LOCUS       V022771                11386 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022771
VERSION     V022771
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5428
                     /label="KLB(NM_175737)"
                     /note="KLB(NM_175737)"
                     /gene="KLB"
     CDS             5435..5500
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5545..5756
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5769..6037
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6066..6075
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6096..6758
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6828..6881
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6882..7481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7482..8069
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8143..8376
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8448..8569
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8588..8723
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8761..8777)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8785..8801
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8809..8839)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8854..8875
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9163..9751)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9922..10782)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10783..10887)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11361..11377
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga agccaggctg tgcggcagga tctccaggga atgaatggat
     2341 tttcttcagc actgatgaaa taaccacacg ctataggaat acaatgtcca acgggggatt
     2401 gcaaagatct gtcatcctgt cagcacttat tctgctacga gctgttactg gattctctgg
     2461 agatggaaga gctatatggt ctaaaaatcc taattttact ccggtaaatg aaagtcagct
     2521 gtttctctat gacactttcc ctaaaaactt tttctggggt attgggactg gagcattgca
     2581 agtggaaggg agttggaaga aggatggaaa aggaccttct atatgggatc atttcatcca
     2641 cacacacctt aaaaatgtca gcagcacgaa tggttccagt gacagttata tttttctgga
     2701 aaaagactta tcagccctgg attttatagg agtttctttt tatcaatttt caatttcctg
     2761 gccaaggctt ttccccgatg gaatagtaac agttgccaac gcaaaaggtc tgcagtacta
     2821 cagtactctt ctggacgctc tagtgcttag aaacattgaa cctatagtta ctttatacca
     2881 ctgggatttg cctttggcac tacaagaaaa atatgggggg tggaaaaatg ataccataat
     2941 agatatcttc aatgactatg ccacatactg tttccagatg tttggggacc gtgtcaaata
     3001 ttggattaca attcacaacc catatctagt ggcttggcat gggtatggga caggtatgca
     3061 tgcccctgga gagaagggaa atttagcagc tgtctacact gtgggacaca acttgatcaa
     3121 ggctcactcg aaagtttggc ataactacaa cacacatttc cgcccacatc agaagggttg
     3181 gttatcgatc acgttgggat ctcattggat cgagccaaac cggtcggaaa acacgatgga
     3241 tatattcaaa tgtcaacaat ccatggtttc tgtgcttgga tggtttgcca accctatcca
     3301 tggggatggc gactatccag aggggatgag aaagaagttg ttctccgttc tacccatttt
     3361 ctctgaagca gagaagcatg agatgagagg cacagctgat ttctttgcct tttcttttgg
     3421 acccaacaac ttcaagcccc taaacaccat ggctaaaatg ggacaaaatg tttcacttaa
     3481 tttaagagaa gcgctgaact ggattaaact ggaatacaac aaccctcgaa tcttgattgc
     3541 tgagaatggc tggttcacag acagtcgtgt gaaaacagaa gacaccacgg ccatctacat
     3601 gatgaagaat ttcctcagcc aggtgcttca agcaataagg ttagatgaaa tacgagtgtt
     3661 tggttatact gcctggtctc tcctggatgg ctttgaatgg caggatgctt acaccatccg
     3721 ccgaggatta ttttatgtgg attttaacag taaacagaaa gagcggaaac ctaagtcttc
     3781 agcacactac tacaaacaga tcatacgaga aaatggtttt tctttaaaag agtccacgcc
     3841 agatgtgcag ggccagtttc cctgtgactt ctcctggggt gtcactgaat ctgttcttaa
     3901 gcccgagtct gtggcttcgt ccccacagtt cagcgatcct catctgtacg tgtggaacgc
     3961 cactggcaac agactgttgc accgagtgga aggggtgagg ctgaaaacac gacccgctca
     4021 atgcacagat tttgtaaaca tcaaaaaaca acttgagatg ttggcaagaa tgaaagtcac
     4081 ccactaccgg tttgctctgg attgggcctc ggtccttccc actggcaacc tgtccgcggt
     4141 gaaccgacag gccctgaggt actacaggtg cgtggtcagt gaggggctga agcttggcat
     4201 ctccgcgatg gtcaccctgt attatccgac ccacgcccac ctaggcctcc ccgagcctct
     4261 gttgcatgcc gacgggtggc tgaacccatc gacggccgag gccttccagg cctacgctgg
     4321 gctgtgcttc caggagctgg gggacctggt gaagctctgg atcaccatca acgagcctaa
     4381 ccggctaagt gacatctaca accgctctgg caacgacacc tacggggcgg cgcacaacct
     4441 gctggtggcc cacgccctgg cctggcgcct ctacgaccgg cagttcaggc cctcacagcg
     4501 cggggccgtg tcgctgtcgc tgcacgcgga ctgggcggaa cccgccaacc cctatgctga
     4561 ctcgcactgg agggcggccg agcgcttcct gcagttcgag atcgcctggt tcgccgagcc
     4621 gctcttcaag accggggact accccgcggc catgagggaa tacattgcct ccaagcaccg
     4681 acgggggctt tccagctcgg ccctgccgcg cctcaccgag gccgaaagga ggctgctcaa
     4741 gggcacggtc gacttctgcg cgctcaacca cttcaccact aggttcgtga tgcacgagca
     4801 gctggccggc agccgctacg actcggacag ggacatccag tttctgcagg acatcacccg
     4861 cctgagctcc cccacgcgcc tggctgtgat tccctggggg gtgcgcaagc tgctgcggtg
     4921 ggtccggagg aactacggcg acatggacat ttacatcacc gccagtggca tcgacgacca
     4981 ggctctggag gatgaccggc tccggaagta ctacctaggg aagtaccttc aggaggtgct
     5041 gaaagcatac ctgattgata aagtcagaat caaaggctat tatgcattca aactggctga
     5101 agagaaatct aaacccagat ttggattctt cacatctgat tttaaagcta aatcctcaat
     5161 acaattttac aacaaagtga tcagcagcag gggcttccct tttgagaaca gtagttctag
     5221 atgcagtcag acccaagaaa atacagagtg cactgtctgc ttattccttg tgcagaagaa
     5281 accactgata ttcctgggtt gttgcttctt ctccaccctg gttctactct tatcaattgc
     5341 catttttcaa aggcagaaga gaagaaagtt ttggaaagca aaaaacttac aacacatacc
     5401 attaaagaaa ggcaagagag ttgttagcgg atccgactac aaagaccatg acggtgatta
     5461 taaagatcat gacatcgact acaaggatga cgatgacaag tgagcggccg cgaaggatct
     5521 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     5581 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     5641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     5701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     5761 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     5821 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     5881 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     5941 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     6001 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     6061 tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca
     6121 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca
     6181 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct
     6241 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct
     6301 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga
     6361 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg
     6421 tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg
     6481 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc
     6541 tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg
     6601 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc
     6661 ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg
     6721 agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca
     6781 attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa
     6841 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca
     6901 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6961 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     7021 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     7081 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     7141 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     7201 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7261 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7321 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7381 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7441 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca
     7501 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7561 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7621 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7681 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7741 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7801 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7861 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7921 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7981 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     8041 gtcggatctc cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     8101 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8161 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8221 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8281 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8341 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     8401 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8461 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8581 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8701 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     8761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9601 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9721 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9781 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9841 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9901 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9961 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10021 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10081 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10141 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10201 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10261 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10321 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10381 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10441 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10501 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10561 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10621 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10681 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10741 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10801 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10861 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10921 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10981 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11041 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11101 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11161 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11221 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11281 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11341 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
