LOCUS V024788 9437 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024788 VERSION V024788 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3009 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 3040..4362 /label="MAPT(NM_005910)" /note="MAPT(NM_005910)" /gene="MAPT" promoter 4407..4618 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4631..4899 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4926..5525 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5532..6120 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6194..6427 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6499..6620 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6639..6774 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6812..6828) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6836..6852 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6860..6890) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6905..6926 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7214..7802) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7973..8833) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8834..8938) /gene="bla" /label="AmpR promoter" primer_bind 9412..9428 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggtgagc aagggcgagg aggataacat ggccatcatc 2341 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 2401 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 2461 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 2521 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 2581 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 2641 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 2701 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 2761 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 2821 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 2881 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 2941 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 3001 ctgtacaagg gcggtggagg ttcaggtgga gggggatcca tggctgagcc ccgccaggag 3061 ttcgaagtga tggaagatca cgctgggacg tacgggttgg gggacaggaa agatcagggg 3121 ggctacacca tgcaccaaga ccaagagggt gacacggacg ctggcctgaa agaatctccc 3181 ctgcagaccc ccactgagga cggatctgag gaaccgggct ctgaaacctc tgatgctaag 3241 agcactccaa cagcggaaga tgtgacagca cccttagtgg atgagggagc tcccggcaag 3301 caggctgccg cgcagcccca cacggagatc ccagaaggaa ccacagctga agaagcaggc 3361 attggagaca cccccagcct ggaagacgaa gctgctggtc acgtgaccca agctcgcatg 3421 gtcagtaaaa gcaaagacgg gactggaagc gatgacaaaa aagccaaggg ggctgatggt 3481 aaaacgaaga tcgccacacc gcggggagca gcccctccag gccagaaggg ccaggccaac 3541 gccaccagga ttccagcaaa aaccccgccc gctccaaaga caccacccag ctctggtgaa 3601 cctccaaaat caggggatcg cagcggctac agcagccccg gctccccagg cactcccggc 3661 agccgctccc gcaccccgtc ccttccaacc ccacccaccc gggagcccaa gaaggtggca 3721 gtggtccgta ctccacccaa gtcgccgtct tccgccaaga gccgcctgca gacagccccc 3781 gtgcccatgc cagacctgaa gaatgtcaag tccaagatcg gctccactga gaacctgaag 3841 caccagccgg gaggcgggaa ggtgcagata attaataaga agctggatct tagcaacgtc 3901 cagtccaagt gtggctcaaa ggataatatc aaacacgtcc cgggaggcgg cagtgtgcaa 3961 atagtctaca aaccagttga cctgagcaag gtgacctcca agtgtggctc attaggcaac 4021 atccatcata aaccaggagg tggccaggtg gaagtaaaat ctgagaagct tgacttcaag 4081 gacagagtcc agtcgaagat tgggtccctg gacaatatca cccacgtccc tggcggagga 4141 aataaaaaga ttgaaaccca caagctgacc ttccgcgaga acgccaaagc caagacagac 4201 cacggggcgg agatcgtgta caagtcgcca gtggtgtctg gggacacgtc tccacggcat 4261 ctcagcaatg tctcctccac cggcagcatc gacatggtag actcgcccca gctcgccacg 4321 ctagctgacg aggtgtctgc ctccctggcc aagcagggtt tgtgagcggc cgcgaaggat 4381 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 4441 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 4501 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 4561 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 4621 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 4681 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 4741 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 4801 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 4861 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 4921 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 4981 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 5041 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 5101 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 5161 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 5221 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 5281 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 5341 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 5401 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 5461 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 5521 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 5581 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 5641 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 5701 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 5761 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 5821 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 5881 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 5941 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 6001 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 6061 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 6121 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 6181 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 6241 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6301 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6361 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6421 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 6481 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 6541 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 6601 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 6661 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 6721 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 6781 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 7021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 7081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 7141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 7201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 7261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 7681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 8041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 8101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 8161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 8221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 8281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 8341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 8701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 8761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8941 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 9001 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 9061 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 9121 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 9181 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 9241 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 9301 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 9361 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 9421 cggccagtgc caagctg //