LOCUS       V029605                10324 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029605
VERSION     V029605
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5178
                     /label="LONP1(NM_004793)"
                     /note="LONP1(NM_004793)"
                     /gene="LONP1"
     CDS             5185..5250
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5294..5505
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5518..5786
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5813..6412
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6419..7007
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7081..7314
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7386..7507
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7526..7661
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7699..7715)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7723..7739
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7747..7777)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7792..7813
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8860..9720)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9721..9825)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10299..10315
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggcg agcactggct acgtgcgact gtggggagcg
     2341 gcgcggtgct gggtgctgcg gcggccgatg ctggccgccg ccggggggcg ggttcccact
     2401 gcagcaggag cgtggttgct ccgaggccag cggacctgcg acgcctctcc tccttgggca
     2461 ctgtggggcc gaggcccggc aattgggggc caatggcggg ggttttggga agcgagcagc
     2521 cgcggcggag gcgcattctc ggggggcgag gacgcctccg agggcggcgc ggaggaagga
     2581 gccggcggcg cggggggcag cgcgggcgcc ggggaaggcc cggtcataac ggcgctcacg
     2641 cccatgacga tccccgatgt gtttccgcac ctgccgctca tcgccatcac ccgcaacccg
     2701 gtgttcccgc gctttatcaa gattatcgag gttaaaaata agaagttggt tgagctgctg
     2761 agaaggaaag ttcgtctcgc ccagccttat gtcggcgtct ttctaaagag agatgacagc
     2821 aatgagtcgg atgtggtcga gagcctggat gaaatctacc acacggggac gtttgcccag
     2881 atccatgaga tgcaggacct tggggacaag ctgcgcatga tcgtcatggg acacagaaga
     2941 gtccatatca gcagacagct ggaggtggag cccgaggagc cggaggcgga gaacaagcac
     3001 aagccccgca ggaagtcaaa gcggggcaag aaggaggcgg aggacgagct gagcgccagg
     3061 cacccggcgg agctggcgat ggagcccacc cctgagctcc cggctgaggt gctcatggtg
     3121 gaggtagaga acgttgtcca cgaggacttc caggtcacgg aggaggtgaa agccctgact
     3181 gcagagatcg tgaagaccat ccgggacatc attgccttga accctctcta cagggagtca
     3241 gtgctgcaga tgatgcaggc tggccagcgg gtggtggaca accccatcta cctgagcgac
     3301 atgggcgccg cgctcaccgg ggccgagtcc catgagctgc aggacgtcct ggaagagacc
     3361 aatattccta agcggctgta caaggccctc tccctgctga agaaggaatt tgaactgagc
     3421 aagctgcagc agcgcctggg gcgggaggtg gaggagaaga tcaagcagac ccaccgtaag
     3481 tacctgctgc aggagcagct aaagatcatc aagaaggagc tgggcctgga gaaggacgac
     3541 aaggatgcca tcgaggagaa gttccgggag cgcctgaagg agctcgtggt ccccaagcac
     3601 gtcatggatg ttgtggacga ggagctgagc aagctgggcc tgctggacaa ccactcctcg
     3661 gagttcaatg tcacccgcaa ctacctagac tggctcacgt ccatcccttg gggcaagtac
     3721 agcaacgaga acctggacct ggcgcgggca caggcagtgc tggaggaaga ccactacggc
     3781 atggaggacg tcaagaaacg catcctggag ttcattgccg ttagccagct ccgcggctcc
     3841 acccagggca agatcctctg cttctatggc ccccctggcg tgggtaagac cagcattgct
     3901 cgctccatcg cccgcgccct gaaccgagag tacttccgct tcagcgtcgg gggcatgact
     3961 gacgtggctg agatcaaggg ccacaggcgg acctacgtgg gcgccatgcc cgggaagatc
     4021 atccagtgtt tgaagaagac caagacggag aaccccctga tcctcatcga cgaggtggac
     4081 aagatcggcc gaggctacca gggggacccg tcgtcggcac tgctggagct gctggaccca
     4141 gagcagaatg ccaacttcct ggaccactac ctggacgtgc ccgtggactt gtccaaggtg
     4201 ctgttcatct gcacggccaa cgtcacggac accatccccg agccgctgcg agaccgtatg
     4261 gagatgatca acgtgtcggg ctacgtggcc caggagaagc tggccattgc ggagcgctac
     4321 ctggtgcccc aggctcgcgc cctgtgtggc ttggatgaga gcaaggccaa gctgtcatcg
     4381 gacgtgctga cgctgctcat caagcagtac tgccgcgaga gcggtgtccg caacctgcag
     4441 aagcaagtgg agaaggtgtt acggaaatcg gcctacaaga ttgtcagcgg cgaggccgag
     4501 tccgtggagg tgacgcccga gaacctgcag gacttcgtgg ggaagcccgt gttcaccgtg
     4561 gagcgcatgt atgacgtgac accgcccggc gtggtcatgg ggctggcctg gaccgcaatg
     4621 ggaggctcca cgctgtttgt ggagacatcc ctgagacggc cacaggacaa ggatgccaag
     4681 ggtgacaagg atggcagcct ggaggtgaca ggccagctgg gggaggtgat gaaggagagc
     4741 gcccgcatag cctacacctt cgccagagcc ttcctcatgc agcacgcccc cgccaatgac
     4801 tacctggtga cctcacacat ccacctgcat gtgcccgagg gcgccacccc caaggacggc
     4861 ccaagcgcag gctgcaccat cgtcacggcc ctgctgtccc tggccatggg caggcctgtc
     4921 cggcagaatc tggccatgac tggcgaagtc tccctcacgg gcaagatcct gcctgttggt
     4981 ggcatcaagg agaagaccat tgcggccaag cgcgcagggg tgacgtgcat cgtcctgcca
     5041 gccgagaaca agaaggactt ctacgacctg gcagccttca tcaccgaggg cctggaggtg
     5101 cacttcgtgg aacactaccg ggagatcttc gacatcgcct tcccggacga gcaggcagag
     5161 gcgctggccg tggaacgggg atccgactac aaagaccatg acggtgatta taaagatcat
     5221 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5281 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5341 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5401 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5461 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5521 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5581 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5641 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5701 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5761 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     5821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     5881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     5941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6001 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6361 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     6421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6781 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6961 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     7021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7081 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     7141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7321 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7381 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7501 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     7561 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7621 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     7681 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9841 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9901 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10081 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10141 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10201 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10261 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10321 gctg
//
