LOCUS V033272 10567 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033272 VERSION V033272 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4593 /label="LONRF3(NM_001031855)" /note="LONRF3(NM_001031855)" /gene="LONRF3" CDS 4600..4665 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4709..4920 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4933..5201 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5230..5239 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5260..5922 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5992..6045 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6046..6645 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6646..7234 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7308..7541 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7613..7734 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7774..7909 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7942..7958) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7966..7982 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7990..8020) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8035..8056 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8344..8932) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9103..9963) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9964..10068) /gene="bla" /label="AmpR promoter" primer_bind 10542..10558 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agtcagtacg gatcgaacag 2341 atgctgagct tgcccgctga ggtcagcagc gacaacttgg agtcggcgga gcgaggggca 2401 tcagcggccc aagtagacat gggcccccac ccaaaggtgg ctgcagaggg ccccgcacct 2461 ctaccgacgc gggagccaga gcaagagcag tctccgggga cctcaacgcc ggagagcaaa 2521 gtcctgctca cgcaggcaga cgccttggcg tcccgggggc gaatccgtga agccctcgag 2581 gtgtatagac agctctccga gcggcagcag ctggtggctg agcagctgga gcagctggtg 2641 cgctgcctgg cggagaaagt cccgcaaggc gaggcgctgg cgccggcgcc cccggacgag 2701 ggtagcactg caagcggcac cgtggcggcg gaagagacgg gggccgccgc ggctgcggcg 2761 gccaccgagg tgtgggacgg ctttaagtgc cggaaatgtc atgggtttct atcagacccc 2821 gtgtccttgt cgtgtggcca caccttttgt aaactgtgcc tggaacgtgg gcgggccgcc 2881 gaccggcgct gtgcgctgtg cggggtcaag ctctccgcct tgatggtggc cactgggcgg 2941 gcgcgtggag cccggcgggc tgggcagcag ccgccgccgc cgctgcgagt caacgtggtg 3001 ctcagcggcc tcctcggcaa gttgtttcca ggcccagcgc gagcgtcgca actccggcac 3061 gagggcaacc gactgtaccg cgagcgccag gtggaggcgg cactgctcaa gtacaacgag 3121 gcagttaagt tggctccaaa tgaccacttg ctttatagca atcggtctca gatttatttc 3181 accttggagt ctcatgagaa tgcactgcat gatgcagaaa tagcatgtaa gctccgcccg 3241 atgggtttta aggcacattt cagaaaagcc caagccttag ccaccctagg caaggtggag 3301 gaggcactaa gggagtttct ctactgtgta tcccttgatg gaaagaacaa gagagcaaga 3361 tgtgaagccc aaagagacaa tctggagctc ccacattgtt ctagtcagga ggaagcagca 3421 gccaggggag atggcagcag tctgatggac ccagctaaag tgaaggggga tggtcagcag 3481 caccacatga aagaccagga agaagaggag gagaagtggg atgctacctc tccaaaagct 3541 gcttccagca agactggaaa atgccaggaa aagaaaagga aacattgcca gattgaatcc 3601 caagaagaaa cggggatgcc taataaagcc tccaagcaag atcctcccac tgatcagggg 3661 gacaaacctg ctctcagttt accacttgca tctttcgacg catctgacct tgaatgcgct 3721 ctatgtatga gattattcta tgagccagtc acaacacctt gcgggcatac tttttgctta 3781 aaatgcctag aaagatgcct agatcacaac gcaaagtgtc cattgtgcaa agacggtctt 3841 tcacagtgct tggcatcaag aaaatacagc aaaaatgtaa taatggagga gctcatagct 3901 aaattccttc cagaagaact gaaggaacga aggaagcttt atgaagagga aatggaagaa 3961 ctttctaacc ttaataagaa tgtgcctatt ttcgtgtgta ctatggccta tcccaccgtt 4021 ccttgtcccc tgcacatctt tgagccttgt taccgcctga tgattcgtag atgcattgag 4081 acaggcacga gacagtttgg catgtgcctt ggagatcctg tcaaagggtt tgcagaatat 4141 ggctgcatcc tagagatcag aaatgttcaa ttctttgctg atggccgctc agtggttgac 4201 agcataggca agaggcgctt cagggtgctc catcagagcc agcgggatgg ctacaacaca 4261 gccgacattg aatacattga agaccaaaag gttcagggag aggattgtgc tgagctcatg 4321 ggattacata actgtgtcta tcagcaagca tcattgtggt ttcattcgct caaattatcc 4381 ctaaagaatc ggatactcaa tcactttggt cccatgccgg agaaagacgc cgatcctcag 4441 atgaacccga atggcccagc ctggtgctgg tggatgttag cagttcttcc cttggaaagc 4501 cgagctcagc tccccttcct agcaatgagg tccttaaagg acagactgaa tggtattcga 4561 cgagtcctgg ccttcatatc ccgaaaccaa aacggatccg actacaaaga ccatgacggt 4621 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg 4681 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 4741 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 4801 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 4861 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4921 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4981 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 5041 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 5101 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 5161 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 5221 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 5281 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 5341 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 5401 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 5461 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 5521 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 5581 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 5641 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 5701 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 5761 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 5821 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 5881 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 5941 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 6001 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 6061 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 6121 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 6181 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 6241 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 6301 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 6361 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 6421 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 6481 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 6541 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 6601 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 6661 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6721 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6781 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 6841 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 6901 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6961 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 7021 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 7081 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 7141 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 7201 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta 7261 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 7321 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca 7381 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 7441 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 7501 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7561 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 7621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 7681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 7741 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7801 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7861 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 7921 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 7981 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 8041 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 8101 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 8161 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8221 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8281 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8341 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8401 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8461 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8521 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8581 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8641 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8761 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8821 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 9001 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 9061 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 9121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 10021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 10081 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 10141 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 10201 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 10261 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 10321 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 10381 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 10441 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 10501 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10561 caagctg //