LOCUS       V034312                10435 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034312
VERSION     V034312
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5337
                     /label="KL(NM_004795)"
                     /note="KL(NM_004795)"
                     /gene="KL"
     CDS             5344..5361
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        5405..5616
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5629..5897
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5924..6523
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6530..7118
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7192..7425
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7497..7618
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7637..7772
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7810..7826)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7834..7850
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7858..7888)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7903..7924
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8212..8800)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8971..9831)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9832..9936)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10410..10426
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcccgcc agcgccccgc cgcgccgccc gcggccgccg
     2341 ccgccgtcgc tgtcgctgct gctggtgctg ctgggcctgg gcggccgccg cctgcgtgcg
     2401 gagccgggcg acggcgcgca gacctgggcc cgtttctcgc ggcctcctgc ccccgaggcc
     2461 gcgggcctct tccagggcac cttccccgac ggcttcctct gggccgtggg cagcgccgcc
     2521 taccagaccg agggcggctg gcagcagcac ggcaagggtg cgtccatctg ggatacgttc
     2581 acccaccacc ccctggcacc cccgggagac tcccggaacg ccagtctgcc gttgggcgcc
     2641 ccgtcgccgc tgcagcccgc caccggggac gtagccagcg acagctacaa caacgtcttc
     2701 cgcgacacgg aggcgctgcg cgagctcggg gtcactcact accgcttctc catctcgtgg
     2761 gcgcgagtgc tccccaatgg cagcgcgggc gtccccaacc gcgaggggct gcgctactac
     2821 cggcgcctgc tggagcggct gcgggagctg ggcgtgcagc ccgtggtcac cctgtaccac
     2881 tgggacctgc cccagcgcct gcaggacgcc tacggcggct gggccaaccg cgccctggcc
     2941 gaccacttca gggattacgc ggagctctgc ttccgccact tcggcggtca ggtcaagtac
     3001 tggatcacca tcgacaaccc ctacgtggtg gcctggcacg gctacgccac cgggcgcctg
     3061 gcccccggca tccggggcag cccgcggctc gggtacctgg tggcgcacaa cctcctcctg
     3121 gctcatgcca aagtctggca tctctacaat acttctttcc gtcccactca gggaggtcag
     3181 gtgtccattg ccctaagctc tcactggatc aatcctcgaa gaatgaccga ccacagcatc
     3241 aaagaatgtc aaaaatctct ggactttgta ctaggttggt ttgccaaacc cgtatttatt
     3301 gatggtgact atcccgagag catgaagaat aacctttcat ctattctgcc tgattttact
     3361 gaatctgaga aaaagttcat caaaggaact gctgactttt ttgctctttg ctttggaccc
     3421 accttgagtt ttcaactttt ggaccctcac atgaagttcc gccaattgga atctcccaac
     3481 ctgaggcaac tgctttcctg gattgacctt gaatttaacc atcctcaaat atttattgtg
     3541 gaaaatggct ggtttgtctc agggaccacc aagagagatg atgccaaata tatgtattac
     3601 ctcaaaaagt tcatcatgga aaccttaaaa gccatcaagc tggatggggt ggatgtcatc
     3661 gggtataccg catggtccct catggatggt ttcgagtggc acagaggtta cagcatcagg
     3721 cgtggactct tctatgttga ctttctaagc caggacaaga tgttgttgcc aaagtcttca
     3781 gccttgttct accaaaagct gatagagaaa aatggcttcc ctcctttacc tgaaaatcag
     3841 cccctagaag ggacatttcc ctgtgacttt gcttggggag ttgttgacaa ctacattcaa
     3901 gtagatacca ctctgtctca gtttaccgac ctgaatgttt acctgtggga tgtccaccac
     3961 agtaaaaggc ttattaaagt ggatggggtt gtgaccaaga agaggaaatc ctactgtgtt
     4021 gactttgctg ccatccagcc ccagatcgct ttactccagg aaatgcacgt tacacatttt
     4081 cgcttctccc tggactgggc cctgattctc cctctgggta accagtccca ggtgaaccac
     4141 accatcctgc agtactatcg ctgcatggcc agcgagcttg tccgtgtcaa catcacccca
     4201 gtggtggccc tgtggcagcc tatggccccg aaccaaggac tgccgcgcct cctggccagg
     4261 cagggcgcct gggagaaccc ctacactgcc ctggcctttg cagagtatgc ccgactgtgc
     4321 tttcaagagc tcggccatca cgtcaagctt tggataacga tgaatgagcc gtatacaagg
     4381 aatatgacat acagtgctgg ccacaacctt ctgaaggccc atgccctggc ttggcatgtg
     4441 tacaatgaaa agtttaggca tgctcagaat gggaaaatat ccatagcctt gcaggctgat
     4501 tggatagaac ctgcctgccc tttctcccaa aaggacaaag aggtggctga gagagttttg
     4561 gaatttgaca ttggctggct ggctgagccc attttcggct ctggagatta tccatgggtg
     4621 atgagggact ggctgaacca aagaaacaat tttcttcttc cttatttcac tgaagatgaa
     4681 aaaaagctaa tccagggtac ctttgacttt ttggctttaa gccattatac caccatcctt
     4741 gtagactcag aaaaagaaga tccaataaaa tacaatgatt acctagaagt gcaagaaatg
     4801 accgacatca cgtggctcaa ctcccccagt caggtggcgg tagtgccctg ggggttgcgc
     4861 aaagtgctga actggctgaa gttcaagtac ggagacctcc ccatgtacat aatatccaat
     4921 ggaatcgatg acgggctgca tgctgaggac gaccagctga gggtgtatta tatgcagaat
     4981 tacataaacg aagctctcaa agcccacata ctggatggta tcaatctttg cggatacttt
     5041 gcttattcgt ttaacgaccg cacagctccg aggtttggcc tctatcgtta tgctgcagat
     5101 cagtttgagc ccaaggcatc catgaaacat tacaggaaaa ttattgacag caatggtttc
     5161 ccgggcccag aaactctgga aagattttgt ccagaagaat tcaccgtgtg tactgagtgc
     5221 agtttttttc acacccgaaa gtctttactg gctttcatag cttttctatt ttttgcttct
     5281 attatttctc tctcccttat attttactac tcgaagaaag gcagaagaag ttacaaagga
     5341 tcccatcacc atcaccatca ctagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5401 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5461 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5521 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5581 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5641 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5701 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5761 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5821 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5881 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     5941 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     6001 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     6061 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6121 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6181 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6241 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6301 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6361 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6421 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6481 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     6541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7081 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     7141 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7201 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     7261 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7321 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7381 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7441 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7501 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7561 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7621 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7681 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7741 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     7801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10021 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10141 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10201 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10261 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10321 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10381 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
