LOCUS       V039606                12466 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039606
VERSION     V039606
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6492
                     /label="Lrpprc(NM_028233)"
                     /note="Lrpprc(NM_028233)"
                     /gene="Lrpprc"
     CDS             6499..6564
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6608..6819
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6832..7100
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7129..7138
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7159..7821
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7891..7944
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7945..8544
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8545..9133
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9207..9440
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9512..9633
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9673..9808
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9841..9857)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9865..9881
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9889..9919)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9934..9955
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10243..10831)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11002..11862)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11863..11967)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12441..12457
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagccctgct gagacccgcg
     2341 cgttggctgc tcggggccgc ggcggccccg cgcctcccgc tgtccctgcg cctccctgcg
     2401 ggcgtcccgg gccggctgtc ctccgtcgtc cgggtcgcgg ctgttggtag ccggccggct
     2461 gcaggagagc gtctgagcca agccagattg tatgccatcg ttgctgagaa aagggatctt
     2521 caagaggagc ctgctcctgt gagaaagaac agcagtcaat ttgactgggc tctgatgaga
     2581 ctggataatt ctgtccggag aacaggccgc atcacaaagg ggcttctgca gagagtcttt
     2641 gagagcacgt gtagctcagg tagcccaggg agcaatcaag ctctgcttct gctgcgcagc
     2701 tgtggctcgc tcctgcccga actgagtctc gccgagagga cagagtttgc tcacaagatc
     2761 tgggacaaac ttcagcagtt aggtgtcgta tatgatgtca gtcattacaa tgctttactt
     2821 aaagtatatc ttcaaaatga atacaaattt tcacctactg acttcctggc aaagatggag
     2881 ggagcaaaca tccaaccaaa tcgagtaaca taccagaggc tgatagctgc ctactgtaat
     2941 gttggggaca ttgaaggtgc cagcaagatc cttggattta tgaaaacgaa agaccttccg
     3001 atcacagagg ccgtgttcag tgctctcgtc acagggcatg cgagagctgg ggatatggaa
     3061 aatgcagaaa atattctcac agtgatgaaa caggccggca ttgagcctgg cccagacacg
     3121 tatctggcct tgttgaatgc acatgctgag aggggtgaca ttggccaggt taggcagatt
     3181 ctggagaaag tggagaagtc agaccattac ttcatggacc gcgacttctt gcaggttatt
     3241 tttagcttca gtaaggctgg ctaccctcag tatgtctcag aaatactgga gaagattacc
     3301 tatgagagac ggtctattcc agatgcaatg aacctcattt tgtttttagc cactgagaag
     3361 ttagaagaca ctgcgttcca ggttttattg gcattacccc tgtccaagga cgagagctcc
     3421 gataactttg gcagtttctt tttgcggcac tgtgtgactc tggatttgcc ccctgagaag
     3481 ctgatagact actgtcggag gctgagggac gccaagctgc acagctcctc actgcagttc
     3541 acgctgcact gtgctcttca agccaatagg acagctttgg caaaagcagt gatggaggct
     3601 ttgagggaag aagggtttcc tatccgaccg cactatttct ggccgttgct tgctgggcat
     3661 cagaaaacaa aaaatgttca aggaataata gatatcctca aaataatgaa caaagtggga
     3721 gtggatcctg atcaggaaac atatataaac tatgtgtttc cgtgctttga tagtgcacag
     3781 tcagttcgag ctgctttgca ggaaaatgaa tgtctcctcg caagtagtac ctttgctcaa
     3841 gctgaagtga agaatgaagc aataaatggg aacttacaga acattttgtc atttttggaa
     3901 tcgaatacat tgcctttctc gtttagttct ttgagaaaca gcctaatcct aggcttcagg
     3961 aggtcgatga acatagatct ttggagcaag ataacagaat tgttgtacaa ggatgaacgc
     4021 tattgctcaa agcctccggg accagcggaa gctgttggct attttcttta taacttgatt
     4081 gacagcatga gtgactcaga ggtacaggcc aaggaggagc gtttgagaca atacttccat
     4141 cagctgcagg agatgaatgt taaagttcct gaaaacatct acaaaggcat ttgtaatttg
     4201 ctgaatacct accatgttcc tgaattgatt aaggatatta aggttctggt tgacagagag
     4261 aaggtagatt ctcaaaaaac ttctcaagtt acctcatctg atttggaatc aacacttgag
     4321 aaactcaaag ctgaaggcca acctgtagga tctgccctga agcagctcct gctgctgctc
     4381 tgctcagagg agaatatgca aaaggccctt gaggtgaaag caaaatatga gtcagacatg
     4441 gttattggtg gctatgcagc attaataaat ttgtgctgtc gacatgataa tgcagaagat
     4501 gcgtggaact tgaaacaaga agttgaccgc ttagatgctt cggctattct tgacactgcc
     4561 aagtacgtag cccttgtaaa agtactggga aagcacagca gactccaaga tgctattaac
     4621 attctaaagg agatgaaaga gaaggatgtt gttatcaaag atgcaacagt cttgtccttt
     4681 ttccacatcc tcaatggtgc agctttaaga ggtgaaattg aaacagtaaa acagctgcat
     4741 gaagccatcg tgactcttgg gttggcaaag ccgtccagca acataagctt cccgttggtc
     4801 actgtgcacc tggaaaaggg tgacttacct gctgctcttg aagccagcat tgcctgccat
     4861 aaaaaatata aagtgttacc caggattcat gatgtcttat gtaagctagt agagaaaggc
     4921 gagactgatt tgatccagaa agcaatggac tttgtgagcc aagaacaagg ggagatgacg
     4981 atgctctacg acctcttctt tgctttcctg cagacgggga attacaaaga agctaagaag
     5041 atcattgaga ctccaggcat tagagctcgg cctacaagac tccagtggtt ttgtgatcga
     5101 tgcattgcca gtaatcaggt tgaagctctt gagaagttgg tagagctgac tgagaagctg
     5161 tttgagtgtg acagagacca gatgtactac aacttactga agctatacaa aataagcagt
     5221 gactggcaaa gagcggatgc tgcgtggacc aaaatgcaag aagagaacat tatccctcga
     5281 gagcggacac tgcggctctt agccgagatc ttgaaaacca gcaaccagga agttcctttc
     5341 gacgttccgg agttgtggtt tggagatgac agaccttccc tgagtccatc ctcacgctca
     5401 gcaggagagg acgttactga gaagacgttg ttgtctaact gcaaactaaa gaagagtaaa
     5461 gatgcatata atatcttcct taaagccgaa aagcaaaacg ttgtatttag cagtgaaact
     5521 tatagcacct tgataggctt gctgctgagt aaggacgact tcacccaagc aatgcacgtg
     5581 aaggatttcg ctgagaccca catcaagggc ttcacactga acgatgctgc caacagcctc
     5641 ctcatcataa ggcaagttag gcgggattat ttgaaagggg ctctggcaac tctgagagca
     5701 gccttggatt tgaagcaggt tccgtcccag atcgccgtga cccgcctcat ccaggcgttg
     5761 gccttgaagg gtgatgtgga aagcatagag gccattcaga gaatggtggc tggacttgac
     5821 acgattggac tctcaaaaat ggtttttatc aataacatcg ctttggccca gatgaagaat
     5881 aataaacttg atgctgccat agaaaacatt gagcacctgc ttgcttccga gaaccaagcc
     5941 atagaacctc agtactttgg cttgtcgtat ctattcagaa aagtgatcga agagcagatg
     6001 gaaccagcgc tagagaagtt aagcatcatg tctgagagaa tggcgaatca gtttgcactt
     6061 tacaagcccg tcactgatct attcctgcag cttgtggatt caggcaaggt ggatgaggcc
     6121 agagctctct tagagagatg cggtgccatt gccgagcaga gctcgcttct gtcggtgttc
     6181 tgtctgagga cttctcagaa accgaaaaag gcaccagttc tgaagacttt gttagaactg
     6241 attcctgagt tacgtgataa cgataaagta tattcttgca gcatgaaaag ctatgcctta
     6301 gacaaagatg tggcctcggc taaagcactg tatgagtatt tgacagccaa gaacttgaag
     6361 ctagatgacc tgtttctcaa gcgctatgca gctttgctca aggatgtcgg cgaaccagtc
     6421 cccttccccg agccccctga aagctttgca ttttatataa agcaactaaa ggaagcaagg
     6481 gaaagccctt caggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     6541 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     6601 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6661 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6721 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6781 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     6841 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6901 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6961 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     7021 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     7081 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     7141 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     7201 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     7261 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     7321 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     7381 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     7441 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     7501 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     7561 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     7621 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     7681 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7741 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7801 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     7861 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7921 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7981 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     8041 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     8101 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     8161 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     8221 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     8281 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     8341 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8401 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8461 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8521 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     8581 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8641 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8701 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8761 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8821 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8881 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8941 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9001 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9061 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9121 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     9241 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9421 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9481 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9541 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9601 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     9661 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9721 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9781 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     9841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10681 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    12001 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    12061 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    12121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    12181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    12241 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    12301 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    12361 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    12421 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
