat caccaatgcc 7801 accactgcag ccaccactgc agcctctgct gctgctgcca ccagcacaga gggcagcaac 7861 agtgagagtg aagctgagag caatgagaac agccccaccc cgtcccctct gcagaagaag 7921 gtcactgagg atttatccaa aactctcttg ttgtacactg tccctgctgt tcaaggcttc 7981 ttccgttcta tctccttgtc aagaggcaac aacctccagg atacactaag agtcctcacc 8041 ctgtggtttg attatggtca ctggccagat gtcaatgaag ccttggtgga aggggtgaag 8101 gccatacaga ttgacacttg gttacaggtt atacctcagc tcattgcaag aattgacaca 8161 cccagaccct tggtgggccg gctcattcac cagcttctca cagatattgg tcggtaccac 8221 ccacaggccc tcatctaccc cctgacggtg gcttctaagt ctaccaccac agcccgtcac 8281 aatgcagcca acaagatctt gaagaacatg tgtgaacaca gcaacacgct ggtccagcag 8341 gccatgatgg tgagtgaaga gctgattcgg gtagccatcc tctggcatga gatgtggcat 8401 gaaggcctgg aagaggcatc tcgcttgtac tttggggaga ggaacgtgaa aggcatgttt 8461 gaggtgctgg agcccctgca tgctatgatg gaacggggtc cccagactct gaaggaaaca 8521 tcctttaatc aggcatatgg ccgagattta atggaggcac aagaatggtg tcgaaagtac 8581 atgaagtcgg ggaacgtcaa ggacctcacg caagcctggg acctctacta tcacgtgttc 8641 agacggatct caaagcagct accccagctc acatccctgg agctgcagta tgtgtccccc 8701 aaacttctga tgtgccgaga ccttgagttg gctgtgccag gaacatacga ccccaaccag 8761 ccaatcattc gcattcaatc catagccccg tctttgcaag tcatcacatc caagcagagg 8821 cctcggaagc tgactctgat gggcagcaat gggcatgagt ttgttttcct cctgaaaggc 8881 catgaagatc tgcggcagga tgaacgagtg atgcagctct ttggcctggt gaacacactc 8941 ctagccaatg accccacttc tcttcgcaag aacctcagca tccagagata cgctgtcatc 9001 cctctgtcca ccaactcggg cctcattggc tgggtgcccc actgtgacac actgcatgcc 9061 ctcatccggg actacagaga gaagaagaag atcctgttga acatcgagca tcgcatcatg 9121 ctgcggatgg ctcctgacta tgaccacctg acgttgatgc agaaggtaga ggtgtttgag 9181 catgctgtca acaacacagc tggggacgac ctggccaagc tactgtggct aaaaagcccc 9241 agctcggagg tgtggtttga ccgaagaacc aactataccc gctccctggc cgttatgtcg 9301 atggtcggat acattttagg ccttggagac aggcacccat ccaatctgat gctggaccgg 9361 ctgagtggga agatcctgca cattgacttt ggggactgct ttgaggtcgc tatgaccaga 9421 gagaaatttc cagaaaagat tccatttaga ctaacaagaa tgttgaccaa tgctatggag 9481 gttacgggtc tggatggcaa ctacagaacc acatgccaca ccgtgatgga agtgctccgg 9541 gaacacaagg acagtgtcat ggctgtgctg gaagcctttg tctatgaccc actgctcaac 9601 tggaggctga tggacacaaa taccaaaggc aataagcggt cccggacaag gacagactcc 9661 tactctgccg gccagtcagt agaaattttg gacggtgtag aacttggaga accagcccat 9721 aagaaagcag ggaccactgt gccagaatcc atccattcat tcattggaga cggtttggtg 9781 aaaccagaag ccttaaacaa gaaagctatt cagattatta acagggttcg agataagctc 9841 actggtcggg atttctctca tgatgacact ttggatgttc caacccaagt ggagctgctt 9901 atcaagcagg caacatctca cgagaacctc tgccagtgct acattggctg gtgtcccttc 9961 tggctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 10021 tatgctagct atccttatga tgtcccagac tacgcatagg gatccctgcg atatccagcg 10081 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 10141 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 10201 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 10261 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 10321 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 10381 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 10441 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 10501 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 10561 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 10621 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 10681 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 10741 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 10801 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 10861 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 10921 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 10981 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 11041 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 11101 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 11161 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 11221 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 11281 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 11341 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 11401 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 11461 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 11521 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 11581 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 11641 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 11701 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 11761 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 11821 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 11881 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 11941 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 12001 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 12061 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 12121 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 12181 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 12241 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 12301 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 12361 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 12421 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 12481 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 12541 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc 12601 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga 12661 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 12721 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 12781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 12841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 12901 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 12961 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 13021 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 13081 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 13141 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 13201 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 13261 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 13321 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 13381 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 13441 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 13501 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 13561 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 13621 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 13681 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 13741 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 13801 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 13861 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 13921 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 13981 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 14041 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 14101 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 14161 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 14221 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 14281 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 14341 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 14401 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 14461 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 14521 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 14581 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 14641 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 14701 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 14761 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 14821 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 14881 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 14941 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 15001 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 15061 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 15121 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 15181 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 15241 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 15301 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 15361 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 15421 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 15481 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 15541 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 15601 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 15661 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 15721 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 15781 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 15841 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 15901 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 15961 tg //