LOCUS       V045156                10363 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045156
VERSION     V045156
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4423
                     /label="Ltf(NM_008522)"
                     /note="Ltf(NM_008522)"
                     /gene="Ltf"
     CDS             4430..4456
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4460..4486
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4490..4516
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4561..4772
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4785..5053
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5088..5795
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5805..5858
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5859..6458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6459..7046
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7120..7353
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7425..7546
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7565..7700
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7738..7754)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7762..7778
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7786..7816)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7831..7852
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8140..8728)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8899..9759)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9760..9864)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10338..10354
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaggct gctcatccct tccttgatat ttcttgaggc
     2341 ccttggactc tgtctagcta aggcaacaac tgttcaatgg tgtgctgtgt caaattctga
     2401 ggaagaaaaa tgtttaaggt ggcagaacga gatgagaaaa gtgggtggcc cgccgctcag
     2461 ttgtgtcaag aaatcctcca cccgccagtg catccaggcc attgtgacaa acagagctga
     2521 tgccatgact cttgatggtg gcactatgtt cgatgcagga aagcccccct acaaactgcg
     2581 acctgtggca gctgaagtct acgggaccaa agagcagccc cggactcact actatgcggt
     2641 agcagtcgtg aagaacagca gtaactttca tctgaaccaa ctccaaggcc tgaggtcctg
     2701 ccacaccggc attggcagga gtgcggggtg gaaaatccct atagggacac ttcgtccata
     2761 cctgaattgg aatgggccac ctgcatccct tgaggaagcg gtatccaagt tcttctcaaa
     2821 gagctgtgtt cccggtgccc aaaaggatag attccccaac ctgtgtagct cgtgtgcagg
     2881 gacaggagcc aacaaatgtg cctcttcccc cgaggagcca tactcaggtt atgctggagc
     2941 cttgaggtgt ctgagagaca atgctggaga tgtggctttt accagaggaa gcacggtatt
     3001 tgaggagtta ccaaataaag ccgaaaggga ccagtacaag ctgctctgcc cagacaacac
     3061 ctggaagccg gtgacagaat acaaggagtg ccacctggcc caagtccctt cacatgctgt
     3121 cgtatcccga agcacgaatg acaaagaaga ggccatctgg gagcttctcc gccagtcaca
     3181 ggagaagttt ggaaaaaaac aagcatcggg attccagctc tttgcctccc cctcgggaca
     3241 gaaggacctg ctgttcaagg agtctgccat tggctttgtg agggttcccc agaaggtaga
     3301 tgtagggctc tacctgacct tcagctacac cacatccata cagaacctga ataaaaagca
     3361 gcaggatgtg atagcctcaa aggcccgggt cacatggtgt gccgtgggca gtgaggagaa
     3421 gcgcaagtgt gatcagtgga acagagcaag cagaggcagg gtcacctgca tctcattccc
     3481 caccacggaa gactgcattg tcgcaatcat gaagggagat gctgatgcca tgagcctgga
     3541 tggaggctat atctacactg cgggcaagtg cggtttagtt ccagtcttgg cagagaacca
     3601 gaaatcctcc aaaagcaatg gcttggattg tgtgaacaga ccagtggaag ggtaccttgc
     3661 tgtagcagca gttagaagag aagatgctgg cttcacctgg agctctttga gaggcaagaa
     3721 gtcctgccac actgccgtgg acaggaccgc aggctggaac atccccatgg gcctgcttgc
     3781 taaccagacc agatcctgca aatttaatga gttctttagc caaagctgtg cccctggtgc
     3841 tgaccccaaa tccaatctct gtgccctgtg tattggtgat gagaagggtg agaacaagtg
     3901 tgctcccaac agcaaagaga gataccaagg ctacactggg gctttaaggt gtctggctga
     3961 gaaggcagga aatgttgcat ttttgaagga ctccactgtc ttgcagaata ctgacgggaa
     4021 gaacactgaa gagtgggcta ggaacttaaa gctgaaggac tttgagcttt tgtgccttga
     4081 tgacacccgg aaacctgtga ctgaggctaa gaactgccac ctagccatag ccccaaacca
     4141 tgctgtagtg tctcggacag acaaggtgga agtccttcag caggtgctgc ttgaccaaca
     4201 ggttcagttt gggagaaatg gacagaggtg tccaggagag ttttgcctgt tccagtctaa
     4261 aaccaaaaac cttctgttca atgacaacac tgagtgtctg gccaagatcc ccggcaaaac
     4321 cacatcggag aagtatctgg gaaaggagta cgtcatagcg accgagcgcc tgaagcagtg
     4381 ctccagctcc ccactcctgg aagcctgcgc ttttcttacc cagggatcct acccatacga
     4441 cgtacctgat tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga
     4501 tgtcccagac tacgcatagg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt
     4561 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4621 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4681 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     4741 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     4801 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     4861 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     4921 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     4981 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     5041 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gtgagcaagg
     5101 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     5161 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5221 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     5281 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     5341 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5401 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     5461 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     5521 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     5581 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     5641 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     5701 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     5761 gccactccac cggcggcatg gacgagctgt acaagggatc tcgcgagggc agaggaagtc
     5821 ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg
     5881 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5941 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6001 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6061 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6121 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6181 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6241 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6301 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6361 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6421 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa
     6481 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6541 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6601 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6661 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6721 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6781 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6841 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6901 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6961 gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc
     7021 ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     7081 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7141 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7261 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7321 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7381 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     7561 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7621 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7681 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc
     7741 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7801 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7861 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7921 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7981 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8041 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8101 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8161 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8221 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8281 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8341 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8401 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8461 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8521 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8581 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8641 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8701 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8761 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9001 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9781 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9841 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9901 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9961 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10021 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10081 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10141 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10201 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10261 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10321 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
