LOCUS       V039050                11591 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039050
VERSION     V039050
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6423
                     /label="MAP3K5(NM_005923)"
                     /note="MAP3K5(NM_005923)"
                     /gene="MAP3K5"
     CDS             6430..6456
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6460..6486
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6490..6516
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6561..6772
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6785..7053
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7080..7679
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7686..8274
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8348..8581
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8653..8774
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8793..8928
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8966..8982)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8990..9006
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9014..9044)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9059..9080
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9368..9956)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10127..10987)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10988..11092)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11566..11582
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgagcacg gaggcggacg agggcatcac tttctctgtg
     2341 ccacccttcg ccccctcggg cttctgcacc atccccgagg gcggcatctg caggagggga
     2401 ggagcggcgg cggtgggcga gggcgaggag caccagctgc caccgccgcc gccgggcagc
     2461 ttctggaacg tggagagcgc cgctgcccct ggcatcggtt gtccggcggc cacctcctcg
     2521 agcagtgcca cccgaggccg gggcagctct gttggcgggg gcagccgacg gaccacggtg
     2581 gcatatgtga tcaacgaagc gagccaaggg caactggtgg tggccgagag cgaggccctg
     2641 cagagcttgc gggaggcgtg cgagacagtg ggcgccaccc tggaaaccct gcattttggg
     2701 aaactcgact ttggagaaac caccgtgctg gaccgctttt acaatgcaga tattgcggtg
     2761 gtggagatga gcgatgcctt ccggcagccg tccttgtttt accaccttgg ggtgagagaa
     2821 agtttcagca tggccaacaa catcatcctc tactgtgata ctaactcgga ctctctgcag
     2881 tcactgaagg aaataatttg ccagaagaat actatgtgca ctgggaacta cacctttgtt
     2941 ccttacatga taactccaca taacaaagtc tactgctgtg acagcagctt catgaagggg
     3001 ttgacagagc tcatgcaacc gaacttcgag ctgcttcttg gacccatctg cttacctctt
     3061 gtggatcgtt ttattcaact tttgaaggtg gcacaagcaa gttctagcca gtacttccgg
     3121 gaatctatac tcaatgacat caggaaagct cgtaatttat acactggtaa agaattggca
     3181 gctgagttgg caagaattcg gcagcgagta gataatatcg aagtcttgac agcagatatt
     3241 gtcataaatc tgttactttc ctacagagat atccaggact atgattctat tgtgaagctg
     3301 gtagagactt tagaaaaact gccaaccttt gatttggcct cccatcacca tgtgaagttt
     3361 cattatgcat ttgcactgaa taggagaaat ctccctggtg acagagcaaa agctcttgat
     3421 attatgattc ccatggtgca aagcgaagga caagttgctt cagatatgta ttgcctagtt
     3481 ggtcgaatct acaaagatat gtttttggac tctaatttca cggacactga aagcagagac
     3541 catggagctt cttggttcaa aaaggcattt gaatctgagc caacactaca gtcaggaatt
     3601 aattatgcgg tcctcctcct ggcagctgga caccagtttg aatcttcctt tgagctccgg
     3661 aaagttgggg tgaagctaag tagtcttctt ggtaaaaagg gaaacttgga aaaactccag
     3721 agctactggg aagttggatt ttttctgggg gccagcgtcc tagccaatga ccacatgaga
     3781 gtcattcaag catctgaaaa gctttttaaa ctgaagacac cagcatggta cctcaagtct
     3841 attgtagaga caattttaat atataagcat tttgtgaaac tgaccacaga acagcctgtg
     3901 gccaagcaag aacttgtgga cttttggatg gatttcctgg tcgaggccac aaagacagat
     3961 gttactgtgg ttaggtttcc agtattaata ttagaaccaa ccaaaatcta tcaaccttct
     4021 tatttgtcta tcaacaatga agttgaggaa aagacaatct ctatttggca cgtgcttcct
     4081 gatgacaaga aaggtataca tgagtggaat tttagtgcct cttctgtcag gggagtgagt
     4141 atttctaaat ttgaagaaag atgctgcttt ctttatgtgc ttcacaattc tgatgatttc
     4201 caaatctatt tctgtacaga acttcattgt aaaaagtttt ttgagatggt gaacaccatt
     4261 accgaagaga aggggagaag cacagaggaa ggagactgtg aaagtgactt gctggagtat
     4321 gactatgaat atgatgaaaa tggtgacaga gtcgttttag gaaaaggcac ttatgggata
     4381 gtctacgcag gtcgggactt gagcaaccaa gtcagaattg ctattaagga aatcccagag
     4441 agagacagca gatactctca gcccctgcat gaagaaatag cattgcataa acacctgaag
     4501 cacaaaaata ttgtccagta tctgggctct ttcagtgaga atggtttcat taaaatcttc
     4561 atggagcagg tccctggagg aagtctttct gctctccttc gttccaaatg gggtccatta
     4621 aaagacaatg agcaaacaat tggcttttat acaaagcaaa tactggaagg attaaaatat
     4681 ctccatgaca atcagatagt tcaccgggac ataaagggtg acaatgtgtt gattaatacc
     4741 tacagtggtg ttctcaagat ctctgacttc ggaacatcaa agaggcttgc tggcataaac
     4801 ccctgtactg aaacttttac tggtaccctc cagtatatgg caccagaaat aatagataaa
     4861 ggaccaagag gctacggaaa agcagcagac atctggtctc tgggctgtac aatcattgaa
     4921 atggccacag gaaaaccccc attttatgaa ctgggagaac cacaagcagc tatgttcaag
     4981 gtgggaatgt ttaaagtcca ccctgagatc ccagagtcca tgtctgcaga ggccaaggca
     5041 ttcatactga aatgttttga accagatcct gacaagagag cctgtgctaa cgacttgctt
     5101 gttgatgagt ttttaaaagt ttcaagcaaa aagaaaaaga cacaacctaa gctttcagct
     5161 ctttcagctg gatcaaatga atatctcagg agtatatcct tgccggtacc tgtgctggtg
     5221 gaggacacca gcagcagcag tgagtacggc tcagtttcac ccgacacgga gttgaaagtg
     5281 gaccccttct ctttcaaaac aagagccaag tcctgcggag aaagagatgt caagggaatt
     5341 cggacactct ttttgggcat tccagatgag aattttgaag atcacagtgc tcctccttcc
     5401 cctgaagaaa aagattctgg attcttcatg ctgaggaagg acagtgagag gcgagctacc
     5461 cttcacagga tcctgacgga agaccaagac aaaattgtga gaaacctaat ggaatcttta
     5521 gctcaggggg ctgaagaacc gaaactaaaa tgggaacaca tcacaaccct cattgcaagc
     5581 ctcagagaat ttgtgagatc cactgaccga aaaatcatag ccaccacact gtcaaagctg
     5641 aaactggagc tggacttcga cagccatggc attagccaag tccaggtggt actctttggt
     5701 tttcaagatg ctgtcaataa agttcttcgg aatcataaca tcaagccgca ctggatgttt
     5761 gccttagaca gtatcattcg gaaggcggta cagacagcca ttaccatcct ggttccagaa
     5821 ctaaggccac atttcagcct tgcatctgag agtgatactg ctgatcaaga agacttggat
     5881 gtagaagatg accatgagga acagccttca aatcaaactg tccgaagacc tcaggctgtc
     5941 attgaagatg ctgtggctac ctcaggcgtg agcacgctca gttctactgt gtctcatgat
     6001 tcccagagtg ctcaccggtc actgaatgta cagcttggaa ggatgaaaat agaaaccaat
     6061 agattactgg aagaattggt tcggaaagag aaagaattac aagcactcct tcatcgagct
     6121 attgaagaaa aagaccaaga aattaaacac ctgaagctta agtcccaacc catagaaatt
     6181 cctgaattgc ctgtatttca tctaaattct tctggcacaa atactgaaga ttctgaactt
     6241 accgactggc tgagagtgaa tggagctgat gaagacacta taagccggtt tttggctgaa
     6301 gattatacac tattggatgt tctctactat gttacacgtg atgacttaaa atgcttgaga
     6361 ctaaggggag ggatgctgtg cacactgtgg aaggctatca ttgactttcg aaacaaacag
     6421 actggatcct acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     6481 tatgctagct atccttatga tgtcccagac tacgcatagg cggccgcgaa ggatctgcga
     6541 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     6601 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     6661 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     6721 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     6781 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     6841 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     6901 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     6961 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     7021 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     7081 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     7141 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     7201 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     7261 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7321 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7381 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7441 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7501 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7561 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7621 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     7681 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7741 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7801 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7861 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7921 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7981 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8041 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8101 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     8161 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8221 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     8281 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8341 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     8401 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8461 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8521 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8581 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8641 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8701 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8761 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     8821 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8881 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     8941 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9001 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9061 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9121 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9181 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9241 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9781 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11101 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    11161 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    11221 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11281 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    11341 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    11401 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    11461 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    11521 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    11581 gtgccaagct g
//
